Reviewed, UniProtKB/Swiss-Prot O24990 (FABI_HELPY)

Names and origin

Protein names

Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH]
    EC=1.3.1.9
Alternative name(s):
    NADH-dependent enoyl-ACP reductase

Gene names

Name:	fabI
Ordered Locus Names:	HP_0195

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Acyl-[acyl-carrier-protein] + NAD(+) = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subcellular location	Cell inner membrane; Peripheral membrane proteinBy similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 275

275

Enoyl-[acyl-carrier-protein] reductase [NADH]

Regions

Nucleotide binding

10 – 36

27

NAD By similarity

Sites

Binding site

155

1

Substrate By similarity

Secondary structure

1

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275

Helix Strand Turn

Details...

Turn

3 – 6

4

Beta strand

8 – 12

5

Helix

20 – 29

10

Turn

30 – 32

3

Beta strand

34 – 41

8

Turn

42 – 44

3

Helix

45 – 54

10

Beta strand

60 – 62

3

Helix

68 – 81

14

Beta strand

85 – 90

6

Helix

97 – 100

4

Helix

104 – 106

3

Helix

109 – 119

11

Helix

121 – 130

10

Helix

131 – 133

3

Beta strand

134 – 144

11

Helix

146 – 148

3

Helix

156 – 177

22

Turn

178 – 180

3

Beta strand

182 – 188

7

Helix

196 – 198

3

Helix

202 – 212

11

Helix

221 – 232

12

Helix

234 – 236

3

Beta strand

243 – 247

5

Helix

250 – 252

3

Beta strand

253 – 255

3

Turn

262 – 265

4

Helix

268 – 271

4

Sequences

	Sequence		Length	Mass (Da)
	O24990-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 1894A5AA2105895A	FASTA	275	29,981
	10 20 30 40 50 60 MGFLKGKKGL IVGVANNKSI AYGIAQSCFN QGATLAFTYL NESLEKRVRP IAQELNSPYV 70 80 90 100 110 120 YELDVSKEEH FKSLYNSVKK DLGSLDFIVH SVAFAPKEAL EGSLLETSKS AFNTAMEISV 130 140 150 160 170 180 YSLIELTNTL KPLLNNGASV LTLSYLGSTK YMAHYNVMGL AKAALESAVR YLAVDLGKHH 190 200 210 220 230 240 IRVNALSAGP IRTLASSGIA DFRMILKWNE INAPLRKNVS LEEVGNAGMY LLSSLSSGVS 250 260 270 GEVHFVDAGY HVMGMGAVEE KDNKATLLWD LHKEQ

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000511 Genomic DNA. Translation: AAD07262.1.

PIR

C64544.

RefSeq

NP_206994.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PD3	X-ray	2.50	A/B/C/D	1-275	[»]
2PD4	X-ray	2.30	A/B/C/D	1-275	[»]

ModBase

Search...

Genome annotation databases

GeneID

899156.

GenomeReviews

Gene locus HP_0195 in contig AE000511_GR.

KEGG

hpy:HP0195.

NMPDR

fig|85962.1.peg.192.

TIGR

HP_0195.

Phylogenomic databases

HOGENOM

O24990.

Family and domain databases

InterPro

IPR002198. DHase_sc/Rdtase_SDR.
IPR014358. Enoyl-ACP_rdct.
IPR002347. Glc/ribitol_DHase.
IPR016040. NAD(P)-bd.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR19410. ADH_short_C2. 1 hit.
PTHR19410:SF12. Enoyl-ACP_rdct. 1 hit.

PRINTS

PR00081. GDHRDH.

ProDom

O24990.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

ProtoNet

Search...

Entry information

Entry name

FABI_HELPY

Accession

Primary (citable) accession number: O24990

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	September 2, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)