Reviewed, UniProtKB/Swiss-Prot P07456 (IGF2_BOVIN)

Names and origin

Protein names

Recommended name:
    Insulin-like growth factor II
      Short name(s)=IGF-II
Alternative name(s):
    Erythrotropin

Gene names

Name:

IGF2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Subcellular location	Secreted.
Sequence similarities	Belongs to the insulin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Growth factor Mitogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 24	24
	Chain	25 – 91	67	Insulin-like growth factor II
	Propeptide	92 – 179	88	E peptide
Regions
	Region	25 – 52	28	B
	Region	53 – 64	12	C
	Region	65 – 85	21	A
	Region	86 – 91	6	D
Amino acid modifications
	Disulfide bond	33 ↔ 71		By similarity
	Disulfide bond	45 ↔ 84		By similarity
	Disulfide bond	70 ↔ 75		By similarity
Experimental info
	Sequence conflict	46 – 47	2	GD → DG AA sequence Ref.5
	Sequence conflict	58	1	R → L in ABD34310. Ref.1
	Sequence conflict	59	1	I → S AA sequence Ref.3
	Sequence conflict	168	1	T → I in CAA37620. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P07456-1 [UniParc]. Last modified May 30, 2006. Version 4. Checksum: DEA16ED2D792EA53	FASTA	179	19,682
	10 20 30 40 50 60 MGITAGKSVL VLLAFLAFAS CCYAAYRPSE TLCGGELVDT LQFVCGDRGF YFSRPSSRIN 70 80 90 100 110 120 RRSRGIVEEC CFRSCDLALL ETYCATPAKS ERDVSASTTV LPDDVTAYPV GKFFQYDIWK 130 140 150 160 170 QSTQRLRRGL PAFLRARRGR TLAKELEALR EAKSHRPLIA LPTQDPATHG GASSKASSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Promoter-specific expression of the imprinted IGF2 gene in cattle (Bos taurus)." Curchoe C., Zhang S., Bin Y., Zhang X., Yang L., Feng D., O'Neill M., Tian X.C. Biol. Reprod. 73:1275-1281(2005) [PubMed: 16120826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Ascending colon.
[3]	"Insulin-like growth factors I and II in fetal and adult bovine serum. Purification, primary structures, and immunological cross-reactivities." Honegger A., Humbel R.E. J. Biol. Chem. 261:569-575(1986) [PubMed: 3941093] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-91.
[4]	"Insulin-like growth factors 1 and 2 in bovine colostrum. Sequences and biological activities compared with those of a potent truncated form." Francis G.L., Upton F.M., Ballard F.J., McNeil K.A., Wallace J.C. Biochem. J. 251:95-103(1988) [PubMed: 3390164] [Abstract] Cited for: SEQUENCE REVISION.
[5]	"A heparin-binding erythroid cell stimulating factor from fetal bovine serum has the N-terminal sequence of insulin-like growth factor II." Li Q., Blacher R., Esch F., Congote L.F. Biochem. Biophys. Res. Commun. 166:557-561(1990) [PubMed: 2302223] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-55.
[6]	"The nucleotide and deduced amino acid sequences of insulin-like growth factor II cDNAs from adult bovine and fetal sheep liver." Brown W.M., Dziegielewska K.M., Foreman R.C., Saunders N.R. Nucleic Acids Res. 18:4614-4614(1990) [PubMed: 2388846] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-179. Tissue: Liver.
[7]	"Nucleotide sequence of the central coding region of bovine erythrotropin/insulin-like growth factor II cDNA from fetal intestine and northern analysis of the major IGF II transcripts at the time of hepatic erythropoiesis." Congote L.F., Mazza L., Palfree R.G.E. Comp. Biochem. Physiol. 103B:127-131(1992) [PubMed: 1280544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-86.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY957981 mRNA. Translation: ABD34310.1. BC116039 mRNA. Translation: AAI16040.1. X53553 mRNA. Translation: CAA37620.1. X53867 mRNA. Translation: CAA37861.1.
PIR	IGBO2. S10983.
RefSeq	NP_776512.2.
UniGene	Bt.98687
3D structure databases
HSSP	HSSP built from PDB template 1IGL based on UniProtKB P01344.
SMR	P07456. Positions 25-91.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000013066. Bos taurus. [Contig view]
GeneID	281240.
KEGG	bta:281240.
Phylogenomic databases
HOVERGEN	P07456.
Family and domain databases
InterPro	IPR013576. IGF2_C. IPR004825. Ins/IGF/relaxin. [Graphical view]
Gene3D	G3DSA:1.10.100.10. Ins/IGF/relaxin. 1 hit.
Pfam	PF08365. IGF2_C. 1 hit. PF00049. Insulin. 1 hit. [Graphical view]
PRINTS	PR00277. INSULINB.
SMART	SM00078. IlGF. 1 hit. [Graphical view]
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
ProDom	P07456. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

IGF2_BOVIN

Accession

Primary (citable) accession number: P07456
Secondary accession number(s): A0A5F2, Q2HJN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	May 30, 2006
Last modified:	July 22, 2008
This is version 72 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)