Reviewed, UniProtKB/Swiss-Prot O07921 (CHIS_BACSU)

Names and origin

Protein names

Recommended name:
    Chitosanase
    EC=3.2.1.132

Gene names

Name:	csn
Ordered Locus Names:	BSU26890

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Aids in the defense against invading fungal pathogens by degrading their cell wall chitosan By similarity.
Catalytic activity	Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan.
Subcellular location	SecretedBy similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 46 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	chitosanase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Chain	36 – 277	242	Chitosanase		PRO_0000012207
Sites
	Active site	54	1	Proton donor By similarity
	Active site	70	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	O07921-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 8C17C156AF27F781	FASTA	277	31,497
	10 20 30 40 50 60 MKISMQKADF WKKAAISLLV FTMFFTLMMS ETVFAAGLNK DQKRRAEQLT SIFENGTTEI 70 80 90 100 110 120 QYGYVERLDD GRGYTCGRAG FTTATGDALE VVEVYTKAVP NNKLKKYLPE LRRLAKEESD 130 140 150 160 170 180 DTSNLKGFAS AWKSLANDKE FRAAQDKVND HLYYQPAMKR SDNAGLKTAL ARAVMYDTVI 190 200 210 220 230 240 QHGDGDDPDS FYALIKRTNK KAGGSPKDGI DEKKWLNKFL DVRYDDLMNP ANHDTRDEWR 250 260 270 ESVARVDVLR SIAKENNYNL NGPIHVRSNE YGNFVIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 23911 bp region of the Bacillus subtilis genome comprising genes located upstream and downstream of the lev operon." Parro V., San Roman M., Galindo I., Purnelle B., Bolotin A., Sorokin A., Mellado R.P. Microbiology 143:1321-1326(1997) [PubMed: 9141695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"Sequence of the Bacillus subtilis genome region in the vicinity of the lev operon reveals two new extracytoplasmic function RNA polymerase sigma factors SigV and SigZ." Sorokin A., Bolotin A., Purnelle B., Hilbert H., Lauber J., Duesterhoeft A., Ehrlich S.D. Microbiology 143:2939-2943(1997) [PubMed: 9308178] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X92868 Genomic DNA. Translation: CAA63455.1. U93875 Genomic DNA. Translation: AAB80882.1. Z99117 Genomic DNA. Translation: CAB14630.1.
PIR	C69608.
RefSeq	NP_390566.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHK based on UniProtKB P33665.
ModBase	Search...
Genome annotation databases
GeneID	936802.
GenomeReviews	Gene locus BSU26890 in contig AL009126_GR.
KEGG	bsu:BSU26890.
NMPDR	fig|224308.1.peg.2691.
Organism-specific databases
SubtiList	BG11923. csn. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	O07921.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2684-MON.
Family and domain databases
InterPro	IPR000400. Glyco_hydro_46. [Graphical view]
Pfam	PF01374. Glyco_hydro_46. 1 hit. [Graphical view]
PIRSF	PIRSF036551. Chitosanase. 1 hit.
PROSITE	PS60000. CHITOSANASE_46_80. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIS_BACSU

Accession

Primary (citable) accession number: O07921

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 1, 1997
Last modified:	November 25, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)