Reviewed, UniProtKB/Swiss-Prot Q94469 (G3P_DICDI)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name(s)=GAPDH
    EC=1.2.1.12

Gene names

Name:	gpdA
ORF Names:	DDB_0185087

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 335	335	Glyceraldehyde-3-phosphate dehydrogenase
Regions
	Nucleotide binding	12 – 13	2	NAD By similarity
	Region	150 – 152	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	210 – 211	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	151	1	Nucleophile By similarity
	Binding site	34	1	NAD By similarity
	Binding site	79	1	NAD; via carbonyl oxygen By similarity
	Binding site	181	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	233	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	316	1	NAD By similarity
	Site	178	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q94469-1 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 58CCC779D16CDF5E	FASTA	335	36,552
	10 20 30 40 50 60 MVVPIGINGF GRIGRLVLRA SLENPECRVL AINEPFMDVK YMVYMFKYDS THGRFKGTVE 70 80 90 100 110 120 DINGEFVVNG NKIHVFAEKD PANIKWSSVG AEYIVESTGL FLSTEKAGVH LKGGAKKVVI 130 140 150 160 170 180 SAPSTDAPMY VMGVNEETYE SSHDVISNAS CTTNCLAPLA KIIHENFGIV EGLMTTVHAI 190 200 210 220 230 240 TATQKTVDGP SGKDWRAGRS ALSNIIPAST GAAKAVGKVL PALNGKLTGM SFRVPNCDVS 250 260 270 280 290 300 VVDLTVRLEK KATYEEIKKV MKAASESDKY KRYIGYTEDE VVSTDFIGDT HSSIFDAHAG 310 320 330 IALNDNFVKL VSWYDNEMGY STRVIDLLVY ISKKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Evidence for the Heterolobosea from phylogenetic analysis of genes encoding glyceraldehyde-3-phosphate dehydrogenase." Roger A.J., Smith M.W., Doolittle R.F., Doolittle W.F. J. Eukaryot. Microbiol. 43:475-485(1996) [PubMed: 8976605] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 14-312. Strain: AX4.
[4]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC116305 Genomic DNA. Translation: AAO52263.1. AAFI02000013 Genomic DNA. Translation: EAL69857.1. U55243 Genomic DNA. Translation: AAC47285.1.
RefSeq	XP_643857.1.
3D structure databases
HSSP	HSSP built from PDB template 1CRW based on UniProtKB P56649.
SMR	Q94469. Positions 1-299.
ModBase	Search...
Genome annotation databases
GeneID	3394906.
KEGG	ddi:DDB_0185087.
Organism-specific databases
dictyBase	DDB0185087. gpdA.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 2 hits.
Pfam	PF02800. Gp_dh_C. 2 hits. PF00044. Gp_dh_N. 2 hits. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProDom	Q94469. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

G3P_DICDI

Accession

Primary (citable) accession number: Q94469
Secondary accession number(s): Q553V4, Q869Y9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	December 4, 2007
Last modified:	July 22, 2008
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)