Reviewed, UniProtKB/Swiss-Prot A2ZC67 (ASP1_ORYSI)

Names and origin

Protein names

Recommended name:
    Aspartic proteinase Asp1
      Short name=OsAsp1
      Short name=OSAP1
    EC=3.4.23.-
Alternative name(s):
    Nucellin-like protein

Gene names

Name:	ASP1
ORF Names:	OsI_034160

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Possesses protease activity in vitro.
Tissue specificity	Expressed in pollen, nucellus, ovary wall, shoot and root meristem, coleoptiles of immature seeds, and somatic embryos.
Developmental stage	Abundantly expressed in zygotic embryos 1 and 2 days after fertilization.
Sequence similarities	Belongs to the peptidase A1 family.
Sequence caution	The sequence EAY80201.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Aspartyl protease Hydrolase Protease
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Propeptide	24 – 46	23	Removed in mature form Potential
	Chain	47 – 410	364	Aspartic proteinase Asp1
Sites
	Active site	56	1	By similarity
	Active site	257	1	By similarity
Experimental info
	Sequence conflict	45	1	G → S in AAK81698 and AAK81699. Ref.1
	Sequence conflict	239	1	Q → H in AAK81698 and AAK81699. Ref.1
	Sequence conflict	244 – 245	2	SK → KQS in AAK81699. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A2ZC67-1 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: AAC9775F3234FB9A	FASTA	410	44,961
	10 20 30 40 50 60 MTARLALLAS LLLLLQLVPP SSAVVLELHG NVYPIGHFFV TMNIGDPAKP YFLDIDTGST 70 80 90 100 110 120 LTWLQCDYPC INCNKVPHGL YKPELKYAVK CTEQRCADLY ADLRKPMKCG PKNQCHYGIQ 130 140 150 160 170 180 YVGGSSIGVL IVDSFSLPAS NGTNPTSIAF GCGYNQGKNN HNVPTPVNGI LGLGRGKVTL 190 200 210 220 230 240 LSQLKSQGVI TKHVLGHCIS SKGKGFLFFG DAKVPTSGVT WSPMNREHKH YSPRQGTLQF 250 260 270 280 290 300 NSNSKPISAA PMEVIFDSGA TYTYFALQPY HATLSVVKST LSKECKFLTE VKEKDRALTV 310 320 330 340 350 360 CWKGKDKIRT IDEVKKCFRS LSLKFADGDK KATLEIPPEH YLIISQEGHV CLGILDGSKE 370 380 390 400 410 HPSLAGTNLI GGITMLDQMV IYDSERSLLG WVNYQCDRIP RSASAITSRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The rice nucellin gene ortholog OsAsp1 encodes an active aspartic protease without a plant-specific insert and is strongly expressed in early embryo." Bi X., Khush G.S., Bennett J. Plant Cell Physiol. 46:87-98(2005) [PubMed: 15659452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. IR64.
[2]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY043216 Genomic DNA. Translation: AAK81698.1. AY043217 mRNA. Translation: AAK81699.1. CM000136 Genomic DNA. Translation: EAY80201.1. Sequence problems.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	A01.073.
Organism-specific databases
Gramene	Q6YNY7.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProDom	A2ZC67. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ASP1_ORYSI

Accession

Primary (citable) accession number: A2ZC67
Secondary accession number(s): Q53NF8 , Q6YNY7, Q6YNY8, Q71SA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	July 22, 2008
This is version 13 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)