Arachidonate 15-lipoxygenase type II

Names and origin

Protein names

Recommended name:
    Arachidonate 15-lipoxygenase type II
      Short name=15-LOX-2
    EC=1.13.11.33
Alternative name(s):
    15-lipoxygenase 2

Gene names

Name:

ALOX15B

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	676 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized.
Catalytic activity	Arachidonate + O(2) = (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate.
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Lipid metabolism; leukotriene D4 biosynthesis.
Subcellular location	CytoplasmBy similarity.
Tissue specificity	Expressed in hair, prostate, lung and cornea.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

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Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	induction of apoptosis Ref.3 Traceable author statement. Source: UniProtKB leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of cell cycle Ref.3 Inferred from direct assay. Source: UniProtKB negative regulation of cell migration Ref.3 Traceable author statement. Source: UniProtKB negative regulation of cell proliferation Ref.3 Inferred from direct assay. Source: UniProtKB negative regulation of growth Ref.3 Inferred from direct assay. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW prostate gland development Ref.3 Non-traceable author statement. Source: UniProtKB regulation of epithelial cell differentiation Ref.3 Non-traceable author statement. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arachidonate 15-lipoxygenase activity Ref.3 Inferred from direct assay. Source: UniProtKB iron ion binding Inferred from electronic annotation. Source: InterPro lipoxygenase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

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Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: O15296-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: O15296-2) Also known as: 15-LOX2sv-b; The sequence of this isoform differs from the canonical sequence as follows: 401-429: Missing. 483-527: Missing.

Isoform C (identifier: O15296-3) Also known as: 15-LOX2sv-c; The sequence of this isoform differs from the canonical sequence as follows: 561-617: FDSCAWMPNL...WLLSKEPGDQ → VRKGQRPRWQ...PKAWQHARAS 618-676: Missing.

Isoform D (identifier: O15296-4) Also known as: 15-LOX2sv-a; The sequence of this isoform differs from the canonical sequence as follows: 401-429: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 676

676

Arachidonate 15-lipoxygenase type II

PRO_0000220700

Regions

Domain

2 – 124

123

PLAT

Domain

125 – 676

552

Lipoxygenase

Sites

Metal binding

373

1

Iron; catalytic By similarity

Metal binding

378

1

Iron; catalytic By similarity

Metal binding

553

1

Iron; catalytic By similarity

Metal binding

676

1

Iron; via carboxylate; catalytic By similarity

Natural variations

Alternative sequence

401 – 429

29

Missing in isoform B and isoform D.

VSP_003142

Alternative sequence

483 – 527

45

Missing in isoform B.

VSP_003143

Alternative sequence

561 – 617

57

FDSCA…EPGDQ → VRKGQRPRWQAGGDPAPQPH SALSAFSLTPVLGCPTCHPA CSCHHPPPKAWQHARAS in isoform C.

VSP_003144

Alternative sequence

618 – 676

59

Missing in isoform C.

VSP_003145

Natural variant

647

1

I → V: dbSNP rs7225107.

VAR_035040

Natural variant

656

1

R → Q: dbSNP rs4792147.

VAR_024524

Natural variant

676

1

I → V: dbSNP rs7225107.

