Reviewed, UniProtKB/Swiss-Prot P34121 (COAC_DICDI)

Names and origin

Protein names

Recommended name:
    Coactosin
Alternative name(s):
    Cytoskeletal protein p17
    Cyclic AMP-regulated protein p16

Gene names

Name:	coaA
Synonyms:	p17
ORF Names:	DDB_0215369

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to F-actin in a calcium independent manner. Binds to the filaments along their length.
Subcellular location	Cytoplasm › cytoskeleton.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the actin-binding proteins ADF family. Coactosin subfamily. Contains 1 ADF-H domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	Actin-binding
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 146	146	Coactosin
Regions
	Domain	1 – 132	132	ADF-H

Sequences

	Sequence		Length	Mass (Da)
	P34121-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: EE7AE2432D8FE1D9	FASTA	146	16,004
	10 20 30 40 50 60 MADVSSTELK AAYDEVLADS NDTNWCLFKY EGKNKIVLSG KGSGGFAELA QEINQPSERL 70 80 90 100 110 120 YAYLRVVSGD DESKRSKFVF ISWCGEEVGP LAKANVSVHK ASVKQVIKNI GVEVHYTVAD 130 140 DLNEEELMTK VRKSSGADYS GNKSTN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Coactosin, a 17 kDa F-actin binding protein from Dictyostelium discoideum." de Hostos E.L., Bradtke B., Lottspeich F., Gerisch G. Cell Motil. Cytoskeleton 26:181-191(1993) [PubMed: 8293475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 128-133. Strain: AX2.
[2]	"Cloning and characterization of a Dictyostelium discoideum cDNA coding for p16, a novel cyclic AMP-regulated protein with a nuclear localization signal." Sastry R., Abbruzzi G., Tauber A.I. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AX3.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"Identification of novel centrosomal proteins in Dictyostelium discoideum by comparative proteomic approaches." Reinders Y., Schulz I., Graef R., Sickmann A. J. Proteome Res. 5:589-598(2006) [PubMed: 16512674] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[5]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X61797 mRNA. Translation: CAA43899.1. M92992 mRNA. Translation: AAA33174.1. AAFI02000224 Genomic DNA. Translation: EAL60448.1.
PIR	S17111.
RefSeq	XP_628885.1.
3D structure databases
ModBase	Search...
2-D gel databases
SWISS-2DPAGE	P34121.
Genome annotation databases
GeneID	3385539.
KEGG	ddi:DDB_0215369.
Organism-specific databases
dictyBase	DDB0215369. coaA.
Family and domain databases
InterPro	IPR002108. Cofilin_actin_bd. [Graphical view]
Pfam	PF00241. Cofilin_ADF. 1 hit. [Graphical view]
SMART	SM00102. ADF. 1 hit. [Graphical view]
PROSITE	PS51263. ADF_H. 1 hit. [Graphical view]
ProDom	P34121. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

COAC_DICDI

Accession

Primary (citable) accession number: P34121
Secondary accession number(s): Q54B31

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	September 23, 2008
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)