Reviewed, UniProtKB/Swiss-Prot O96623 (ARPC2_DICDI)

Names and origin

Protein names

Recommended name:
    Actin-related protein 2/3 complex subunit 2
Alternative name(s):
    Arp2/3 complex 34 kDa subunit
      Short name=p34-ARC

Gene names

Name:	arcB
Synonyms:	Arc35, arpE
ORF Names:	DDB_0214935

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. The Arp2/3 complex is involved in organizing the actin system in cell motility and chemotaxis, in phagocytosis and macropinocytosis, at late steps of endosome processing, and in mitosis. In concert with a group of other proteins, the Arp2/3 complex plays a general role in the rapid activation and adaptation of the actin system to its multiple functions.
Subunit structure	Component of the Arp2/3 complex composed of arpB/Arp2, arpC/Arp3, arcA/p41-arc, arcB/p34-arc, arcC/p21-arc, arcD/p20-arc and arcE/p16-arc.
Subcellular location	Cytoplasm › cytoskeleton. Cell projection. Cytoplasm › cytosol. Cytoplasm › cell cortex.
Sequence similarities	Belongs to the ARPC2 family.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm Cytoskeleton
Ligand	Actin-binding
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 293	293	Actin-related protein 2/3 complex subunit 2

Sequences

	Sequence		Length	Mass (Da)
	O96623-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: A3220726D708CBD8	FASTA	293	32,932
	10 20 30 40 50 60 MLLLETHNRI LYDEVISHFE GDRRVNNIFA DFDGVKFNVQ TSDDKSSLMV SVSLHAAADL 70 80 90 100 110 120 LKNGGSALLK SVYGDMLQAK PEGGYDVTLV IQSSFSGNKE ELAKKVSLLK RHLVAAPFLM 130 140 150 160 170 180 VFEGIEAKKP LPEIIAINYR TDETFYLKPQ GDNVIVIFDI AFKDADDVIL SKIFLQSFVD 190 200 210 220 230 240 VRKTISNVPS ITFSQKDPPL ELKGVKGVRA GQANHGFVSF VLFPAHIKKP QESADLIQTF 250 260 270 280 290 RDYLHYHIKC AKGYMHTSMR NRVESLIQVL NRAKPEPVNT VKRTITGKFF KQN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis." Insall R., Mueller-Taubenberger A., Machesky L., Koehler J., Simmeth E., Atkinson S.J., Weber I., Gerisch G. Cell Motil. Cytoskeleton 50:115-128(2001) [PubMed: 11807934] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Identification and isolation of Dictyostelium microtubule-associated protein interactors by tandem affinity purification." Koch K.V., Reinders Y., Ho T.-H., Sickmann A., Graef R. Eur. J. Cell Biol. 85:1079-1090(2006) [PubMed: 16782229] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[4]	"Mutants in the Dictyostelium Arp2/3 complex and chemoattractant-induced actin polymerization." Langridge P.D., Kay R.R. Exp. Cell Res. 313:2563-2574(2007) [PubMed: 17553489] [Abstract] Cited for: FUNCTION, MUTAGENESIS.
[5]	"Vectors for expression of proteins with single or combinatorial fluorescent protein and tandem affinity purification tags in Dictyostelium." Meima M.E., Weening K.E., Schaap P. Protein Expr. Purif. 53:283-288(2007) [PubMed: 17296313] [Abstract] Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF095931 mRNA. Translation: AAC99778.1. AAFI02000047 Genomic DNA. Translation: EAL65986.1.
RefSeq	XP_639348.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3390911.
KEGG	ddi:DDB_0214935.
Organism-specific databases
dictyBase	DDB0214935. arcB.
Family and domain databases
InterPro	IPR007188. P34-arc. [Graphical view]
PANTHER	PTHR12058. P34-arc. 1 hit.
Pfam	PF04045. P34-Arc. 1 hit. [Graphical view]
ProDom	O96623. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ARPC2_DICDI

Accession

Primary (citable) accession number: O96623
Secondary accession number(s): Q54RY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	September 2, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)