Reviewed, UniProtKB/Swiss-Prot A2AL36 (CP110_MOUSE)

Names and origin

Protein names

Recommended name:
    Centriolin
Alternative name(s):
    110 kDa centrosomal protein
    Centrosomal protein 1

Gene names

Name:	Cep110
Synonyms:	Cep1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	2334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in cell cycle progression and cytokinesis. During the late steps of cytokinesis, anchors exocyst and SNARE complexes at the midbody, thereby allowing secretory vesicle-mediated abscission By similarity.
Subunit structure	Interacts with EXOC6 and SNAPIN. Associates with the exocyst complex By similarity. Interacts with HOOK2.
Subcellular location	CentrosomeBy similarity. Note= During cytokinesis, localizes to a ring-like structure at the central midbody By similarity.
Tissue specificity	Highly expressed in liver.
Sequence similarities	Contains 13 LRR (leucine-rich) repeats.
Sequence caution	CAM17906.1 sequence differs from that shown. Reason: Erroneous gene model prediction. CAM17908.1 sequence differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Leucine-rich repeat Repeat
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	centrosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A2AL36-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A2AL36-2)

The sequence of this isoform differs from the canonical sequence as follows:
     552-559: SHMKAQKR → VSKMGNLD
     560-2334: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 2334	2334	Centriolin
Regions
	Repeat	124 – 146	23	LRR 1
	Repeat	147 – 168	22	LRR 2
	Repeat	169 – 194	26	LRR 3
	Repeat	196 – 216	21	LRR 4
	Repeat	217 – 241	25	LRR 5
	Repeat	531 – 555	25	LRR 6
	Repeat	651 – 676	26	LRR 7
	Repeat	814 – 839	26	LRR 8
	Repeat	987 – 1012	26	LRR 9
	Repeat	1649 – 1673	25	LRR 10
	Repeat	1701 – 1725	25	LRR 11
	Repeat	1824 – 1851	28	LRR 12
	Repeat	2076 – 2101	26	LRR 13
	Region	1951 – 2121	171	Required for centrosome localization By similarity
	Region	1985 – 2326	342	Interaction with HOOK2
	Coiled coil	265 – 343	79	Potential
	Coiled coil	1320 – 1632	313	Potential
	Coiled coil	2235 – 2259	25	Potential
	Compositional bias	957 – 960	4	Poly-Lys
	Compositional bias	1239 – 1310	72	Pro-rich
Amino acid modifications
	Modified residue	117	1	Phosphotyrosine By similarity
	Modified residue	286	1	Phosphothreonine By similarity
	Modified residue	1671	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	552 – 559	8	SHMKAQKR → VSKMGNLD in isoform 2.
	Alternative sequence	560 – 2334	1775	Missing in isoform 2.
Experimental info
	Sequence conflict	8	1	R → K in AAH38386. Ref.2
	Sequence conflict	169	1	C → G in BAB32012. Ref.1
	Sequence conflict	1219	1	Missing in BAD21389. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 43C2CDA65A295857	FASTA	2,334	268,880
