Reviewed, UniProtKB/Swiss-Prot O19000 (MT1_CANFA)

Names and origin

Protein names

Recommended name:
    Metallothionein-1
      Short name=MT-1
Alternative name(s):
    Metallothionein-I
      Short name=MT-I

Gene names

Name:

MT1

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Metal-binding Metal-thiolate cluster Zinc
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 61	61	Metallothionein-1		PRO_0000197198
Regions
	Region	1 – 29	29	Beta
	Region	30 – 61	32	Alpha
Sites
	Metal binding	5	1	Cluster B
	Metal binding	7	1	Cluster B
	Metal binding	13	1	Cluster B
	Metal binding	15	1	Cluster B
	Metal binding	19	1	Cluster B
	Metal binding	21	1	Cluster B
	Metal binding	24	1	Cluster B
	Metal binding	26	1	Cluster B
	Metal binding	29	1	Cluster B
	Metal binding	33	1	Cluster A
	Metal binding	34	1	Cluster A
	Metal binding	36	1	Cluster A
	Metal binding	37	1	Cluster A
	Metal binding	41	1	Cluster A
	Metal binding	44	1	Cluster A
	Metal binding	48	1	Cluster A
	Metal binding	50	1	Cluster A
	Metal binding	57	1	Cluster A
	Metal binding	59	1	Cluster A
	Metal binding	60	1	Cluster A

Sequences

	Sequence		Length	Mass (Da)
	O19000-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 9C127654E4954062	FASTA	61	6,031
	10 20 30 40 50 60 MDPDCSCSTG GSCTCAGSCK CKECKCTSCK KSCCSCCPVG CAKCAQGCIC KGASDKCSCC A

References

[1]	"Canine hepatic lysosomal copper protein: identification as metallothionein." Lerch K., Johnson G.F., Grushoff P.S., Sternlieb I. Arch. Biochem. Biophys. 243:108-114(1985) [PubMed: 4062298] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Liver.
[2]	"Molecular cloning of a canine metallothionein cDNA." Kobayashi K., Shimada A., Yamano Y., Umemura T. J. Vet. Med. Sci. 59:819-823(1997) [PubMed: 9342709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Beagle. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D84397 mRNA. Translation: BAA22941.1.
RefSeq	NP_001003173.1.
UniGene	Cfa.3664
3D structure databases
HSSP	HSSP built from PDB template 1MHU based on UniProtKB P02795.
SMR	O19000. Positions 1-61.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCAFG00000009113. Canis familiaris. [Contig view]
GeneID	403800.
KEGG	cfa:403800.
Phylogenomic databases
HOVERGEN	O19000.
Family and domain databases
InterPro	IPR002400. GF_cysknot. IPR003019. Metallothionein_sfam_euk. IPR000006. Metallothionein_vert. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00438. GFCYSKNOT. PR00860. MTVERTEBRATE.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MT1_CANFA

Accession

Primary (citable) accession number: O19000

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	November 4, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)