Reviewed, UniProtKB/Swiss-Prot O15145 (ARPC3_HUMAN)

Names and origin

Protein names

Recommended name:
    Actin-related protein 2/3 complex subunit 3
Alternative name(s):
    Arp2/3 complex 21 kDa subunit
    p21-ARC

Gene names

Name:	ARPC3
Synonyms:	ARC21

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
Subunit structure	Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.
Subcellular location	Cytoplasm › cytoskeleton. Cell projection.
Sequence similarities	Belongs to the ARPC3 family.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm Cytoskeleton
Ligand	Actin-binding
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell motility Ref.2 Traceable author statement. Source: ProtInc
Cellular component	Arp2/3 protein complex Ref.2 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural constituent of cytoskeleton Ref.2 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARP3beta	Q9P1U1	1	EBI-351829,EBI-1047175

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 178	177	Actin-related protein 2/3 complex subunit 3
Amino acid modifications
	Modified residue	47	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	134	1	Q → P in AAB61466. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O15145-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 7149F598B48F0EAC	FASTA	178	20,547
	10 20 30 40 50 60 MPAYHSSLMD PDTKLIGNMA LLPIRSQFKG PAPRETKDTD IVDEAIYYFK ANVFFKNYEI 70 80 90 100 110 120 KNEADRTLIY ITLYISECLK KLQKCNSKSQ GEKEMYTLGI TNFPIPGEPG FPLNAIYAKP 130 140 150 160 170 ANKQEDEVMR AYLQQLRQET GLRLCEKVFD PQNDKPSKWW TCFVKRQFMN KSLSGPGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mammalian actin-related protein 2/3 complex localizes to regions of lamellipodial protrusion and is composed of evolutionarily conserved proteins." Machesky L.M., Reeves E., Wientjes F., Mattheyse F.J., Grogan A., Totty N.F., Burlingame A.L., Hsuan J.J., Segal A.W. Biochem. J. 328:105-112(1997) [PubMed: 9359840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]	"The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly." Welch M.D., Depace A.H., Verma S., Iwamatsu A., Mitchison T.J. J. Cell Biol. 138:375-384(1997) [PubMed: 9230079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION IN THE ARP2/2 COMPLEX, SUBCELLULAR LOCATION.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Skin.
[5]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-25. Tissue: Platelet.
[6]	"Reconstitution of human Arp2/3 complex reveals critical roles of individual subunits in complex structure and activity." Gournier H., Goley E.D., Niederstrasser H., Trinh T., Welch M.D. Mol. Cell 8:1041-1052(2001) [PubMed: 11741539] [Abstract] Cited for: RECONSTITUTION OF THE ARP2/3 COMPLEX.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF004561 mRNA. Translation: AAB61466.1. AF006086 mRNA. Translation: AAB64191.1. CR407667 mRNA. Translation: CAG28595.1. BC067747 mRNA. Translation: AAH67747.1. BC078162 mRNA. Translation: AAH78162.1.
RefSeq	NP_005710.1.
UniGene	Hs.524741
3D structure databases
SMR	O15145. Positions 1-173, 2-174.
ModBase	Search...
Protein-protein interaction databases
IntAct	O15145.
PTM databases
PhosphoSite	O15145.
2-D gel databases
OGP	O15145.
Genome annotation databases
Ensembl	ENSG00000111229. Homo sapiens. [Contig view]
GeneID	10094.
KEGG	hsa:10094.
Organism-specific databases
H-InvDB	HIX0036676.
HGNC	HGNC:706. ARPC3.
HPA	HPA006550.
MIM	604225. gene.
PharmGKB	PA25000.
GenAtlas	Search...
GeneCards	Search...
GeneLynx	Search...
Phylogenomic databases
HOGENOM	O15145.
HOVERGEN	O15145.
Gene expression databases
CleanEx	HS_ARPC3.
GermOnline	ENSG00000111229. Homo sapiens.
Family and domain databases
InterPro	IPR007204. ARP2/3_P21-Arc. [Graphical view]
Gene3D	G3DSA:1.10.1760.10. ARP2/3_P21-Arc. 1 hit.
PANTHER	PTHR12391. P21-Arc. 1 hit.
Pfam	PF04062. P21-Arc. 1 hit. [Graphical view]
PIRSF	PIRSF016315. ARP2/3_P21-Arc. 1 hit.
ProDom	O15145. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	O15145.
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

ARPC3_HUMAN

Accession

Primary (citable) accession number: O15145
Secondary accession number(s): O00554

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	July 22, 2008
This is version 61 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)