Reviewed, UniProtKB/Swiss-Prot O04197 (COI1_ARATH)

Names and origin

Protein names

Coronatine-insensitive protein 1
Also known as:
     F-box/LRR-repeat protein 2
     AtFBL2
     AtCOI1
     COI-1

Gene names

Name:	COI1
Synonyms:	FBL2
Ordered Locus Names:	At2g39940
ORF Names:	T28M21.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein existence

Evidence at protein level.

General annotation (Comments)

Function	Required for jasmonate-regulated plant fertility and defense processes, and for coronatine and/or other elicitors perceptions/responses. Seems to not be required for meiosis. Required for the regulation of some genes induced by wounding, but not for all. Component of SCF(COI1) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins (probably including the ribulose bisphosphate carboxylase small chain 1B RBCS-1B and the histone deacetylase HDA6). These SCF complexes play crucial roles in regulating response to jasmonate, and their interactions with the COP9 signalosome (CSN) appear to be important for their activity. Involved in the regulation of plant gene expression during plant-pathogen interactions with Pseudomonas syringae and Alternaria brassicicola.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Component of SCF(COI1) E3 ubiquitin ligase complexes at least composed of ASK1 or ASK2, CUL1, RBX1A or RBX1B and COI1. Interacts with ASK1 and ASK2, but separately. Interacts with RBCS-1B and HDA6. SCF complexes interact with the COP9 signalosome (CSN).
Domain	The F-box domain is essential for the formation of SFC(COI1) complexes. The Leu-rich domain is involved in the interactions with RBCS-1B and RPD3B.
Miscellaneous	Mutants coi1-1 to coi1-14 are male sterile, insensitive to MeJA and coronatine, and exhibit enhanced resistance to Pseudomonas syringae atropurpurea (coronatine producing strain). Mutant coi1-16 has reduced sensitivity to jasmonate, but is male fertile when grown below 22 degrees Celsius and male sterile otherwise.
Sequence similarities	Contains 1 F-box domain. Contains 16 LRR (leucine-rich) repeats.

Ontologies

Keywords
Biological process	Plant defense Ubl conjugation pathway
Domain	Leucine-rich repeat Repeat
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	jasmonic acid mediated signaling pathway Ref.1 Traceable author statement. Source: TAIR negative regulation of defense response Inferred from mutant phenotype. Source: TAIR
Molecular function	protein binding Ref.9 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CUL1	Q94AH6	2	EBI-401159,EBI-532411
HDA6	Q9FML2	3	EBI-401159,EBI-639608
RBCS-3B	P10798	2	EBI-401159,EBI-639640
SKP1A	Q39255	6	EBI-401159,EBI-532357
SKP1B	Q9FHW7	5	EBI-401159,EBI-604076

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 592	592	Coronatine-insensitive protein 1
Regions
	Domain	16 – 57	42	F-box
	Repeat	103 – 120	18	LRR 1
	Repeat	121 – 154	34	LRR 2
	Repeat	155 – 182	28	LRR 3
	Repeat	183 – 210	28	LRR 4
	Repeat	211 – 236	26	LRR 5
	Repeat	237 – 264	28	LRR 6
	Repeat	265 – 283	19	LRR 7
	Repeat	284 – 308	25	LRR 8
	Repeat	309 – 332	24	LRR 9
	Repeat	333 – 368	36	LRR 10
	Repeat	369 – 393	25	LRR 11
	Repeat	394 – 426	33	LRR 12
	Repeat	427 – 456	30	LRR 13
	Repeat	457 – 478	22	LRR 14
	Repeat	479 – 500	22	LRR 15
	Repeat	501 – 524	24	LRR 16
	Compositional bias	102 – 517	416	Leu-rich
Experimental info
	Mutagenesis	11	1	L → A: No effects on interactions
	Mutagenesis	22	1	E → A: Abrogates SFC(COI1) complexes formation, loss of response to jasmonate
	Mutagenesis	44	1	W → A: Abrogates SFC(COI1) complexes formation and of interactions with RBCS-1B and RPD3B, loss of response to jasmonate
	Mutagenesis	245	1	L → F in coi1-16; abrogates interactions with RBCS-1B and RPD3B (coi1-16)

