Reviewed, UniProtKB/Swiss-Prot O09043 (NAPSA_MOUSE)

Names and origin

Protein names

Recommended name:
    Napsin-A
    EC=3.4.23.-
Alternative name(s):
    Kidney-derived aspartic protease-like protein
    KDAP-1
      Short name=KAP

Gene names

Name:	Napsa
Synonyms:	Kdap, Nap

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in processing of pneumocyte surfactant precursors By similarity.
Tissue specificity	Expressed at the highest levels in the kidney, at a moderate level in the lung, and at low levels in the spleen and adipose tissue.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Glycoprotein Zymogen
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – ?		Activation peptide Potential
	Chain	? – 419		Napsin-A
Sites
	Active site	91	1	By similarity
	Active site	278	1	By similarity
Amino acid modifications
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	128	1	N-linked (GlcNAc...)
	Glycosylation	149	1	N-linked (GlcNAc...) Potential
	Glycosylation	331	1	N-linked (GlcNAc...) Potential
	Disulfide bond	104 ↔ 111		By similarity
	Disulfide bond	269 ↔ 273		By similarity
	Disulfide bond	312 ↔ 349		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O09043-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 715ED4532791143E	FASTA	419	45,544
	10 20 30 40 50 60 MSPLLLLLLC LLLGNLEPEE AKLIRVPLQR IHLGHRILNP LNGWEQLAEL SRTSTSGGNP 70 80 90 100 110 120 SFVPLSKFMN TQYFGTIGLG TPPQNFTVVF DTGSSNLWVP STRCHFFSLA CWFHHRFNPK 130 140 150 160 170 180 ASSSFRPNGT KFAIQYGTGR LSGILSQDNL TIGGIHDAFV TFGEALWEPS LIFALAHFDG 190 200 210 220 230 240 ILGLGFPTLA VGGVQPPLDA MVEQGLLEKP VFSFYLNRDS EGSDGGELVL GGSDPAHYVP 250 260 270 280 290 300 PLTFIPVTIP AYWQVHMESV KVGTGLSLCA QGCSAILDTG TSLITGPSEE IRALNKAIGG 310 320 330 340 350 360 YPFLNGQYFI QCSKTPTLPP VSFHLGGVWF NLTGQDYVIK ILQSDVGLCL LGFQALDIPK 370 380 390 400 410 PAGPLWILGD VFLGPYVAVF DRGDKNVGPR VGLARAQSRS TDRAERRTTQ AQFFKRRPG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel mouse aspartic protease-like protein that is expressed abundantly in the kidney." Mori K., Ogawa Y., Tamura N., Ebihara K., Aoki T., Muro S., Ozaki S., Tanaka I., Tashiro K., Nakao K. FEBS Lett. 401:218-222(1997) [PubMed: 9013890] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Kidney.
[2]	"Molecular organization, expression and chromosomal localization of the mouse pronapsin gene." Tatnell P.J., Cook M., Peters C., Kay J. Eur. J. Biochem. 267:6921-6930(2000) [PubMed: 11082205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
[4]	"Proteome-wide characterization of N-glycosylation events by diagonal chromatography." Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K. J. Proteome Res. 5:2438-2447(2006) [PubMed: 16944957] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-128, MASS SPECTROMETRY. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D88899 mRNA. Translation: BAA19004.1. AJ250718, AJ250719, AJ250720 Genomic DNA. Translation: CAB82907.1. BC014813 mRNA. Translation: AAH14813.1.
RefSeq	NP_032463.1.
UniGene	Mm.383181
3D structure databases
HSSP	HSSP built from PDB template 2REN based on UniProtKB P00797.
ModBase	Search...
Protein family/group databases
MEROPS	A01.046.
Genome annotation databases
Ensembl	ENSMUSG00000002204. Mus musculus. [Contig view]
GeneID	16541.
KEGG	mmu:16541.
Organism-specific databases
MGI	MGI:109365. Napsa.
Phylogenomic databases
HOGENOM	O09043.
HOVERGEN	O09043.
Gene expression databases
ArrayExpress	O09043.
CleanEx	MM_NAPSA.
GermOnline	ENSMUSG00000002204. Mus musculus.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. IPR001461. Peptidase_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProDom	O09043. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

NAPSA_MOUSE

Accession

Primary (citable) accession number: O09043

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	July 1, 1997
Last modified:	July 22, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)