	10 20 30 40 50 60 MKKGSERRLS KAKMPLSSHF PGPSSLRSSM RSRSLSPLIG SETQPLHPGG QWPAQAELTD 70 80 90 100 110 120 ESTVPLEPQQ RKGAESYVGV RYITEALIKK LTKQDNLALV KSLNLSLSKD GGKKFRYIEN 130 140 150 160 170 180 LEKCVKLEVL NLSYNLIVKI EKVDKLLRLR ELNLSYNKIS KIEGLENMCN LQKLNLAGNE 190 200 210 220 230 240 IEHIPVWFAK KLKSLRVLNL KGNKISSLQD VSKLKPLQDL TSLVLIDNPV VALPHYLQFI 250 260 270 280 290 300 IFHLRSLESL EGQPVTTQDR QEAFERFSLE EIERLEKDLE KKTVETEELK NKQTKFLEEI 310 320 330 340 350 360 KNQDKLNKSL KEEAMLQKQS CEELESDLST KKELLKQKTV ELTRACQKQY ELEQELAFYK 370 380 390 400 410 420 IDAKFEPLNY YPSEYAEIDK YPDESPYIGK SRYKRNMFAT ETYIVSDAQA VQIRKMVPEG 430 440 450 460 470 480 GQLRHEHTPP RVQAPPDLQL EDTEKKISAA QTRLSELHHE IETAEQKVLR ATQEFKQLEE 490 500 510 520 530 540 AIQQKKISEA EKDLLLKQLS GRLQHLNRLR QEALDLEIQM EKQRKEIAEK HEEINTVQLA 550 560 570 580 590 600 TDSLDPKDPK HSHMKAQKRG KEQQLDIMNR QYTQLESRLD EILCRIAKET EEIKDLEQQL 610 620 630 640 650 660 TDGQIAANEA LKKDLEGVIS GLQEYLGTIK GQATQAQNEC RKLQDEKETL LQRLTEVQQE 670 680 690 700 710 720 KEELELIAMD AENMRKELAE LESALQEQHE VNASLQQAQG DLSAYETELE TQLKLKDAET 730 740 750 760 770 780 SQLKQELEKL LRRTQLEQSV LQTELEKERE SLRDALGKAQ SSEEKQQENN ELRTQLKQLQ 790 800 810 820 830 840 DDNSLLKKQL KEFQNHLNHV VDGLIHPEEV AARVDELRKR LKLGAGEMRI HSPSDVLGKS 850 860 870 880 890 900 LADLQKQFSE ILARSQWEKE EAQVRERKLH EEMALQQEKL ANGQEEFRQA CERALEARIK 910 920 930 940 950 960 FDKRQHNARI QQLENEIHYL QENLKSMEKI QGLTDLQLQE ADEEKERILA QLQELEKKKK 970 980 990 1000 1010 1020 REDARSQEQF LGLDEELKSL KKAVAASDKL AAAELTIAKD QLKSLHGTVV RINQERAEEL 1030 1040 1050 1060 1070 1080 QEAERFSREA MQAAKDLSRA EAEIELLQHL LREREGQFRD EMENADLGAK GANSQLLEIE 1090 1100 1110 1120 1130 1140 ALNEAMAKQR AEITRLRDVL NLTGAGTKGG IENVLEEIAE LRHAVSAQNE YISSMADPFR 1150 1160 1170 1180 1190 1200 RQGWWYFMPP APSSKVSSHS SQATKDSGLG LKYTASTPLR KPQPGQQEEK DSSGPLPASG 1210 1220 1230 1240 1250 1260 YWVYSPIRST LHKSFSKRED ADSGGDSQEE SGLDDQEEPP FVPPPGYIMY TVLPDGSPVP 1270 1280 1290 1300 1310 1320 QGVALYAPSP PLPNSSHPLT PGTVVYGPPP AGAPIIYGPP PANFAVPLVP AGVQHCNIPE 1330 1340 1350 1360 1370 1380 HHNLENEVSR LEDIMQHLKS KQREERRQKA STQHSEEEVD GLHRDIDDLL QEKKELELEV 1390 1400 1410 1420 1430 1440 EELHRTIERH QQRKDFIDGH VENLMTELEI EKSLKHHEDI VDEIECLEKT LLKRRSELRE 1450 1460 1470 1480 1490 1500 ADRLLAEAEN ELACTKEKTK SAVEKFTDAK RNLLQTESDA EALEKRAQET ALNLVKAEQQ 1510 1520 1530 1540 1550 1560 LRLLQADAED LEQHKIKQEE ILKEINKVVA AKDADFQCLN EKKEKLTEEL QSLQRDIKAA 1570 1580 1590 1600 1610 1620 QHSEDHHLQV LRESETLLQA KRAELETLKS QVTSQQQELA VLDSELGHRR EELLLLQDSL 1630 1640 1650 1660 1670 1680 AQAKADLQEA LTLGETEVAE KCSHIREVKS LLEELSFQKG ELNVHISEKK TQLALIQQEM 1690 1700 1710 1720 1730 1740 EKEEKNLQVV LQQLSRHKTE LKNVADILQL ETSELQGLKL QHDQKVVELE KAQVDVLEEK 1750 1760 1770 1780 1790 1800 LELENLQQAT QQQRRELERQ RQLLERDRRE TERVRAESQA LQSCVECLSK EKEDLQGQCE 1810 1820 1830 1840 1850 1860 SWEKKSSHAQ RVLAATEESN KMEQSNLGKL ELSVRKLRQE LEQLSQDKLA LHSEVAEVQQ 1870 1880 1890 1900 1910 1920 QLQGKQEAIN SLQEELDSTQ DHLDLAKQDL IHTTKCQNEL LNEQTQLQED ISKWMARLES 1930 1940 1950 1960 1970 1980 CQKETETKEQ QVQQLQDEIR ESKLRLDQQE MMFQKLQKER EREEQKFEAG KVTLEQQQRQ 1990 2000 2010 2020 2030 2040 LEKELTDQKS RLKQLLTDVS AAEGRLGTLQ EEERRIEGLE RMLSQAKQQL SEREQQLMAK 2050 2060 2070 2080 2090 2100 SGELLALQKE ADDMRADFSL LRNQFLTERK KAEKQVAGLK EALKIQRSQL EKNLLEQKQE 2110 2120 2130 2140 2150 2160 NSCMQKEMAT IELVAQDNHE RARRLMKELS QMQQEYLELK KQVANQKDLE RRQMEVSDAM 2170 2180 2190 2200 2210 2220 RTLKSEVKDE IRTSLRNLNQ FLPELPADLA SILERNENLR ELESLKENFP FTTKERIFEE 2230 2240 2250 2260 2270 2280 KSNFPQVHIM DEHWRGEALR QRLRRHEDQL KAQLRHCMSK QAEVLIKGKQ QTEGTLHSLR 2290 2300 2310 2320 2330 RQVDALGELV TSTSTDSASS PSLPSLVEDS QHGHSQSSFQ VLQVPLEEPN SYRH
	Isoform 2 [UniParc]. Checksum: 2579D4875C1CFC46		559	64,469