Sequences

	Sequence		Length	Mass (Da)
	O04197-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 1DDCF04990144C06	FASTA	592	67,665
	10 20 30 40 50 60 MEDPDIKRCK LSCVATVDDV IEQVMTYITD PKDRDSASLV CRRWFKIDSE TREHVTMALC 70 80 90 100 110 120 YTATPDRLSR RFPNLRSLKL KGKPRAAMFN LIPENWGGYV TPWVTEISNN LRQLKSVHFR 130 140 150 160 170 180 RMIVSDLDLD RLAKARADDL ETLKLDKCSG FTTDGLLSIV THCRKIKTLL MEESSFSEKD 190 200 210 220 230 240 GKWLHELAQH NTSLEVLNFY MTEFAKISPK DLETIARNCR SLVSVKVGDF EILELVGFFK 250 260 270 280 290 300 AAANLEEFCG GSLNEDIGMP EKYMNLVFPR KLCRLGLSYM GPNEMPILFP FAAQIRKLDL 310 320 330 340 350 360 LYALLETEDH CTLIQKCPNL EVLETRNVIG DRGLEVLAQY CKQLKRLRIE RGADEQGMED 370 380 390 400 410 420 EEGLVSQRGL IALAQGCQEL EYMAVYVSDI TNESLESIGT YLKNLCDFRL VLLDREERIT 430 440 450 460 470 480 DLPLDNGVRS LLIGCKKLRR FAFYLRQGGL TDLGLSYIGQ YSPNVRWMLL GYVGESDEGL 490 500 510 520 530 540 MEFSRGCPNL QKLEMRGCCF SERAIAAAVT KLPSLRYLWV QGYRASMTGQ DLMQMARPYW 550 560 570 580 590 NIELIPSRRV PEVNQQGEIR EMEHPAHILA YYSLAGQRTD CPTTVRVLKE PI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"COI1: an Arabidopsis gene required for jasmonate-regulated defense and fertility." Xie D., Feys B.F., James S., Nieto-Rostro M., Turner J.G. Science 280:1091-1094(1998) [PubMed: 9582125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis mutants selected for resistance to the phytotoxin coronatine are male sterile, insensitive to methyl jasmonate, and resistant to a bacterial pathogen." Feys B.J.F., Benedetti C.S., Penfold C.N., Turner J.G. Plant Cell 6:751-759(1994) [PubMed: 12244256] [Abstract] Cited for: FUNCTION. Strain: cv. Columbia.
[5]	"Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis." Reymond P., Weber H., Damond M., Farmer E.E. Plant Cell 12:707-720(2000) [PubMed: 10810145] [Abstract] Cited for: FUNCTION.
[6]	"F-box proteins in Arabidopsis." Xiao W., Jang J.-C. Trends Plant Sci. 5:454-457(2000) [PubMed: 11077244] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"A conditionally fertile coi1 allele indicates cross-talk between plant hormone signalling pathways in Arabidopsis thaliana seeds and young seedlings." Ellis C., Turner J.G. Planta 215:549-556(2002) [PubMed: 12172836] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF LEU-245.
[8]	"The SCF(COI1) ubiquitin-ligase complexes are required for jasmonate response in Arabidopsis." Xu L., Liu F., Lechner E., Genschik P., Crosby W.L., Ma H., Peng W., Huang D., Xie D. Plant Cell 14:1919-1935(2002) [PubMed: 12172031] [Abstract] Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF GLU-22.
[9]	"COI1 links jasmonate signalling and fertility to the SCF ubiquitin-ligase complex in Arabidopsis." Devoto A., Nieto-Rostro M., Xie D., Ellis C., Harmston R., Patrick E., Davis J., Sherratt L., Coleman M., Turner J.G. Plant J. 32:457-466(2002) [PubMed: 12445118] [Abstract] Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF LEU-11; TRP-44 AND LEU-245.
[10]	"Cloning by pathway activation in yeast: identification of an Arabidopsis thaliana F-box protein that can turn on glucose repression." Thelander M., Fredriksson D., Schouten J., Hoge J.H.C., Ronne H. Plant Mol. Biol. 49:69-79(2002) [PubMed: 12008900] [Abstract] Cited for: DOMAIN LEUCINE-RICH REPEATS.
[11]	"The COP9 signalosome interacts physically with SCF COI1 and modulates jasmonate responses." Feng S., Ma L., Wang X., Xie D., Dinesh-Kumar S.P., Wei N., Deng X.W. Plant Cell 15:1083-1094(2003) [PubMed: 12724535] [Abstract] Cited for: FUNCTION, SUBUNIT.
[12]	"Characterization of the early response of Arabidopsis to Alternaria brassicicola infection using expression profiling." van Wees S.C.M., Chang H.-S., Zhu T., Glazebrook J. Plant Physiol. 132:606-617(2003) [PubMed: 12805591] [Abstract] Cited for: FUNCTION.
[13]	"Activation of a COI1-dependent pathway in Arabidopsis by Pseudomonas syringae type III effectors and coronatine." He P., Chintamanani S., Chen Z., Zhu L., Kunkel B.N., Alfano J.R., Tang X., Zhou J.-M. Plant J. 37:589-602(2004) [PubMed: 14756769] [Abstract] Cited for: FUNCTION.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF036340 mRNA. Translation: AAC17498.1. AF002109 Genomic DNA. Translation: AAB95279.1. AY045625 mRNA. Translation: AAK73983.1. AY133556 mRNA. Translation: AAM91386.1.
PIR	T52139.
RefSeq	NP_565919.1.
UniGene	At.71018
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O04197.
Genome annotation databases
GeneID	818581.
GenomeReviews	Gene locus AT2G39940 in contig CT485783_GR.
KEGG	ath:AT2G39940.
NMPDR	fig|3702.1.peg.11117.
Organism-specific databases
GeneFarm	4758.
TAIR	At2g39940.
Gene expression databases
ArrayExpress	O04197.
GermOnline	AT2G39940. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001810. F-box. [Graphical view]
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProDom	O04197. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

COI1_ARATH

Accession

Primary (citable) accession number: O04197

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	July 1, 1997
Last modified:	June 10, 2008
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)