Reviewed, UniProtKB/Swiss-Prot P13651 (IF5A_DICDI)

Names and origin

Protein names

Recommended name:
    Eukaryotic translation initiation factor 5A
      Short name=eIF-5A
Alternative name(s):
    eIF-4D

Gene names

Name:	eif5a
Synonyms:	eifE
ORF Names:	DDB_0191442

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.
Post-translational modification	eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
Sequence similarities	Belongs to the eIF-5A family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Initiation factor
PTM	Hypusine
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 159	159	Eukaryotic translation initiation factor 5A
Amino acid modifications
	Modified residue	55	1	Hypusine

Sequences

	Sequence		Length	Mass (Da)
	P13651-1 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 73C0300E5403EEAA	FASTA	159	17,090
	10 20 30 40 50 60 MSDNEALDVE DYAQAGSGAS LTFPIQCSAL RKNGFVVIKG FPCKIVDMST SKTGKHGHAK 70 80 90 100 110 120 VNITAIDIFT GKKYEEICPS THNIDVPNVS RKEYTVMDVQ DGYLSLLDAG GEVKEDLALP 130 140 150 EDDIGKEITQ MLKEGKEPLV SVISALGKEG VVSVKVSNN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA and derived amino acid sequence of the hypusine containing protein from Dictyostelium discoideum." Sandholzer U., Centea-Intemann M., Noegel A.A., Lottspeich F. FEBS Lett. 246:94-100(1989) [PubMed: 2540042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 33-39; 53-72 AND 93-140, HYPUSINE AT LYS-55. Strain: AX3.
[2]	Lottspeich F. Submitted (OCT-1989) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 12-16.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X14970 mRNA. Translation: CAA33095.1. Different initiation. AAFI02000073 Genomic DNA. Translation: EAL64894.1.
PIR	FIDOA. S03886.
RefSeq	XP_639978.1.
3D structure databases
HSSP	HSSP built from PDB template 1IZ6 based on UniProtKB O50089.
ModBase	Search...
Genome annotation databases
GeneID	3391102.
KEGG	ddi:DDB_0191442.
Organism-specific databases
dictyBase	DDB0191442. eif5a.
Family and domain databases
InterPro	IPR001884. EIF5A_hypusine. IPR005824. KOW. IPR012340. NA-bd_OB-fold. IPR014722. Transl_SH3_like_sub. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 2 hits. G3DSA:2.30.30.30. Ribosomal_L2. 2 hits.
PANTHER	PTHR11673. EIF5A_hypusine. 2 hits.
Pfam	PF01287. eIF-5a. 2 hits. PF00467. KOW. 2 hits. [Graphical view]
PIRSF	PIRSF003025. eIF5A. 1 hit.
TIGRFAMs	TIGR00037. eIF_5A. 1 hit.
PROSITE	PS00302. IF5A_HYPUSINE. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

IF5A_DICDI

Accession

Primary (citable) accession number: P13651
Secondary accession number(s): Q54NT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	December 4, 2007
Last modified:	September 23, 2008
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)