Reviewed, UniProtKB/Swiss-Prot O04300 (UPTG_PEA)

Names and origin

Protein names

Recommended name:
    Alpha-1,4-glucan-protein synthase [UDP-forming]
    EC=2.4.1.-
Alternative name(s):
    UDP-glucose:protein transglucosylase
      Short name=UPTG
    Reversibly glycosylated polypeptide

Gene names

Name:	UPTG
Synonyms:	RGP1

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possible role in the synthesis of cell wall polysaccharides. Ref.1
Catalytic activity	UDP-glucose + protein = UDP + alpha-D-glucosyl-protein. UniProtKB Q9SC19
Enzyme regulation	Inhibited by inhibitor protein (IP) which may be a form of sucrose synthase By similarity.
Subunit structure	Homopentamer or homohexamer By similarity.
Subcellular location	Secreted › cell wall. Cell junction › plasmodesma. Golgi apparatus. Note= Cell wall-associated, with highest concentrations on plasmodesmata. Also located in the Golgi apparatus. Ref.1 Ref.2
Post-translational modification	Reversibly glycosylated by UDP-glucose, UDP-xylose and UDP-galactose, but not UDP-mannose. Ref.1 Ref.2
Sequence similarities	Belongs to the RGP family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Cellulose biosynthesis
Cellular component	Cell junction Cell wall Golgi apparatus Secreted
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: InterPro cell junction Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-KW plant-type cell wall Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-1,4-glucan-protein synthase (UDP-forming) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Alpha-1,4-glucan-protein synthase [UDP-forming]		PRO_0000221194
Amino acid modifications
	Glycosylation	151	1	N-linked (Glc...) By similarity UniProtKB Q9SAQ2

Sequences

	Sequence		Length	Mass (Da)
	O04300-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 006F92870246DF2D	FASTA	364	41,573
	10 20 30 40 50 60 MASLPKPTPL LKDELDIVIP TIRNLDFLEM WRPFFEQYHL IIVQDGDPSK VIKVPEGFDY 70 80 90 100 110 120 ELYNRNDINR ILGPKASCIS FKDSACRCFG YMVSKKKYIY TIDDDCFVAK DPTGHEINAL 130 140 150 160 170 180 EQHIKNLLSP STPFFFNTLY DPYREGTDFV RGYPFSLREG VPTAVSHGLW LNIPDYDAPT 190 200 210 220 230 240 QLVKPHERNT RFVDAVLTIP KGSLFPMCGM NLAFNRELIG PAMYFGLMGD GQPIGRYDDM 250 260 270 280 290 300 WAGWCIKVIC DHLGYGVKTG LPYIWHSKAS NPFVNLKKEY KGIFWQEEII PFFQAATLSK 310 320 330 340 350 360 DCTSVQKCYI ELSKQVKEKL GTIDPYFIKL ADAMVTWVEA WDEINNNKSE ETTSTKASEV AATK

References

[1]

"A reversibly glycosylated polypeptide (RGP1) possibly involved in plant cell wall synthesis: purification, gene cloning, and trans-Golgi localization." Dhugga K.S., Tiwari S.C., Ray P.M. Proc. Natl. Acad. Sci. U.S.A. 94:7679-7684(1997) [PubMed: 9207152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-37 AND 159-173, SUBCELLULAR LOCATION, GLYCOSYLATION. Strain: cv. Early Alaska. Tissue: Stem.

[2]

"Plant polypeptides reversibly glycosylated by UDP-glucose. Possible components of Golgi beta-glucan synthase in pea cells." Dhugga K.S., Ulvskov P., Gallagher S.R., Ray P.M. J. Biol. Chem. 266:21977-21984(1991) [PubMed: 1834664] [Abstract] Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U31565 mRNA. Translation: AAB88408.1.
PIR	T06507.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR004901. UPTG_synth. [Graphical view]
Pfam	PF03214. RGP. 1 hit. [Graphical view]
PIRSF	PIRSF016429. UPTG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UPTG_PEA

Accession

Primary (citable) accession number: O04300

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 9, 2003
Last sequence update:	July 1, 1997
Last modified:	November 25, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)