VAR_024525

Experimental info

Sequence conflict

271

1

V → L in AAB61706. Ref.1

Sequence conflict

271

1

V → L in CAC34521. Ref.2

Sequence conflict

338

1

G → C in AAD37786. Ref.5

Sequence conflict

486

1

R → H in AAL76274, AAL76275 and AAL76277. Ref.3

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Sequences

Sequence		Length	Mass (Da)
Isoform A [UniParc]. Last modified September 11, 2007. Version 2. Checksum: 4F641DF2FBB902C6 Show »	FASTA	676	75,885
10 20 30 40 50 60 MAEFRVRVST GEAFGAGTWD KVSVSIVGTR GESPPLPLDN LGKEFTAGAE EDFQVTLPED 70 80 90 100 110 120 VGRVLLLRVH KAPPVLPLLG PLAPDAWFCR WFQLTPPRGG HLLFPCYQWL EGAGTLVLQE 130 140 150 160 170 180 GTAKVSWADH HPVLQQQRQE ELQARQEMYQ WKAYNPGWPH CLDEKTVEDL ELNIKYSTAK 190 200 210 220 230 240 NANFYLQAGS AFAEMKIKGL LDRKGLWRSL NEMKRIFNFR RTPAAEHAFE HWQEDAFFAS 250 260 270 280 290 300 QFLNGLNPVL IRRCHYLPKN FPVTDAMVAS VLGPGTSLQA ELEKGSLFLV DHGILSGIQT 310 320 330 340 350 360 NVINGKPQFS AAPMTLLYQS PGCGPLLPLA IQLSQTPGPN SPIFLPTDDK WDWLLAKTWV 370 380 390 400 410 420 RNAEFSFHEA LTHLLHSHLL PEVFTLATLR QLPHCHPLFK LLIPHTRYTL HINTLARELL 430 440 450 460 470 480 IVPGQVVDRS TGIGIEGFSE LIQRNMKQLN YSLLCLPEDI RTRGVEDIPG YYYRDDGMQI 490 500 510 520 530 540 WGAVERFVSE IIGIYYPSDE SVQDDRELQA WVREIFSKGF LNQESSGIPS SLETREALVQ 550 560 570 580 590 600 YVTMVIFTCS AKHAAVSAGQ FDSCAWMPNL PPSMQLPPPT SKGLATCEGF IATLPPVNAT 610 620 630 640 650 660 CDVILALWLL SKEPGDQRPL GTYPDEHFTE EAPRRSIATF QSRLAQISRG IQERNRGLVL 670 PYTYLDPPLI ENSVSI « Hide
Isoform B (15-LOX2sv-b). Checksum: 8804BB440B3B05C2 Show »		602	67,373
10 20 30 40 50 60 MAEFRVRVST GEAFGAGTWD KVSVSIVGTR GESPPLPLDN LGKEFTAGAE EDFQVTLPED 70 80 90 100 110 120 VGRVLLLRVH KAPPVLPLLG PLAPDAWFCR WFQLTPPRGG HLLFPCYQWL EGAGTLVLQE 130 140 150 160 170 180 GTAKVSWADH HPVLQQQRQE ELQARQEMYQ WKAYNPGWPH CLDEKTVEDL ELNIKYSTAK 190 200 210 220 230 240 NANFYLQAGS AFAEMKIKGL LDRKGLWRSL NEMKRIFNFR RTPAAEHAFE HWQEDAFFAS 250 260 270 280 290 300 QFLNGLNPVL IRRCHYLPKN FPVTDAMVAS VLGPGTSLQA ELEKGSLFLV DHGILSGIQT 310 320 330 340 350 360 NVINGKPQFS AAPMTLLYQS PGCGPLLPLA IQLSQTPGPN SPIFLPTDDK WDWLLAKTWV 370 380 390 400 410 420 RNAEFSFHEA LTHLLHSHLL PEVFTLATLR QLPHCHPLFK STGIGIEGFS ELIQRNMKQL 430 440 450 460 470 480 NYSLLCLPED IRTRGVEDIP GYYYRDDGMQ IWGIPSSLET REALVQYVTM VIFTCSAKHA 490 500 510 520 530 540 AVSAGQFDSC AWMPNLPPSM QLPPPTSKGL ATCEGFIATL PPVNATCDVI LALWLLSKEP 550 560 570 580 590 600 GDQRPLGTYP DEHFTEEAPR RSIATFQSRL AQISRGIQER NRGLVLPYTY LDPPLIENSV SI « Hide
Isoform C (15-LOX2sv-c). Checksum: 3F690488B2B766AC Show »		617	69,136
10 20 30 40 50 60 MAEFRVRVST GEAFGAGTWD KVSVSIVGTR GESPPLPLDN LGKEFTAGAE EDFQVTLPED 70 80 90 100 110 120 VGRVLLLRVH KAPPVLPLLG PLAPDAWFCR WFQLTPPRGG HLLFPCYQWL EGAGTLVLQE 130 140 150 160 170 180 GTAKVSWADH HPVLQQQRQE ELQARQEMYQ WKAYNPGWPH CLDEKTVEDL ELNIKYSTAK 190 200 210 220 230 240 NANFYLQAGS AFAEMKIKGL LDRKGLWRSL NEMKRIFNFR RTPAAEHAFE HWQEDAFFAS 250 260 270 280 290 300 QFLNGLNPVL IRRCHYLPKN FPVTDAMVAS VLGPGTSLQA ELEKGSLFLV DHGILSGIQT 310 320 330 340 350 360 NVINGKPQFS AAPMTLLYQS PGCGPLLPLA IQLSQTPGPN SPIFLPTDDK WDWLLAKTWV 370 380 390 400 410 420 RNAEFSFHEA LTHLLHSHLL PEVFTLATLR QLPHCHPLFK LLIPHTRYTL HINTLARELL 430 440 450 460 470 480 IVPGQVVDRS TGIGIEGFSE LIQRNMKQLN YSLLCLPEDI RTRGVEDIPG YYYRDDGMQI 490 500 510 520 530 540 WGAVERFVSE IIGIYYPSDE SVQDDRELQA WVREIFSKGF LNQESSGIPS SLETREALVQ 550 560 570 580 590 600 YVTMVIFTCS AKHAAVSAGQ VRKGQRPRWQ AGGDPAPQPH SALSAFSLTP VLGCPTCHPA 610 CSCHHPPPKA WQHARAS « Hide
Isoform D (15-LOX2sv-a). Checksum: EE51EEA920715C4B Show »		647	72,551
