Reviewed, UniProtKB/Swiss-Prot O13953 (EAF3_SCHPO)

Names and origin

Protein names

Recommended name:
    Chromatin modification-related protein eaf3
Alternative name(s):
    Altered polarity protein 13
    ESA1-associated factor 3

Gene names

Name:	alp13
Synonyms:	eaf3
ORF Names:	SPAC23H4.12

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair By similarity. Also involved in deacetylation of histones, chromatin assembly and chromosome segregation. May act as a transcriptional oscillator, directing histone deacetylases to specific chromosomal domains.
Subunit structure	Component of the NuA4 histone acetyltransferase complex By similarity. Heterotetramer of alp13, clr6, prw1 and pst2.
Subcellular location	Nucleus. Ref.3
Sequence similarities	Belongs to the MRG family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Chromatin regulator
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW chromatin assembly or disassembly Inferred from electronic annotation. Source: InterPro chromatin remodeling Inferred by curator. Source: GeneDB_SPombe chromosome condensation Ref.3 Inferred from mutant phenotype. Source: GeneDB_SPombe establishment or maintenance of cell polarity Inferred from mutant phenotype. Source: GeneDB_SPombe histone deacetylation Ref.3 Inferred from mutant phenotype. Source: GeneDB_SPombe regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Clr6 histone deacetylase complex II' Ref.3 Inferred from direct assay. Source: GeneDB_SPombe nuclear chromatin Inferred by curator. Source: GeneDB_SPombe
Molecular function	chromatin binding Inferred from electronic annotation. Source: InterPro protein binding Ref.3 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pst2	O13919	1	EBI-904711,EBI-904686

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Chromatin modification-related protein eaf3		PRO_0000088780

Sequences

	Sequence		Length	Mass (Da)
	O13953-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 827B85CE12CAE8CC	FASTA	337	39,138
	10 20 30 40 50 60 MAVSYKVNER VLCFHGPLLY EAKIVDTEMK GDVTTYLIHY KGWKNSWDEW VEQDRILQWT 70 80 90 100 110 120 EENLKTQKEL KNAAISTRQK PTSKKSASST SKHDSTGVKT SGKRSRESST VTVDGDSHEL 130 140 150 160 170 180 PSRIKTQKSE SPIPQQVKRD GTTDAKNEET TKPENNEKDD FEEEPPLPKH KISVPDVLKL 190 200 210 220 230 240 WLVDDWENIT KNQQLIAIPR NPTVRAAIAA FRESKISHLN NEIDVDVFEQ AMAGLVIYFN 250 260 270 280 290 300 KCLGNMLLYR FERQQYLEIR QQYPDTEMCD LYGVEHLIRL FVSLPELIDR TNMDSQSIEC 310 320 330 LLNYIEEFLK YLVLHKDEYF IKEYQNAPPN YRSLVGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Chromo domain protein required for cell polarity." Radcliffe P.A., Toda T. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Alp13, an MRG family protein, is a component of fission yeast Clr6 histone deacetylase required for genomic integrity." Nakayama J., Xiao G., Noma K., Malikzay A., Bjerling P., Ekwall K., Kobayashi R., Grewal S.I.S. EMBO J. 22:2776-2787(2003) [PubMed: 12773392] [Abstract] Cited for: PROTEIN SEQUENCE OF 105-123; 129-138; 147-169 AND 311-332, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB015294 Genomic DNA. Translation: BAA28826.1. CU329670 Genomic DNA. Translation: CAB11666.1.
PIR	T43372.
RefSeq	NP_593394.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O13953. 5 interactions.
Genome annotation databases
GeneID	2541858.
KEGG	spo:SPAC23H4.12.
NMPDR	fig|4896.1.peg.3364.
Organism-specific databases
GeneDB_Spombe	SPAC23H4.12.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001081-MON.
Gene expression databases
ArrayExpress	O13953.
Family and domain databases
InterPro	IPR000953. Chromodomain. IPR017398. Hist_Nua4_cplx_EAF3/MRG15_su. IPR008676. MRG. [Graphical view]
PANTHER	PTHR10880. MRG. 1 hit.
Pfam	PF05712. MRG. 1 hit. [Graphical view]
PIRSF	PIRSF038133. HAT_Nua4_EAF3/MRG15. 1 hit.
SMART	SM00298. CHROMO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EAF3_SCHPO

Accession

Primary (citable) accession number: O13953

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	December 16, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)