Reviewed, UniProtKB/Swiss-Prot Q02720 (CSK2A_DICDI)

Names and origin

Protein names

Recommended name:
    Casein kinase II subunit alpha
      Short name=CK II subunit alpha
    EC=2.7.11.1

Gene names

Name:	casK
ORF Names:	DDB_0185025

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Tetramer of two alpha and two beta chains Probable.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 337	337	Casein kinase II subunit alpha
Regions
	Domain	47 – 332	286	Protein kinase
	Nucleotide binding	53 – 61	9	ATP By similarity
Sites
	Active site	164	1	Proton acceptor By similarity
	Binding site	76	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02720-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 49DE7A7F39E1ED12	FASTA	337	39,898
	10 20 30 40 50 60 MNHSSKKNKN RILRNKARIY CDVNLHKPKE YWNYEALNVK WETQDDYEII RKIGRGKYSE 70 80 90 100 110 120 VFEGANIKNN EKCVIKVLKP VKKKKIKREI KILQNLCGGP NIITLYDVVR DPQSKTPSLI 130 140 150 160 170 180 FEYINNTDFK HLSPTLTDFD VRYYIRELLH ALDFCHSNGI MHRDVKPSNV MIDHQKRKLY 190 200 210 220 230 240 LIDWGLAEFY HPNQDYNVRV ASRPYKGPEL LVDMEDYDYS LDMWSLGCMF AGMLFQKDPF 250 260 270 280 290 300 FHGHDNIDQL VKIVKILGTE EFYAYLDKYG IVVDHTILSI IGKHPKKPWS RFITKENQHL 310 320 330 AVPEAIDFLE KLLRYDPAER LTTREAMEHP YFKPLSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of casein kinase II alpha subunit from Dictyostelium discoideum and its expression in the life cycle." Kikkawa U., Mann S.K., Firtel R.A., Hunter T. Mol. Cell. Biol. 12:5711-5723(1992) [PubMed: 1448100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L05535 mRNA. Translation: AAA33180.1. AAFI02000019 Genomic DNA. Translation: EAL68944.1.
PIR	A45038.
RefSeq	XP_642893.1.
3D structure databases
HSSP	HSSP built from PDB template 1M2R based on UniProtKB P28523.
SMR	Q02720. Positions 15-333.
ModBase	Search...
Genome annotation databases
GeneID	3394126.
KEGG	ddi:DDB_0185025.
Organism-specific databases
dictyBase	DDB0185025. casK.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

CSK2A_DICDI

Accession

Primary (citable) accession number: Q02720
Secondary accession number(s): Q550N9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	September 23, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)