Unreviewed, UniProtKB/TrEMBL A0A4M4 (A0A4M4_9INFA)

Names and origin

Protein names

Submitted name:
    Nucleoprotein EMBL ABJ90249.1

Gene names

Name:

NP EMBL ABJ90249.1

Organism

Influenza A virus (A/chicken/Italy/5093/99(H7N1)) EMBL ABJ90249.1

Taxonomic identifier

414453 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus. Spearmint SPM002141
Subunit structure	Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases. Spearmint SPM002141
Subcellular location	VirionPotential. Nucleus. Spearmint SPM002141

Ontologies

Keywords
Biological process	Host-virus interaction Spearmint SPM002141
Cellular component	Nucleus Spearmint SPM002141 Virion
Ligand	RNA-binding Spearmint SPM002141 Viral nucleoprotein
Gene Ontology (GO)
Biological process	interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW virion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A0A4M4-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 2DA71D44F7E54769	FASTA	497	56,178
	10 20 30 40 50 60 MASQGTKRSY EQMETGERQN ATEIRASVGR MVGGIGRFYI QMCTELKLSD YEGRLIQNSI 70 80 90 100 110 120 TIERMVLSAF DERRNKYLEE HPSAGKDPKK TGGPIYRRRD GKWVRELILY DKEEIRRIWR 130 140 150 160 170 180 QANNGEDATA GLTHLMIWHS NLNDATYQRT RALVRTGMDP RMCSLMQGST LPRRSGAAGA 190 200 210 220 230 240 AVKGVGTMVM ELIRMIKRGV NDRNFWRGEN GRRTRIAYER MCNILKGKFQ TAAQRAMMDQ 250 260 270 280 290 300 VRESRNPGNA EIEDLIFLAR SALILRGSVA HKSCLPACVY GLAVASGYDF EREGYSLVGI 310 320 330 340 350 360 DPFRLLQNSQ VFSLIRPNEN PAHKSQLVWM ACHSAAFEDL RVSSFIRGAR VVPRGQLSTR 370 380 390 400 410 420 GVQIASNENM ETMDSNTLEL RSRYWAIRTR SGGNTNQQRA SAGQISVQPT FSVQRNLPFE 430 440 450 460 470 480 RATIMAAFTG NTEGRTSDMR TEIIRMMESA RPEDVSFQGR GVFELSDEKA TNPIVPSFDM 490 SNEGSYFFGD NAEEYDN

References

[1]

"Pneumo- and neurotropism of avian origin Italian highly pathogenic avian influenza H7N1 isolates in experimentally infected mice." Rigoni M., Shinya K., Toffan A., Milani A., Bettini F., Kawaoka Y., Cattoli G., Capua I. Virology 364:28-35(2007) [PubMed: 17408714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: A/chicken/Italy/5093/99 EMBL ABJ90249.1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ991321 Viral cRNA. Translation: ABJ90249.1. DQ991329 Viral cRNA. Translation: ABJ90260.2.
3D structure databases
SMR	A0A4M4. Positions 20-488.
ModBase	Search...
Family and domain databases
InterPro	IPR002141. Flu_NP. [Graphical view]
Pfam	PF00506. Flu_NP. 1 hit. [Graphical view]
ProDom	A0A4M4. [Graphical view] [Entries sharing at least one domain]

Entry information

Entry name

A0A4M4_9INFA

Accession

Primary (citable) accession number: A0A4M4
Secondary accession number(s): A0A4N5

Entry history

Integrated into UniProtKB/TrEMBL:	November 14, 2006
Last sequence update:	November 14, 2006
Last modified:	September 23, 2008
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)