Reviewed, UniProtKB/Swiss-Prot O15085 (ARHGB_HUMAN)

Names and origin

Protein names

Recommended name:
    Rho guanine nucleotide exchange factor 11
Alternative name(s):
    PDZ-RhoGEF

Gene names

Name:	ARHGEF11
Synonyms:	KIAA0380

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1522 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.
Subunit structure	Interacts with GNA12 and GNA13 through the RGS domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts with SLC1A6 By similarity.
Subcellular location	Cytoplasm. Membrane. Note= Translocated to the membrane upon stimulation. Ref.7
Tissue specificity	Ubiquitously expressed. Ref.4
Domain	The poly-Pro region is essential for plasma membrane localization upon stimulation.
Sequence similarities	Contains 1 DH (DBL-homology) domain. Contains 1 PDZ (DHR) domain. Contains 1 PH domain. Contains 1 RGSL (RGS-like) domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Polymorphism
Domain	Coiled coil
Molecular function	GTPase activation Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Ref.4 Traceable author statement. Source: UniProtKB actin cytoskeleton organization Ref.4 Non-traceable author statement. Source: UniProtKB cell motion Ref.4 Non-traceable author statement. Source: UniProtKB cytokinesis Ref.4 Non-traceable author statement. Source: UniProtKB establishment of cell polarity Ref.4 Non-traceable author statement. Source: UniProtKB positive regulation of transcription, DNA-dependent Ref.4 Inferred from direct assay. Source: UniProtKB regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro regulation of cell growth Ref.4 Non-traceable author statement. Source: UniProtKB striated muscle contraction Ref.4 Non-traceable author statement. Source: UniProtKB
Cellular component	cytoplasm Inferred from direct assay. Source: MGI membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	G-protein-coupled receptor binding Inferred from direct assay. Source: MGI GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW Rho guanyl-nucleotide exchange factor activity Ref.4 Traceable author statement. Source: UniProtKB signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABCA1	O95477	1	EBI-311099,EBI-784112
ABL1	P00519	1	EBI-311099,EBI-375543
CRK	P46108	1	EBI-311099,EBI-886
FYN	P06241	1	EBI-311099,EBI-515315
GRB2	P62993	1	EBI-311099,EBI-401755
NCK1	P16333	1	EBI-311099,EBI-389883
PIK3R1	P27986	1	EBI-311099,EBI-79464
PLCG1	P19174	1	EBI-311099,EBI-79387

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1522

1522

Rho guanine nucleotide exchange factor 11

PRO_0000080928

Regions

Domain

47 – 126

80

PDZ

Domain

306 – 486

181

RGSL

Domain

734 – 923

190

DH

Domain

965 – 1079

115

PH

Coiled coil

444 – 470

27

Potential

Compositional bias

1094 – 1099

6

Poly-Pro

Amino acid modifications

Modified residue

245

1

Phosphoserine Ref.10

Modified residue

251

1

Phosphoserine Ref.10

Modified residue

254

1

Phosphothreonine Ref.10

Modified residue

255

1

Phosphoserine Ref.10

Modified residue

663

1

Phosphoserine Ref.8 Ref.9

Modified residue

668

1

Phosphothreonine Ref.10 Ref.8 Ref.9

Modified residue

672

1

Phosphothreonine Ref.10 Ref.9

Modified residue

1155

1

Phosphoserine Ref.10 Ref.8

Modified residue

1295

1

Phosphoserine Ref.10

Modified residue

1300

1

Phosphoserine Ref.10

Modified residue

1457

1

Phosphoserine Ref.10

Modified residue

1458

1

Phosphoserine Ref.10

Modified residue

1462

1

Phosphothreonine Ref.10

Modified residue

1475

1

Phosphothreonine Ref.10

Modified residue

1480

1

Phosphoserine Ref.10

Modified residue

1489

1

Phosphoserine Ref.10

Modified residue

1491

1

Phosphoserine Ref.10

Natural variations

Natural variant

1427

1

H → R: dbSNP rs945508.

VAR_024285

Secondary structure

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1522

Helix Strand Turn

Details...

Beta strand

45 – 51

7

Beta strand

53 – 56

4

Beta strand

59 – 62

4

Beta strand

64 – 66

3

Beta strand

68 – 72

5

Beta strand

74 – 76

3

Turn

77 – 82

6

Beta strand

88 – 92

5

Beta strand

98 – 100

3

Helix

102 – 109

8

Beta strand

111 – 120

10

Helix

307 – 312

6

Helix

314 – 319

6

Helix

321 – 334

14

Helix

338 – 349

12

Helix

356 – 368

13

Helix

381 – 392

12

Helix

398 – 424

27

Helix

428 – 431

4

Turn

432 – 434

3

Helix

435 – 438

4

Helix

443 – 462

20

Helix

466 – 482

17

Turn

716 – 718

3

Beta strand

719 – 724

6

Helix

725 – 727

3

Helix

730 – 759

30

Helix

761 – 766

6

Helix

772 – 778

7

Beta strand

779 – 781

3

Helix

782 – 801

20

Helix

810 – 817

8

Helix

819 – 833

15

Helix

836 – 849

14

Helix

851 – 861

11

Helix

864 – 866

3

Helix

871 – 874

4

Helix

877 – 894

18

Helix

901 – 939

39

Turn

943 – 945

3

Turn

951 – 953

3

Helix

954 – 958

5

Helix

961 – 963

3

Beta strand

966 – 974

9

Beta strand

977 – 979

3

Beta strand

983 – 998

16

Beta strand

1024 – 1027

4

Helix

1028 – 1030

3

Beta strand

1031 – 1035

5

Beta strand

1040 – 1047

8

Beta strand

1050 – 1052

3

Beta strand

1056 – 1060

5

Helix

1064 – 1080

17

Sequences

	Sequence		Length	Mass