Reviewed, UniProtKB/Swiss-Prot O13716 (AGN1_SCHPO)

Names and origin

Protein names

Recommended name:
    Glucan endo-1,3-alpha-glucosidase agn1
    EC=3.2.1.59
Alternative name(s):
    Endo-1,3-alpha-glucanase agn1

Gene names

Name:	agn1
ORF Names:	SPAC14C4.09

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has a role in cell separation where it is required for the degradation of the cell wall material surrounding the septum (the septum edging) which must be hydrolyzed before full separation of the daughter cells can occur. Hydrolyzes 1,3-alpha-glucan predominantly into pentasaccharides. Ref.1
Catalytic activity	Endohydrolysis of (1->3)-alpha-D-glucosidic linkages in isolichenin, pseudonigeran and nigeran.
Subunit structure	Monomer. Ref.1
Subcellular location	Secreted. Secreted › cell wall. Note= Associates with the cell wall. Ref.1
Post-translational modification	Not glycosylated.
Sequence similarities	Belongs to the glycosyl hydrolase 71 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell septum edging catabolic process Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oligosaccharide catabolic process Inferred by curator. Source: GeneDB_SPombe
Cellular component	cell septum edging Ref.1 Inferred from direct assay. Source: GeneDB_SPombe cell surface Ref.1 Inferred from direct assay. Source: GeneDB_SPombe extracellular region Ref.1 Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucan endo-1,3-alpha-glucosidase activity Ref.1 Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Probable
	Chain	21 – 424	404	Glucan endo-1,3-alpha-glucosidase agn1		PRO_0000012111

Sequences

	Sequence		Length	Mass (Da)
	O13716-1 [UniParc]. Last modified August 16, 2004. Version 2. Checksum: 2D680FDB8C56AEBF	FASTA	424	46,974
	10 20 30 40 50 60 MKLVLFLVLL FSALINLTNA DKMVVAHFIV GNTYPYTVSN WEEDIQDAIA VGIDGFALNM 70 80 90 100 110 120 GSDAWQVERI EDAYDAAASV SSDFKLFISF DMSIISADAD FIEGVVRRFA DKPNQLYYDG 130 140 150 160 170 180 KVFVSTFAGE TDTFGYSDVS TGWDSAVKEP LASAGYPIYF VPSWTSLGQG ALEESVADGF 190 200 210 220 230 240 LSWNAWPTTD ADMNDNDDIG YQNLANSLGK LYVAPVSPWF YTHLSYKNWA YKSDWLIIDR 250 260 270 280 290 300 WNEMLSVQPD MIEVLTWNDY GESHYIGNIQ GALPAGSEGY VDGFDHTAWR YLMSPYISAY 310 320 330 340 350 360 KLGLSEPYIN FESLFYWYRP TPKSATATAD SLSYPSGGDY MEDEIFVLVY LLQSAEVTVT 370 380 390 400 410 420 CGSTTQTFSG VPGVNQFTIP METNASPSFT VARQGGTLAS GTGPEIVDSL SIYNFNAYTG VLYF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Role of the alpha-glucanase Agn1p in fission-yeast cell separation." Dekker N., Speijer D., Grun C.H., Van Den Berg M., De Haan A., Hochstenbach F. Mol. Biol. Cell 15:3903-3914(2004) [PubMed: 15194814] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION. Strain: ATCC 38366 / 972.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY626901 mRNA. Translation: AAT84064.1. CU329670 Genomic DNA. Translation: CAG47121.1.
PIR	T37694.
RefSeq	NP_001018296.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3361560.
KEGG	spo:SPAC14C4.09.
NMPDR	fig|4896.1.peg.4884.
Organism-specific databases
GeneDB_Spombe	SPAC14C4.09.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-002979-MON.
Gene expression databases
ArrayExpress	O13716.
Family and domain databases
InterPro	IPR005197. Glyco_hydro_71. [Graphical view]
Pfam	PF03659. Glyco_hydro_71. 1 hit. [Graphical view]
ProDom	PD139156. Glyco_hydro_71. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

AGN1_SCHPO

Accession

Primary (citable) accession number: O13716
Secondary accession number(s): Q6F6J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)