Reviewed, UniProtKB/Swiss-Prot P18624 (EF1A_DICDI)

Names and origin

Protein names

Recommended name:
    Elongation factor 1-alpha
      Short name=EF-1-alpha
Alternative name(s):
    50 kDa actin-binding protein
    ABP-50

Gene names

Name:	eef1a1
Synonyms:	efaa1, efaAI
ORF Names:	DDB_0191135
AND
Name:	eef1a2
Synonyms:	efaa2, efaAII
ORF Names:	DDB_0191134

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is also an abundant actin filament bundling protein.
Subunit structure	Binds to actin.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	Actin-binding GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 453	453	Elongation factor 1-alpha
Regions
	Nucleotide binding	14 – 21	8	GTP By similarity
	Nucleotide binding	91 – 95	5	GTP By similarity
	Nucleotide binding	153 – 156	4	GTP By similarity
Experimental info
	Sequence conflict	1 – 3	3	MGK → MEFPES in CAA39443. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P18624-1 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 5D608E4B722CE003	FASTA	453	49,662
	10 20 30 40 50 60 MGKEKTHINI VVIGHVDAGK STTTGHLIYK CGGIDKRVIE KYEKEASEMG KQSFKYAWVM 70 80 90 100 110 120 DKLKAERERG ITIDIALWKF ETSKYYFTII DAPGHRDFIK NMITGTSQAD CAVLVIASPT 130 140 150 160 170 180 GEFEAGIAKN GQTREHALLA YTLGVKQMIV AINKMDEKST NYSQARYDEI VKEVSSFIKK 190 200 210 220 230 240 IGYNPEKVAF VPISGWNGDN MLERSDKMEW YKGPTLLEAL DAIVEPKRPH DKPLRIPLQD 250 260 270 280 290 300 VYKIGGIGTV PVGRVETGII KPGMVVTFAP AGLSTEVKSV EMHHEQLPEA RPGDNVGFNV 310 320 330 340 350 360 KNVSVKEIKR GMVAGDSKND PPQETEKFVA QVIVLNHPGQ IHAGYSPVLD CHTAHIACKF 370 380 390 400 410 420 TEIVDKVDRR TGAVVAKEGT AAVVLKNGDA AMVELTPSRP MCVESFTEYP PLGRFAVRDM 430 440 450 RQTVAVGVIK STVKKAPGKA GDKKGAAAPS KKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of an actin-binding protein from Dictyostelium as elongation factor 1a." Yang F., Demma M., Warren V., Dharmawardhane S., Condeelis J. Nature 347:494-496(1990) [PubMed: 2215665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, INTERACTION WITH ACTIN. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55972 mRNA. Translation: CAA39442.1. X55973 mRNA. Translation: CAA39443.1. AAFI02000005 Genomic DNA. Translation: EAL71917.1. AAFI02000005 Genomic DNA. Translation: EAL71918.1.
PIR	S11665.
RefSeq	XP_645839.1. XP_645978.1.
3D structure databases
HSSP	HSSP built from PDB template 1G7C based on UniProtKB P02994.
SMR	P18624. Positions 7-437.
ModBase	Search...
Genome annotation databases
GeneID	3397701. 3397702.
KEGG	ddi:DDB_0191134. ddi:DDB_0191135.
Organism-specific databases
dictyBase	DDB0191135. eef1a1. DDB0191134. eef1a2.
Family and domain databases
InterPro	IPR000795. ProtSyn_GTP_bd. IPR004539. Transl_elong_EF1A_euk/arc. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004160. Transl_elong_EFTu/EF1A_C. [Graphical view]
Pfam	PF00009. GTP_EFTU. 2 hits. PF03144. GTP_EFTU_D2. 2 hits. PF03143. GTP_EFTU_D3. 2 hits. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
TIGRFAMs	TIGR00483. EF-1_alpha. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProDom	P18624. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

EF1A_DICDI

Accession

Primary (citable) accession number: P18624
Secondary accession number(s): Q55E03

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	December 4, 2007
Last modified:	July 22, 2008
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)