Reviewed, UniProtKB/Swiss-Prot O24915 (MTNN_HELPY)

Names and origin

Protein names

Recommended name:
    MTA/SAH nucleosidase
    EC=3.2.2.9
Alternative name(s):
    5'-methylthioadenosine nucleosidase
    S-adenosylhomocysteine nucleosidase

Gene names

Name:	mtnN
Synonyms:	mtn
Ordered Locus Names:	HP_0089

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Responsible for cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH) By similarity.
Catalytic activity	S-adenosyl-L-homocysteine + H(2)O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. S-methyl-5'-thioadenosine + H(2)O = S-methyl-5-thio-D-ribose + adenine. Methylthioadenosine + H(2)O = adenine + 5-methylthio-D-ribose.
Sequence similarities	Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 231	231	MTA/SAH nucleosidase
Experimental info
	Sequence conflict	25	1	D → G in AAC64855. Ref.2
	Sequence conflict	109	1	D → N in AAC64855. Ref.2
	Sequence conflict	134	1	E → K in AAC64855. Ref.2
	Sequence conflict	142	1	V → A in AAC64855. Ref.2
	Sequence conflict	147	1	V → L in AAC64855. Ref.2
	Sequence conflict	230	1	E → R in AAC64855. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O24915-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 8704A43F0189CD08	FASTA	231	25,209
	10 20 30 40 50 60 MVQKIGILGA MREEITPILE LFGVDFEEIP LGGNVFHKGV YHNKEIIVAY SKIGKVHSTL 70 80 90 100 110 120 TTTSMILAFG VQKVLFSGVA GSLVKDLKIN DLLVAIQLVQ HDVDLSAFDH PLGFIPESAI 130 140 150 160 170 180 FIETSESLNA LAKEVANEQH IVLKEGVIAS GDQFVHSKER KEFLVSEFKA SAVEMEGASV 190 200 210 220 230 AFVCQKFGVP CCVLRSISDN ADEEANMSFD AFLEKSAQTS AKFLKSMVDE L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[2]	Beier D., Scarlato V. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 4867 / CCUG 17874 / NCTC 11638.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000511 Genomic DNA. Translation: AAD07157.1. AF009177 Genomic DNA. Translation: AAC64855.1. Different initiation.
PIR	A64531.
RefSeq	NP_206889.1.
3D structure databases
HSSP	HSSP built from PDB template 1NC3 based on UniProtKB P24247.
ModBase	Search...
Genome annotation databases
GeneID	900251.
GenomeReviews	Gene locus HP_0089 in contig AE000511_GR.
KEGG	hpy:HP0089.
NMPDR	fig|85962.1.peg.87.
TIGR	HP_0089.
Phylogenomic databases
HOGENOM	O24915.
Family and domain databases
InterPro	IPR010049. MTA_SAH_Nsdase. IPR000845. PNP_UDP. [Graphical view]
PANTHER	PTHR21234. PNP_UDP. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01704. MTA/SAH-Nsdase. 1 hit.
ProDom	O24915. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MTNN_HELPY

Accession

Primary (citable) accession number: O24915
Secondary accession number(s): O32636

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	July 22, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)