Reviewed, UniProtKB/Swiss-Prot O13728 (AIN1_SCHPO)

Names and origin

Protein names

Recommended name:
    Alpha-actinin-like protein 1

Gene names

Name:	ain1
ORF Names:	SPAC15A10.08

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Binds to actin and is involved in actin-ring formation and organization. Plays a role in cytokinesis and is involved in septation. Ref.2
Subcellular location	Note= Localizes to the medial ring in an F-actin-dependent manner. Ref.2
Sequence similarities	Belongs to the alpha-actinin family. Contains 1 actin-binding domain. Contains 2 CH (calponin-homology) domains. Contains 3 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Ligand	Actin-binding Calcium
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	actin filament bundle formation Traceable author statement. Source: GeneDB_SPombe cytokinesis, contractile ring formation Ref.2 Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	contractile ring Ref.2 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	actin binding Ref.2 Inferred from electronic annotation. Source: InterPro calcium ion binding Inferred from electronic annotation. Source: InterPro protein binding, bridging Traceable author statement. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	Alpha-actinin-like protein 1		PRO_0000073448
Regions
	Domain	8 – 114	107	CH 1
	Domain	86 – 110	25	Actin-binding
	Domain	123 – 227	105	CH 2
	Domain	388 – 419	32	EF-hand 1
	Domain	487 – 549	63	EF-hand 2
	Domain	550 – 618	69	EF-hand 3

Sequences

	Sequence		Length	Mass (Da)
	O13728-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 50D7544D17942C0C	FASTA	621	72,443
	10 20 30 40 50 60 MQANQWQSVQ NRTFTKWFNT KLSSRDLPSV FDLRKDLSDG ILLIQLLEII GDENLGRYNR 70 80 90 100 110 120 NPRMRVHRLE NVNKALEYIK SKGMPLTNIG PADIVDGNLK LILGLIWTLI LRFTIADINE 130 140 150 160 170 180 EGLTAKEGLL LWCQRKTANY HPEVDVQDFT RSWTNGLAFC ALIHQHRPDL LDYNKLDKKN 190 200 210 220 230 240 HRANMQLAFD IAQKSIGIPR LIEVEDVCDV DRPDERSIMT YVAEYFHAFS TLDKVETAAR 250 260 270 280 290 300 RVERFTEVLM STHDMKIDYE SRMKRLLGSI ARMQEYWHTV QFENNYTDVK SHSNNFAKFK 310 320 330 340 350 360 ATEKREWVKE KIDLESLLGT IQTNLKTYQL RKYEPPAGLK IVDLERQWKD FLSEEANQSK 370 380 390 400 410 420 LINTHMREIK ESMRIAFADR ANSFSKMLST ISNEITNLQG DWRDQLDHVE FLQEHLGPLE 430 440 450 460 470 480 VELASVKVLY DNCFQAGIEE NDYTMFSYED LEHEFGITAN IIANKIKYLE NELLEREKRT 490 500 510 520 530 540 LSKQELDGIT KVFRHFEKKK SNMLNEVEFY AALASLGLVY DTEEGTALFH RAANSEEGVT 550 560 570 580 590 600 YERFTEIVME ELEDRDSARQ VLYAFCDVAD GKSYVTSDDL LRSQVRPNIV KFLECNMNKH 610 620 SEGLDYLTWI KQLLAEDKEI V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Roles of a fimbrin and an alpha-actinin-like protein in fission yeast cell polarization and cytokinesis." Wu J.-Q., Baehler J., Pringle J.R. Mol. Biol. Cell 12:1061-1077(2001) [PubMed: 11294907] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAB10105.1.
PIR	T37708.
RefSeq	NP_594295.1.
3D structure databases
HSSP	HSSP built from PDB template 1BKR based on UniProtKB Q01082.
ModBase	Search...
Genome annotation databases
GeneID	2542805.
KEGG	spo:SPAC15A10.08.
NMPDR	fig|4896.1.peg.4265.
Organism-specific databases
GeneDB_Spombe	SPAC15A10.08.
Gene expression databases
ArrayExpress	O13728.
Family and domain databases
InterPro	IPR001589. Actinin_actin-bd_CS. IPR016146. Calponin-homology. IPR001715. Calponin_act_bd. IPR014837. EF-hand_Ca_insen. IPR011992. EF-Hand_type. IPR002048. EF_hand_Ca_bd. [Graphical view]
Gene3D	G3DSA:1.10.418.10. Calponin-homology. 2 hits. G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF00307. CH. 2 hits. PF08726. efhand_Ca_insen. 1 hit. [Graphical view]
SMART	SM00033. CH. 2 hits. [Graphical view]
PROSITE	PS00019. ACTININ_1. False negative. PS00020. ACTININ_2. 1 hit. PS50021. CH. 2 hits. PS00018. EF_HAND_1. False negative. PS50222. EF_HAND_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AIN1_SCHPO

Accession

Primary (citable) accession number: O13728

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	January 1, 1998
Last modified:	November 25, 2008
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)