Reviewed, UniProtKB/Swiss-Prot O23880 (13S2_FAGES)

Names and origin

Protein names

Recommended name:
    13S globulin seed storage protein 2
Alternative name(s):
    Legumin-like protein 2
Cleaved into the following 2 chains:
    1- Recommended name:
            13S globulin seed storage protein 2 acidic chain
    2- Recommended name:
            13S globulin seed storage protein 2 basic chain

Gene names

Name:

FA18

Organism

Fagopyrum esculentum (Common buckwheat)

Taxonomic identifier

3617 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Polygonaceae › Fagopyrum

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Seed storage protein.
Subunit structure	Hexamer; each subunit is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond By similarity.
Miscellaneous	The sequence of the probable beta chain is highly homologous to the N-terminal sequence of BW24KD, a major buckwheat allergen.
Sequence similarities	Belongs to the 11S seed storage protein (globulins) family.

Ontologies

Keywords
Domain	Signal
Molecular function	Seed storage protein Storage protein
Gene Ontology (GO)
Molecular function	nutrient reservoir activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 313	293	13S globulin seed storage protein 2 acidic chain By similarity		PRO_0000032005
	Chain	314 – 504	191	13S globulin seed storage protein 2 basic chain By similarity		PRO_0000032006
Amino acid modifications
	Disulfide bond	46 ↔ 79		By similarity
	Disulfide bond	122 ↔ 320		Interchain (between acidic and basic chains) Potential

Sequences

	Sequence		Length	Mass (Da)
	O23880-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: CDCA322394A28194	FASTA	504	57,043
	10 20 30 40 50 60 MSTKLILSFS LCLMVLSCSA QLWPWQKGQG SRPHHGRQQH QFQHQCDIQR LTASEPSRRV 70 80 90 100 110 120 RSEAGVTEIW DHDTPEFRCT GFVAVRVVIQ PGGLLLPSYS NAPYITFVEQ GRGVQGVVIP 130 140 150 160 170 180 GCPETFQSDS EFEYPQSQRG RHSRQSESEE ESSRGDQHQK IFRIREGDVI PSPAGVVQWT 190 200 210 220 230 240 HNDGNDDLIS VTLLDANSYH KQLDENVRSF FLAGQSQRET REEGSDRQSR ESDDDEALLG 250 260 270 280 290 300 ANILSGFQDE ILHELFRDVD RETISKLRGE NDQRGFIVQA QDLKLRVPQD FEEEYERERG 310 320 330 340 350 360 DRRRGQGGSG RSNGVEQGFC NLKFRRNFNT PTNTYVFNPR AGRINTVNSN SLPILEFLQL 370 380 390 400 410 420 SAQHVVLYKN AIIGPRWNLN AHSALYVTRG EGRVQVVGDE GKSVFDDKVQ RGQILVVPQG 430 440 450 460 470 480 FAVVLKAGRE GLEWVELKNS GNAITSPIGG RTSVLRAIPV EVLANSYDIS TKEAYKLKNG 490 500 RQEVEVFRPF QSRDEKERER FSIV

References

[1]

"Expression, cloning, and immunological analysis of buckwheat (Fagopyrum esculentum Moench) seed storage proteins." Fujino K., Funatsuki H., Inada M., Shimono Y., Kikuta Y. J. Agric. Food Chem. 49:1825-1829(2001) [PubMed: 11308332] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Kitayuki. Tissue: Immature seed.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D87982 mRNA. Translation: BAA21760.1.
PIR	T10698.
3D structure databases
HSSP	HSSP built from PDB template 1FXZ based on UniProtKB P04776.
ModBase	Search...
Family and domain databases
InterPro	IPR006045. Cupin_1. IPR014710. RmlC-like_jellyroll. IPR006044. Seedstore11s_pln. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF00190. Cupin_1. 2 hits. [Graphical view]
PRINTS	PR00439. 11SGLOBULIN.
ProtoNet	Search...

Entry information

Entry name

13S2_FAGES

Accession

Primary (citable) accession number: O23880

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	January 1, 1998
Last modified:	November 25, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)