Reviewed, UniProtKB/Swiss-Prot O14228 (PUR4_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	ade3
Synonyms:	min11
ORF Names:	SPAC6F12.10c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H(2)O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/5.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1323	1323	Probable phosphoribosylformylglycinamidine synthase		PRO_0000100404
Regions
	Domain	1062 – 1306	245	Glutamine amidotransferase type-1
	Nucleotide binding	312 – 323	12	ATP Potential
Sites
	Active site	1156	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O14228-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7D31A6BEFAE2997F	FASTA	1,323	144,867
	10 20 30 40 50 60 MLVYYGGSAL SVQSKKKILE LTVSGVSDLN AVYFYLIYTK SNSSLNCESL RPILSDLQES 70 80 90 100 110 120 EFKPDGTTMV YVFPRPGTIS PWSSKATNIA NVCGYKDVIR IERGIAYSVV FKDDISEEML 130 140 150 160 170 180 KSALNHLYDR MTEALRFKLP EEDEVFDKHE PAPLVRIELN CGQGGDKQAA TERLNHANKK 190 200 210 220 230 240 FGLALAPDEI DYLVECYTSE PSLKSREPTD VELFMFGQVN SEHCRHKIFN ADWTIDGEKK 250 260 270 280 290 300 DYSLFKMIRN THLKNPQYTI SAYSDNAAVF EGNSGTLFAP VNGIWSMKDE PVEFLGKVET 310 320 330 340 350 360 HNHPTAVSPF PGAATGSGGE IRDEGAVGQG SLSKAGLAGY SVSDLNIPGY KQPWELDVGK 370 380 390 400 410 420 PYHIATSLDI MLEAPIGSSA FNNEFGRPCI NGYFRTFCME VPRGDGTLEI RGYHKPIMAA 430 440 450 460 470 480 GGIGRIRKQH AFKKSIAPGS PIIVLGGPAL LVGLGGGAAS SMNAGEGSEE LDFASVQRGN 490 500 510 520 530 540 PEMQRRAQMV IDACTTMDEN IIQSIHDVGA GGVSNALPEL VHDAGLGARF ELRDIPCIEP 550 560 570 580 590 600 SMSPMQIWCC ESQERYVLSV KSEDLDTFKS ICERERCPYG VVGYSTVEQR LILTDRLYNT 610 620 630 640 650 660 TPIDLPMEVL FGKPPKMSRV AETQTIPLSK FDSSLKSYLA PSSDPILDAV ERVLRMPAVA 670 680 690 700 710 720 SKSFLITIGD RSVTGLIARD QMVGPWQVPV ADVGVTVTSY GKGINTGEAL AMGEKPISAL 730 740 750 760 770 780 VSAAASARMA VAECIMNLVA ASIPALDRIR LSANWMAAPS HPGEGAKLYE AVQAIGLELC 790 800 810 820 830 840 PSLGISIPVG KDSMSMSMKW NEDGREKSVT APLSLIITGF SPVDDLYSIW TPQLRKVEDI 850 860 870 880 890 900 GSTSLIFIDL ANGKQRLGGS ILAQSYKQLG DEVPDLDNLD TFKNFINVIT QLHKTNYIQA 910 920 930 940 950 960 YHDKSDGGLF VTLSEMAFAG HVGIECELDS LSSDNIAALF NEELGAVIQV CDRDIAKVLE 970 980 990 1000 1010 1020 LFAANGLSTC VHRIGKVLSG QAQTISFSRS GKIIFKSTRS KLHGIWHETS YKMQEIRDNP 1030 1040 1050 1060 1070 1080 ECARQEMENI ADNNDPGLGY HLTFDPNVSV TADLALTSRP KVAILREQGV NGYLEMAYAF 1090 1100 1110 1120 1130 1140 YASGFTAVDV HMTDILSGRV HLDDFVGIAA CGGFSYGDVL GSGNGWATSI LLHEDARNEF 1150 1160 1170 1180 1190 1200 YRFFNERKDT FGLGICNGCQ LFSRLKSLIP GAKSWPMFTF NESAQYEGRA VMLKIDETSG 1210 1220 1230 1240 1250 1260 SKSIFTESMA GSSLPVVVAH GEGRAVFDSE SDYEQFKKEG LDLIYYVNNY NERTSRYPFN 1270 1280 1290 1300 1310 1320 PNGSRDAIAG VRSPCGRFLA MMPHPERVVL KVANSYYPHS KASEWGVHGP WIRLFQSARK WVG

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAB11094.1.
PIR	T11661.
RefSeq	NP_593296.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2542871.
KEGG	spo:SPAC6F12.10c.
NMPDR	fig|4896.1.peg.3266.
Organism-specific databases
GeneDB_Spombe	SPAC6F12.10c.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000995-MON.
Gene expression databases
ArrayExpress	O14228.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_SCHPO

Accession

Primary (citable) accession number: O14228

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	January 1, 1998
Last modified:	November 25, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)