Reviewed, UniProtKB/Swiss-Prot O14730 (RIOK3_HUMAN)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase RIO3
      Short name=RIO kinase 3
    EC=2.7.11.1
Alternative name(s):
    sudD homolog

Gene names

Name:	RIOK3
Synonyms:	SUDD

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Widely expressed. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	chromosome segregation Ref.1 Traceable author statement. Source: ProtInc protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARL1	P40616	1	EBI-1047061,EBI-1052746
CALM1	P62158	1	EBI-1047061,EBI-397435
FBL	P22087	1	EBI-1047061,EBI-358318
MAGEA6	P43360	1	EBI-1047061,EBI-1045155
PSMB1	P20618	1	EBI-1047061,EBI-372273
PSMC4	P43686	1	EBI-1047061,EBI-743997
RUVBL2	Q9Y230	1	EBI-1047061,EBI-352939
SSR4	P51571	1	EBI-1047061,EBI-1054539

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O14730-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O14730-2)

The sequence of this isoform differs from the canonical sequence as follows:
     449-451: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 519	519	Serine/threonine-protein kinase RIO3		PRO_0000213529
Regions
	Domain	251 – 519	269	Protein kinase
	Nucleotide binding	257 – 265	9	ATP By similarity
Sites
	Active site	406	1	Proton acceptor By similarity
	Binding site	290	1	ATP By similarity
Amino acid modifications
	Modified residue	122	1	Phosphotyrosine Ref.4
	Modified residue	127	1	Phosphoserine Ref.4
	Modified residue	128	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	449 – 451	3	Missing in isoform 2.		VSP_012164
	Natural variant	336	1	L → V: dbSNP rs35401850.		VAR_042355
	Natural variant	441	1	R → Q: dbSNP rs33969048.		VAR_042356
	Natural variant	447	1	S → L Ref.5		VAR_042357
Experimental info
	Sequence conflict	325	1	I → H in AAC26080. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 20, 2007. Version 2. Checksum: 3C3392BAC4ECB912	FASTA	519	59,093
	10 20 30 40 50 60 MDLVGVASPE PGTAAAWGPS KCPWAIPQNT ISCSLADVMS EQLAKELQLE EEAAVFPEVA 70 80 90 100 110 120 VAEGPFITGE NIDTSSDLML AQMLQMEYDR EYDAQLRREE KKFNGDSKVS ISFENYRKVH 130 140 150 160 170 180 PYEDSDSSED EVDWQDTRDD PYRPAKPVPT PKKGFIGKGK DITTKHDEVV CGRKNTARME 190 200 210 220 230 240 NFAPEFQVGD GIGMDLKLSN HVFNALKQHA YSEERRSARL HEKKEHSTAE KAVDPKTRLL 250 260 270 280 290 300 MYKMVNSGML ETITGCISTG KESVVFHAYG GSMEDEKEDS KVIPTECAIK VFKTTLNEFK 310 320 330 340 350 360 NRDKYIKDDF RFKDRFSKLN PRKIIRMWAE KEMHNLARMQ RAGIPCPTVV LLKKHILVMS 370 380 390 400 410 420 FIGHDQVPAP KLKEVKLNSE EMKEAYYQTL HLMRQLYHEC TLVHADLSEY NMLWHAGKVW 430 440 450 460 470 480 LIDVSQSVEP THPHGLEFLF RDCRNVSQFF QKGGVKEALS ERELFNAVSG LNITADNEAD 490 500 510 FLAEIEALEK MNEDHVQKNG RKAASFLKDD GDPPLLYDE
	Isoform 2. Checksum: 2CA9BC88DD00CFE5		516	58,671
	10 20 30 40 50 60 MDLVGVASPE PGTAAAWGPS KCPWAIPQNT ISCSLADVMS EQLAKELQLE EEAAVFPEVA 70 80 90 100 110 120 VAEGPFITGE NIDTSSDLML AQMLQMEYDR EYDAQLRREE KKFNGDSKVS ISFENYRKVH 130 140 150 160 170 180 PYEDSDSSED EVDWQDTRDD PYRPAKPVPT PKKGFIGKGK DITTKHDEVV CGRKNTARME 190 200 210 220 230 240 NFAPEFQVGD GIGMDLKLSN HVFNALKQHA YSEERRSARL HEKKEHSTAE KAVDPKTRLL 250 260 270 280 290 300 MYKMVNSGML ETITGCISTG KESVVFHAYG GSMEDEKEDS KVIPTECAIK VFKTTLNEFK 310 320 330 340 350 360 NRDKYIKDDF RFKDRFSKLN PRKIIRMWAE KEMHNLARMQ RAGIPCPTVV LLKKHILVMS 370 380 390 400 410 420 FIGHDQVPAP KLKEVKLNSE EMKEAYYQTL HLMRQLYHEC TLVHADLSEY NMLWHAGKVW 430 440 450 460 470 480 LIDVSQSVEP THPHGLEFLF RDCRNVSQKG GVKEALSERE LFNAVSGLNI TADNEADFLA 490 500 510 EIEALEKMNE DHVQKNGRKA ASFLKDDGDP PLLYDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of the Aspergillus nidulans sudD gene and its human homologue." Anaya P., Evans S.C., Dai C., Lozano G., May G.S. Gene 211:323-329(1998) [PubMed: 9602165] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"DNA sequence and analysis of human chromosome 18." Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S. Nature 437:551-555(2005) [PubMed: 16177791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-122; SER-127 AND SER-128, MASS SPECTROMETRY.
[5]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] VAL-336; GLN-441 AND LEU-447.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF013591 mRNA. Translation: AAC26080.1. AC026634 Genomic DNA. No translation available. BC039729 mRNA. Translation: AAH39729.1.
RefSeq	NP_003822.2.
UniGene	Hs.445511
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O14730. 8 interactions.
PTM databases
PhosphoSite	O14730.
Proteomic databases
PRIDE	O14730.
Genome annotation databases
Ensembl	ENSG00000101782. Homo sapiens. [Contig view]
GeneID	8780.
KEGG	hsa:8780.
Organism-specific databases
GeneCards	GC18P019287.
H-InvDB	HIX0014365.
HGNC	HGNC:11451. RIOK3.
HPA	HPA001620.
MIM	603579. gene.
PharmGKB	PA36248.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O14730.
HOVERGEN	O14730.
Gene expression databases
ArrayExpress	O14730.
CleanEx	HS_RIOK3.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR000687. RIO_kinase. IPR017406. Ser/Thr_prot_kinase_Rio3. [Graphical view]
Pfam	PF01163. RIO1. 1 hit. [Graphical view]
PIRSF	PIRSF038146. Ser/Thr_PK_RIO3. 1 hit.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00090. RIO. 1 hit. [Graphical view]
PROSITE	PS50011. PROTEIN_KINASE_DOM. False negative. PS01245. RIO1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	O14730.
NextBio	32920.
SOURCE	Search...

Entry information

Entry name

RIOK3_HUMAN

Accession

Primary (citable) accession number: O14730
Secondary accession number(s): Q8IXN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	February 20, 2007
Last modified:	December 16, 2008
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)