	10 20 30 40 50 60 MKKGSERRLS KAKMPLSSHF PGPSSLRSSM RSRSLSPLIG SETQPLHPGG QWPAQAELTD 70 80 90 100 110 120 ESTVPLEPQQ RKGAESYVGV RYITEALIKK LTKQDNLALV KSLNLSLSKD GGKKFRYIEN 130 140 150 160 170 180 LEKCVKLEVL NLSYNLIVKI EKVDKLLRLR ELNLSYNKIS KIEGLENMCN LQKLNLAGNE 190 200 210 220 230 240 IEHIPVWFAK KLKSLRVLNL KGNKISSLQD VSKLKPLQDL TSLVLIDNPV VALPHYLQFI 250 260 270 280 290 300 IFHLRSLESL EGQPVTTQDR QEAFERFSLE EIERLEKDLE KKTVETEELK NKQTKFLEEI 310 320 330 340 350 360 KNQDKLNKSL KEEAMLQKQS CEELESDLST KKELLKQKTV ELTRACQKQY ELEQELAFYK 370 380 390 400 410 420 IDAKFEPLNY YPSEYAEIDK YPDESPYIGK SRYKRNMFAT ETYIVSDAQA VQIRKMVPEG 430 440 450 460 470 480 GQLRHEHTPP RVQAPPDLQL EDTEKKISAA QTRLSELHHE IETAEQKVLR ATQEFKQLEE 490 500 510 520 530 540 AIQQKKISEA EKDLLLKQLS GRLQHLNRLR QEALDLEIQM EKQRKEIAEK HEEINTVQLA 550 TDSLDPKDPK HVSKMGNLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Testis, Thymus and Wolffian duct.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: Czech II. Tissue: Mammary tumor.
[3]	The mouse genome sequencing consortium Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H. DNA Res. 11:127-135(2004) [PubMed: 15449545] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1160-2334 (ISOFORM 1). Tissue: Fetal brain.
[5]	"FGFR1 is fused to the centrosome-associated protein CEP110 in the 8p12 stem cell myeloproliferative disorder with t(8;9)(p12;q33)." Guasch G., Mack G.J., Popovici C., Dastugue N., Birnbaum D., Rattner J.B., Pebusque M.-J. Blood 95:1788-1796(2000) [PubMed: 10688839] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Hook2 localizes to the centrosome, binds directly to centriolin/CEP110 and contributes to centrosomal function." Szebenyi G., Hall B., Yu R., Hashim A.I., Kraemer H. Traffic 8:32-46(2007) [PubMed: 17140400] [Abstract] Cited for: INTERACTION WITH HOOK2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK020148 mRNA. Translation: BAB32012.3. AK031338 mRNA. Translation: BAC27353.1. AK041715 mRNA. Translation: BAC31039.1. BC038386 mRNA. Translation: AAH38386.1. AL773523, AL845534 Genomic DNA. Translation: CAM16866.1. AL773523, AL845534 Genomic DNA. Translation: CAM16867.1. AL773523, AL845534 Genomic DNA. Translation: CAM16870.1. AL845534, AL773523 Genomic DNA. Translation: CAM17903.1. AL845534 Genomic DNA. Translation: CAM17904.1. AL845534, AL773523 Genomic DNA. Translation: CAM17906.1. Sequence problems. AL845534, AL773523 Genomic DNA. Translation: CAM17908.1. Sequence problems. AK131139 mRNA. Translation: BAD21389.1.
UniGene	Mm.231332
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000057110. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:1889576. Cep110.
Phylogenomic databases
HOVERGEN	A2AL36.
Gene expression databases
CleanEx	MM_CEP110.
Family and domain databases
InterPro	IPR001611. LRR. [Graphical view]
Pfam	PF00560. LRR_1. 4 hits. [Graphical view]
PRINTS	PR00019. LEURICHRPT.
ProDom	A2AL36. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

CP110_MOUSE

Accession

Primary (citable) accession number: A2AL36
Secondary accession number(s): A0A4W6 , A0A4X1, A2AL37, A2AL40, Q6KAR8, Q8CHX3, Q9CRM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	July 22, 2008
This is version 13 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)