Reviewed, UniProtKB/Swiss-Prot O23878 (13S1_FAGES)

Names and origin

Protein names

Recommended name:
    13S globulin seed storage protein 1
Alternative name(s):
    Legumin-like protein 1
Cleaved into the following 2 chains:
    1- Recommended name:
            13S globulin seed storage protein 1 acidic chain
    2- Recommended name:
            13S globulin seed storage protein 1 basic chain

Gene names

Name:

FA02

Organism

Fagopyrum esculentum (Common buckwheat)

Taxonomic identifier

3617 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Polygonaceae › Fagopyrum

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Seed storage protein.
Subunit structure	Hexamer; each subunit is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond By similarity.
Tissue specificity	Expressed only in immature seeds. Ref.1
Developmental stage	Expressed between 7 and 28 days after pollination. Ref.1
Miscellaneous	The sequence of the probable beta chain is highly homologous to the N-terminal sequence of BW24KD, a major buckwheat allergen.
Sequence similarities	Belongs to the 11S seed storage protein (globulins) family.

Ontologies

Keywords
Domain	Signal
Molecular function	Seed storage protein Storage protein
Gene Ontology (GO)
Molecular function	nutrient reservoir activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 377	357	13S globulin seed storage protein 1 acidic chain By similarity		PRO_0000032003
	Chain	378 – 565	188	13S globulin seed storage protein 1 basic chain By similarity		PRO_0000032004
Amino acid modifications
	Disulfide bond	44 ↔ 77		By similarity
	Disulfide bond	120 ↔ 384		Interchain (between acidic and basic chains) Potential

Sequences

	Sequence		Length	Mass (Da)
	O23878-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 2DD7FCC64E3CD4F0	FASTA	565	64,518
	10 20 30 40 50 60 MSTKLILSFS LCLMVLSCSA QLLPWRKGQR SRPHRGHQQF HHQCDVQRLT ASEPSRRVRS 70 80 90 100 110 120 EAGVTEIWDN DTPEFRCAGF VAVRVVIQPG GLLLPSYSNA PYITFVEQGR GVQGVVVPGC 130 140 150 160 170 180 PETFQSESEF EYPQSQRDQR SRQSESEESS RGDQRTRQSE SEEFSRGDQR TRQSESEEFS 190 200 210 220 230 240 RGDQRTRQSE SEEFSRGDQR TRQSESEEFS RGDQHQKIFR IRDGDVIPSP AGVVQWTHND 250 260 270 280 290 300 GDNDLISITL YDANSFQNQL DGNVRNFFLA GQSKQSREDR RSQRQTREEG SDRQSRESDD 310 320 330 340 350 360 DEALLEANIL TGFQDEILQE IFRNVDQETI SKLRGDNDQR GFIVQARDLK LRVPEEYEEE 370 380 390 400 410 420 LQRERGDRKR GGSGRSNGLE QAFCNLKFKQ NVNRPSRADV FNPRAGRINT VNSNNLPILE 430 440 450 460 470 480 FIQLSAQHVV LYKNAILGPR WNLNAHSALY VTRGEGRVQV VGDEGRSVFD DNVQRGQILV 490 500 510 520 530 540 VPQGFAVVLK AGREGLEWVE LKNDDNAITS PIAGKTSVLR AIPVEVLANS YDISTKEAFR 550 560 LKNGRQEVEV FLPFQSRDEK ERERF

References

[1]

"Expression, cloning, and immunological analysis of buckwheat (Fagopyrum esculentum Moench) seed storage proteins." Fujino K., Funatsuki H., Inada M., Shimono Y., Kikuta Y. J. Agric. Food Chem. 49:1825-1829(2001) [PubMed: 11308332] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. Kitayuki. Tissue: Immature seed.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D87980 mRNA. Translation: BAA21758.1.
PIR	T10696.
3D structure databases
HSSP	HSSP built from PDB template 1FXZ based on UniProtKB P04776.
ModBase	Search...
Family and domain databases
InterPro	IPR006045. Cupin_1. IPR014710. RmlC-like_jellyroll. IPR006044. Seedstore11s_pln. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF00190. Cupin_1. 2 hits. [Graphical view]
PRINTS	PR00439. 11SGLOBULIN.
ProtoNet	Search...

Entry information

Entry name

13S1_FAGES

Accession

Primary (citable) accession number: O23878

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	January 1, 1998
Last modified:	November 25, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)