Reviewed, UniProtKB/Swiss-Prot P13021 (CAPZB_DICDI)

Names and origin

Protein names

Recommended name:
    F-actin-capping protein subunit beta
Alternative name(s):
    Aginactin subunit beta
    CAP32

Gene names

Name:	acpA
Synonyms:	abpE
ORF Names:	DDB_0191202

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Subunit structure	Heterodimer of an alpha and a beta subunit.
Subcellular location	Cytoplasm › cytoskeletonBy similarity.
Sequence similarities	Belongs to the F-actin-capping protein beta subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	Actin-binding
Molecular function	Actin capping
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 272	272	F-actin-capping protein subunit beta

Sequences

	Sequence		Length	Mass (Da)
	P13021-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: EB2977B607512CF5	FASTA	272	30,695
	10 20 30 40 50 60 MTEKQLSCCL DLMRRLPPSQ IEDNLAGLLD LVPDLTEDLL SSIDQPLKVA YDAVSKKDYL 70 80 90 100 110 120 LCDYNRDADS YRSPWSNKYD PPLSGACYPS SKLRDIEVQA NEIFEIYLNL YFEGGVSSVY 130 140 150 160 170 180 CWDLDDNFAA VVLMKKTQDQ SKKGQPMRGT WDSIHVVEVK LGKKDKAVYK LTSTVMLSIE 190 200 210 220 230 240 TDNDNTGKVN LAGSLTRQDE KEYTFNEVDT HCVNIGKMVE DMESKLRQTL ETIYFGKTKE 250 260 270 VVNTLRNATG NSELEKRKNL SNQIGSAIGN RG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ca2+-independent F-actin capping proteins. Cap 32/34, a capping protein from Dictyostelium discoideum, does not share sequence homologies with known actin-binding proteins." Hartmann H., Noegel A.A., Eckerskorn C., Rapp S., Schleicher M. J. Biol. Chem. 264:12639-12647(1989) [PubMed: 2663863] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Heterodimeric capping proteins constitute a highly conserved group of actin-binding proteins." Hartmann H., Schleicher M., Noegel A.A. Dev. Genet. 11:369-376(1990) [PubMed: 1710551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"A major agonist-regulated capping activity in Dictyostelium is due to the capping protein, cap32/34." Eddy R.J., Han J., Sauterer R.A., Condeelis J.S. Biochim. Biophys. Acta 1314:247-259(1996) [PubMed: 8982279] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M25131 mRNA. Translation: AAA33175.1. AAFI02000003 Genomic DNA. Translation: EAL73139.1.
PIR	A61042.
RefSeq	XP_647630.1.
3D structure databases
HSSP	HSSP built from PDB template 1IZN based on UniProtKB P14315.
ModBase	Search...
Genome annotation databases
GeneID	3398229.
KEGG	ddi:DDB_0191202.
Organism-specific databases
dictyBase	DDB0191202. acpA.
Family and domain databases
InterPro	IPR001698. Factin_cap_beta. [Graphical view]
PANTHER	PTHR10619. Factin_cap_beta. 1 hit.
Pfam	PF01115. F_actin_cap_B. 1 hit. [Graphical view]
PRINTS	PR00192. FACTINCAPB.
ProDom	PD008617. Factin_cap_beta. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00231. F_ACTIN_CAPPING_BETA. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

CAPZB_DICDI

Accession

Primary (citable) accession number: P13021
Secondary accession number(s): Q55FA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	July 22, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)