Reviewed, UniProtKB/Swiss-Prot O22775 (GT2_ARATH)

Names and origin

Protein names

Recommended name:
    Putative glycosyltransferase 2
      Short name=AtGT2
    EC=2.4.-.-

Gene names

Name:	GT2
Synonyms:	GTL5
Ordered Locus Names:	At4g02500
ORF Names:	T14P8.23, T10P11.20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Subcellular location	Golgi apparatus membrane; Single-pass type II membrane proteinPotential.
Sequence similarities	Belongs to the glycosyltransferase 34 family.
Sequence caution	The sequence AAC78266.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB80743.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	root hair elongation Inferred from genetic interaction. Source: TAIR xyloglucan biosynthetic process Inferred from genetic interaction. Source: TAIR
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	UDP-xylosyltransferase activity Inferred from direct assay. Source: TAIR transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro xyloglucan 6-xylosyltransferase activity Inferred from genetic interaction. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Putative glycosyltransferase 2		PRO_0000215170
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 40	20	Signal-anchor for type II membrane protein Potential
	Topological domain	41 – 461	421	Lumenal Potential
Amino acid modifications
	Glycosylation	432	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O22775-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A5B98B5BCF110F98	FASTA	461	53,095
	10 20 30 40 50 60 MIERCLGAYR CRRIQRALRQ LKVTILCLLL TVVVLRSTIG AGKFGTPEQD LDEIRQHFHA 70 80 90 100 110 120 RKRGEPHRVL EEIQTGGDSS SGDGGGNSGG SNNYETFDIN KIFVDEGEEE KPDPNKPYTL 130 140 150 160 170 180 GPKISDWDEQ RSDWLAKNPS FPNFIGPNKP RVLLVTGSAP KPCENPVGDH YLLKSIKNKI 190 200 210 220 230 240 DYCRLHGIEI FYNMALLDAE MAGFWAKLPL IRKLLLSHPE IEFLWWMDSD AMFTDMAFEL 250 260 270 280 290 300 PWERYKDYNL VMHGWNEMVY DQKNWIGLNT GSFLLRNNQW ALDLLDTWAP MGPKGKIREE 310 320 330 340 350 360 AGKVLTRELK DRPVFEADDQ SAMVYLLATQ RDAWGNKVYL ESGYYLHGYW GILVDRYEEM 370 380 390 400 410 420 IENYHPGLGD HRWPLVTHFV GCKPCGKFGD YPVERCLKQM DRAFNFGDNQ ILQIYGFTHK 430 440 450 460 SLASRKVKRV RNETSNPLEM KDELGLLHPA FKAVKVQTNQ V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel Arabidopsis Golgi glycosyltransferase." Mogelsvang S., Dupree P. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"An Arabidopsis gene encoding an alpha-xylosyltransferase involved in xyloglucan biosynthesis." Faik A., Price N.J., Raikhel N.V., Keegstra K. Proc. Natl. Acad. Sci. U.S.A. 99:7797-7802(2002) [PubMed: 12032363] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ245571 mRNA. Translation: CAC01674.1. AF069298 Genomic DNA. Translation: AAC19271.1. AC002330 Genomic DNA. Translation: AAC78266.1. Sequence problems. AL161494 Genomic DNA. Translation: CAB80743.1. Sequence problems. AY057598 mRNA. Translation: AAL14393.1. AY140029 mRNA. Translation: AAM98170.1. BT006601 mRNA. Translation: AAP31945.1.
PIR	H85031. T01300.
RefSeq	NP_567241.1.
UniGene	At.21214
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	O22775.
Genome annotation databases
GeneID	827940.
GenomeReviews	Gene locus AT4G02500 in contig CT486007_GR.
KEGG	ath:AT4G02500.
NMPDR	fig|3702.1.peg.18064.
Organism-specific databases
TAIR	At4g02500.
Gene expression databases
ArrayExpress	O22775.
GermOnline	AT4G02500. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008630. Glyco_trans_34. [Graphical view]
Pfam	PF05637. Glyco_transf_34. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GT2_ARATH

Accession

Primary (citable) accession number: O22775
Secondary accession number(s): Q9SYY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	January 1, 1998
Last modified:	December 16, 2008
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)