10 20 30 40 50 60 MAEFRVRVST GEAFGAGTWD KVSVSIVGTR GESPPLPLDN LGKEFTAGAE EDFQVTLPED 70 80 90 100 110 120 VGRVLLLRVH KAPPVLPLLG PLAPDAWFCR WFQLTPPRGG HLLFPCYQWL EGAGTLVLQE 130 140 150 160 170 180 GTAKVSWADH HPVLQQQRQE ELQARQEMYQ WKAYNPGWPH CLDEKTVEDL ELNIKYSTAK 190 200 210 220 230 240 NANFYLQAGS AFAEMKIKGL LDRKGLWRSL NEMKRIFNFR RTPAAEHAFE HWQEDAFFAS 250 260 270 280 290 300 QFLNGLNPVL IRRCHYLPKN FPVTDAMVAS VLGPGTSLQA ELEKGSLFLV DHGILSGIQT 310 320 330 340 350 360 NVINGKPQFS AAPMTLLYQS PGCGPLLPLA IQLSQTPGPN SPIFLPTDDK WDWLLAKTWV 370 380 390 400 410 420 RNAEFSFHEA LTHLLHSHLL PEVFTLATLR QLPHCHPLFK STGIGIEGFS ELIQRNMKQL 430 440 450 460 470 480 NYSLLCLPED IRTRGVEDIP GYYYRDDGMQ IWGAVERFVS EIIGIYYPSD ESVQDDRELQ 490 500 510 520 530 540 AWVREIFSKG FLNQESSGIP SSLETREALV QYVTMVIFTC SAKHAAVSAG QFDSCAWMPN 550 560 570 580 590 600 LPPSMQLPPP TSKGLATCEG FIATLPPVNA TCDVILALWL LSKEPGDQRP LGTYPDEHFT 610 620 630 640 EEAPRRSIAT FQSRLAQISR GIQERNRGLV LPYTYLDPPL IENSVSI « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Discovery of a second 15S-lipoxygenase in humans." Brash A.R., Boeglin W.E., Chang M.S. Proc. Natl. Acad. Sci. U.S.A. 94:6148-6152(1997) [PubMed: 9177185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Tissue: Skin.
[2]	"A gene cluster encoding human epidermis-type lipoxygenases at chromosome 17p13.1: cloning, physical mapping, and expression." Krieg P., Marks F., Fuerstenberger G. Genomics 73:323-330(2001) [PubMed: 11350124] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells." Tang S., Bhatia B., Maldonado C.J., Yang P., Newman R.A., Liu J., Chandra D., Traag J., Klein R.D., Fischer S.M., Chopra D., Shen J., Zhau H.E., Chung L.W.K., Tang D.G. J. Biol. Chem. 277:16189-16201(2002) [PubMed: 11839751] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C AND D).
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Tissue: Skin.
[5]	"Differential characteristics of human 15-lipoxygenase isozymes and a novel splice variant of 15S-lipoxygenase." Kilty I., Logan A., Vickers P.J. Eur. J. Biochem. 266:83-93(1999) [PubMed: 10542053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 337-484 (ISOFORM B).
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	U78294 mRNA. Translation: AAB61706.1. AJ305028 , AJ305029, AJ305030, AJ305031 Genomic DNA. Translation: CAC34521.1. AF468051 mRNA. Translation: AAL76274.1. AF468052 mRNA. Translation: AAL76275.1. AF468053 mRNA. Translation: AAL76276.1. AF468054 mRNA. Translation: AAL76277.1. BC035217 mRNA. Translation: AAH35217.1. BC063647 mRNA. Translation: AAH63647.1. AF149095 Genomic DNA. Translation: AAD37786.1. Sequence problems.
RefSeq	NP_001034219.1. NP_001034220.1. NP_001132.2.
UniGene	Hs.111256
3D structure databases
HSSP	HSSP built from PDB template 1LOX based on UniProtKB P12530.
ModBase	Search...
PTM databases
PhosphoSite	O15296.
Genome annotation databases
Ensembl	ENSG00000179593. Homo sapiens. [Contig view]
GeneID	247.
KEGG	hsa:247.
Organism-specific databases
GeneCards	GC17P007883.
H-InvDB	HIX0027139.
HGNC	HGNC:434. ALOX15B.
MIM	603697. gene.
PharmGKB	PA24725.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	O15296.
Gene expression databases
ArrayExpress	O15296.
CleanEx	HS_ALOX15B.
GermOnline	ENSG00000179593. Homo sapiens.
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001885. LipOase_mml. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00467. MAMLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	985.
SOURCE	Search...

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Entry information

Entry name

LX15B_HUMAN

Accession

Primary (citable) accession number: O15296
Secondary accession number(s): Q8IYQ2

Q9UKM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	September 11, 2007
Last modified:	December 16, 2008
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents