Reviewed,
UniProtKB/Swiss-Prot O02626 (MADD_CAEEL)
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December 16, 2008.
Version 56.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: MAP kinase-activating death domain protein Alternative name(s): Regulator of presynaptic activity aex-3 Aboc, expulsion defective protein 3 | ||||
| Gene names |
| ||||
| Organism | Caenorhabditis elegans [Complete proteome] | ||||
| Taxonomic identifier | 6239 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Protein attributes
| Sequence length | 1409 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Plays a novel and significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Plays an important regulatory role in physiological cell death, caspase-mediated apoptosis By similarity. Converts GDP-bound inactive form of rab-3 and cab-1 to the GTP-bound active forms. Novel regulator of presynaptic activity that interacts with rab-3 to regulate synaptic vesicle release. Is also a regulator of the cab-1 synaptic transmission pathway. |
| Subunit structure | Interacts with cab-1. Ref.3 |
| Subcellular location | Membrane; Multi-pass membrane proteinPotential. |
| Tissue specificity | Expressed in nearly all neurons. Ref.1 |
| Miscellaneous | Worms lacking aex-3 exhibit pleiotropic behavioral defects that are suggestive of reduced synaptic transmission. |
| Sequence similarities | Belongs to the MADD family. Contains 1 dDENN domain. Contains 1 death domain. Contains 1 DENN domain. Contains 1 uDENN domain. |
Ontologies
Keywords | |
|---|---|
| Biological process | Apoptosis |
| Cellular component | Membrane |
| Coding sequence diversity | Alternative splicing |
| Domain | Transmembrane |
| Molecular function | Guanine-nucleotide releasing factor |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | apoptosis Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform a (identifier: O02626-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform b (identifier: O02626-2) The sequence of this isoform differs from the canonical sequence as follows: 1385-1409: DQICYAVLCVFSLAAAGHKKEEHSK → VDIAWAMHRVFSVQFAISCQKDTN | ||||||
| Notes: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1409 | 1409 | MAP kinase-activating death domain protein | PRO_0000064469 | |||||
Regions | |||||||||
| Transmembrane | 298 – 318 | 21 | Potential | ||||||
| Transmembrane | 324 – 344 | 21 | Potential | ||||||
| Transmembrane | 1380 – 1400 | 21 | Potential | ||||||
| Domain | 9 – 118 | 110 | uDENN | ||||||
| Domain | 141 – 364 | 224 | DENN | ||||||
| Domain | 419 – 489 | 71 | dDENN | ||||||
| Domain | 1109 – 1184 | 76 | Death | ||||||
| Compositional bias | 604 – 632 | 29 | Ser-rich | ||||||
| Compositional bias | 962 – 1069 | 108 | Pro-rich | ||||||
Natural variations | |||||||||
| Alternative sequence | 1385 – 1409 | 25 | DQICY…EEHSK → VDIAWAMHRVFSVQFAISCQ KDTN in isoform b. | VSP_015555 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "aex-3 encodes a novel regulator of presynaptic activity in C. elegans." Iwasaki K., Staunton J., Saifee O., Nonet M., Thomas J.H. Neuron 18:613-622(1997) [PubMed: 9136770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY. Strain: Bristol N2. |
| [2] | "Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2. |
| [3] | "The rab3 GDP/GTP exchange factor homolog AEX-3 has a dual function in synaptic transmission." Iwasaki K., Toyonaga R. EMBO J. 19:4806-4816(2000) [PubMed: 10970871] [Abstract] Cited for: FUNCTION, INTERACTION WITH CAB-1. |
Cross-references
Sequence databases | |
|---|---|
| U93842 mRNA. Translation: AAB52421.1. U49945 Genomic DNA. Translation: AAC47926.1. U49945 Genomic DNA. Translation: AAM51509.1. | |
| PIR | T37188. |
| RefSeq | NP_741710.1. |
| UniGene | Cel.18148 |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| Ensembl | C02H7.3. Caenorhabditis elegans. [Contig view] |
| GeneID | 180420. |
Organism-specific databases | |
| WormBase | WBGene00000086. aex-3. |
| WormPep | C02H7.3a. CE16806. [WorfDB] C02H7.3b. CE30850. [WorfDB] |
Gene expression databases | |
| ArrayExpress | O02626. |
Family and domain databases | |
| InterPro | IPR005112. dDENN. IPR000488. Death. IPR001194. DENN. IPR005113. uDENN. [Graphical view] |
| Pfam | PF03455. dDENN. 1 hit. PF02141. DENN. 1 hit. PF03456. uDENN. 1 hit. [Graphical view] |
| SMART | SM00801. dDENN. 1 hit. SM00799. DENN. 1 hit. SM00800. uDENN. 1 hit. [Graphical view] |
| PROSITE | PS50947. DDENN. 1 hit. PS50017. DEATH_DOMAIN. False negative. PS50211. DENN. 1 hit. PS50946. UDENN. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 909294. |
Entry information
| Entry name | MADD_CAEEL | ||||||||
| Accession | Primary (citable) accession number: O02626 Secondary accession number(s): Q27467, Q8MQF4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Caenorhabditis annotation project | ||||||||
Relevant documents
| Caenorhabditis elegans Caenorhabditis elegans: entries, gene names and cross-references to WormPep |
| SIMILARITY comments Index of protein domains and families |
