Reviewed, UniProtKB/Swiss-Prot P72059 (EMBC_MYCTU)

Names and origin

Protein names

Recommended name:
    Probable arabinosyltransferase C
    EC=2.4.2.-

Gene names

Name:	embC
Ordered Locus Names:	Rv3793, MT3900
ORF Names:	MTCY13D12.27

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	1094 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.
Subcellular location	Cell membrane; Multi-pass membrane proteinProbable.
Miscellaneous	This is one of the targets of the anti-tuberculosis drug ethambutol [(S,S')-2,2'-(ethylenediimino)di-1-butanol; EMB]. EMB is a first-line drug used to treat tuberculosis. EMB inhibits the transfer of arabinogalactan into the cell wall.
Sequence similarities	Belongs to the emb family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring pentosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1094	1094	Probable arabinosyltransferase C		PRO_0000220573
Regions
	Transmembrane	28 – 50	23	Potential
	Transmembrane	232 – 251	20	Potential
	Transmembrane	264 – 286	23	Potential
	Transmembrane	341 – 360	20	Potential
	Transmembrane	373 – 392	20	Potential
	Transmembrane	431 – 453	23	Potential
	Transmembrane	466 – 488	23	Potential
	Transmembrane	530 – 552	23	Potential
	Transmembrane	565 – 582	18	Potential
	Transmembrane	586 – 608	23	Potential
	Transmembrane	620 – 642	23	Potential
	Transmembrane	657 – 679	23	Potential
	Transmembrane	700 – 722	23	Potential
Natural variations
	Natural variant	394	1	N → D Resistance to EMB. Ref.4
	Natural variant	738	1	R → Q Resistance to EMB. Ref.4
Experimental info
	Sequence conflict	981	1	V → L in AAK48266. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P72059-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: AF8190BE0A1EA05E	FASTA	1,094	117,490
	10 20 30 40 50 60 MATEAAPPRI AVRLPSTSVR DAGANYRIAR YVAVVAGLLG AVLAIATPLL PVNQTTAQLN 70 80 90 100 110 120 WPQNGTFASV EAPLIGYVAT DLNITVPCQA AAGLAGSQNT GKTVLLSTVP KQAPKAVDRG 130 140 150 160 170 180 LLLQRANDDL VLVVRNVPLV TAPLSQVLGP TCQRLTFTAH ADRVAAEFVG LVQGPNAEHP 190 200 210 220 230 240 GAPLRGERSG YDFRPQIVGV FTDLAGPAPP GLSFSASVDT RYSSSPTPLK MAAMILGVAL 250 260 270 280 290 300 TGAALVALHI LDTADGMRHR RFLPARWWST GGLDTLVIAV LVWWHFVGAN TSDDGYILTM 310 320 330 340 350 360 ARVSEHAGYM ANYYRWFGTP EAPFGWYYDL LALWAHVSTA SIWMRLPTLA MALTCWWVIS 370 380 390 400 410 420 REVIPRLGHA VKTSRAAAWT AAGMFLAVWL PLDNGLRPEP IIALGILLTW CSVERAVATS 430 440 450 460 470 480 RLLPVAIACI IGALTLFSGP TGIASIGALL VAIGPLRTIL HRRSRRFGVL PLVAPILAAA 490 500 510 520 530 540 TVTAIPIFRD QTFAGEIQAN LLKRAVGPSL KWFDEHIRYE RLFMASPDGS IARRFAVLAL 550 560 570 580 590 600 VLALAVSVAM SLRKGRIPGT AAGPSRRIIG ITIISFLAMM FTPTKWTHHF GVFAGLAGSL 610 620 630 640 650 660 GALAAVAVTG AAMRSRRNRT VFAAVVVFVL ALSFASVNGW WYVSNFGVPW SNSFPKWRWS 670 680 690 700 710 720 LTTALLELTV LVLLLAAWFH FVANGDGRRT ARPTRFRARL AGIVQSPLAI ATWLLVLFEV 730 740 750 760 770 780 VSLTQAMISQ YPAWSVGRSN LQALAGKTCG LAEDVLVELD PNAGMLAPVT APLADALGAG 790 800 810 820 830 840 LSEAFTPNGI PADVTADPVM ERPGDRSFLN DDGLITGSEP GTEGGTTAAP GINGSRARLP 850 860 870 880 890 900 YNLDPARTPV LGSWRAGVQV PAMLRSGWYR LPTNEQRDRA PLLVVTAAGR FDSREVRLQW 910 920 930 940 950 960 ATDEQAAAGH HGGSMEFADV GAAPAWRNLR APLSAIPSTA TQVRLVADDQ DLAPQHWIAL 970 980 990 1000 1010 1020 TPPRIPRVRT LQNVVGAADP VFLDWLVGLA FPCQRPFGHQ YGVDETPKWR ILPDRFGAEA 1030 1040 1050 1060 1070 1080 NSPVMDHNGG GPLGITELLM RATTVASYLK DDWFRDWGAL QRLTPYYPDA QPADLNLGTV 1090 TRSGLWSPAP LRRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol." Telenti A., Philipp W.J., Sreevatsan S., Bernasconi C., Stockbauer K.E., Wieles B., Musser J.M., Jacobs W.R. Jr. Nat. Med. 3:567-570(1997) [PubMed: 9142129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[4]	"Molecular genetic analysis of nucleotide polymorphisms associated with ethambutol resistance in human isolates of Mycobacterium tuberculosis." Ramaswamy S.V., Amin A.G., Goeksel S., Stager C.E., Dou S.-J., El Sahly H., Moghazeh S.L., Kreiswirth B.N., Musser J.M. Antimicrob. Agents Chemother. 44:326-336(2000) [PubMed: 10639358] [Abstract] Cited for: VARIANTS EMB RESISTANT ASP-394 AND GLN-738.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842584 Genomic DNA. Translation: CAB02472.1. U68480 Genomic DNA. Translation: AAC45279.1. AE000516 Genomic DNA. Translation: AAK48266.1.
PIR	E70697.
RefSeq	NP_218310.1. NP_338452.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	886112. 922602.
GenomeReviews	Gene locus MT3900 in contig AE000516_GR. Gene locus Rv3793 in contig AL123456_GR.
KEGG	mtc:MT3900. mtu:Rv3793.
TIGR	MT3900.
Organism-specific databases
TubercuList	Rv3793.
Phylogenomic databases
HOGENOM	P72059.
Family and domain databases
InterPro	IPR007680. Arab_transf. [Graphical view]
Pfam	PF04602. Arabinose_trans. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00330. Ethambutol.

Entry information

Entry name

EMBC_MYCTU

Accession

Primary (citable) accession number: P72059
Secondary accession number(s): O08116

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	February 1, 1997
Last modified:	November 25, 2008
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)