Reviewed, UniProtKB/Swiss-Prot O96624 (ARPC3_DICDI)

Names and origin

Protein names

Recommended name:
    Actin-related protein 2/3 complex subunit 3
Alternative name(s):
    Arp2/3 complex 21 kDa subunit
      Short name=p21-ARC

Gene names

Name:	arcC
Synonyms:	Arc18, arpF
ORF Names:	DDB_0201632

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. The Arp2/3 complex is involved in organizing the actin system in cell motility and chemotaxis, in phagocytosis and macropinocytosis, at late steps of endosome processing, and in mitosis. In concert with a group of other proteins, the Arp2/3 complex plays a general role in the rapid activation and adaptation of the actin system to its multiple functions.
Subunit structure	Component of the Arp2/3 complex composed of arpB/Arp2, arpC/Arp3, arcA/p41-arc, arcB/p34-arc, arcC/p21-arc, arcD/p20-arc and arcE/p16-arc.
Subcellular location	Cytoplasm › cytoskeleton. Cell projection. Cytoplasm › cytosol. Cytoplasm › cell cortex.
Sequence similarities	Belongs to the ARPC3 family.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm Cytoskeleton
Ligand	Actin-binding
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 174	174	Actin-related protein 2/3 complex subunit 3

Sequences

	Sequence		Length	Mass (Da)
	O96624-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 82A7E57B0302C3E6	FASTA	174	19,548
	10 20 30 40 50 60 MVYHSQFNDE SAGFRLVGNV PILPLKTTHK GPAPKGDANS VDIIDEALDL FKANILFRNF 70 80 90 100 110 120 EVQGNGDRVL IYLTLYITKC LLKIAPMNKA DAEKALFLIA QEQFSIPGES AFPLGGLVTV 130 140 150 160 170 PNTRDAADTL RQYFTQLRLE LGVRLCQRVY AVDPSKANKW WICFSKRKFL NKAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis." Insall R., Mueller-Taubenberger A., Machesky L., Koehler J., Simmeth E., Atkinson S.J., Weber I., Gerisch G. Cell Motil. Cytoskeleton 50:115-128(2001) [PubMed: 11807934] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Vectors for expression of proteins with single or combinatorial fluorescent protein and tandem affinity purification tags in Dictyostelium." Meima M.E., Weening K.E., Schaap P. Protein Expr. Purif. 53:283-288(2007) [PubMed: 17296313] [Abstract] Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF095932 mRNA. Translation: AAC99779.1. AAFI02000197 Genomic DNA. Translation: EAL60951.1.
RefSeq	XP_629371.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3386028.
KEGG	ddi:DDB_0201632.
Organism-specific databases
dictyBase	DDB0201632. arcC.
Family and domain databases
InterPro	IPR007204. ARP2/3_P21-Arc. [Graphical view]
Gene3D	G3DSA:1.10.1760.10. ARP2/3_P21-Arc. 1 hit.
PANTHER	PTHR12391. P21-Arc. 1 hit.
Pfam	PF04062. P21-Arc. 1 hit. [Graphical view]
PIRSF	PIRSF016315. ARP2/3_P21-Arc. 1 hit.
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ARPC3_DICDI

Accession

Primary (citable) accession number: O96624
Secondary accession number(s): Q54CP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	September 23, 2008
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)