Reviewed, UniProtKB/Swiss-Prot O25001 (HCPA_HELPY)

Names and origin

Protein names

Recommended name:
    Beta-lactamase hcpA
    EC=3.5.2.6
Alternative name(s):
    Cysteine-rich 28 kDa protein

Gene names

Name:	hcpA
Ordered Locus Names:	HP_0211

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Slowly hydrolyzes 6-aminopenicillinic acid and 7-aminocephalosporanic acid (ACA) derivatives. May be involved in the synthesis of the cell wall peptidoglycan.
Catalytic activity	A beta-lactam + H(2)O = a substituted beta-amino acid.
Enzyme regulation	Inhibited by cloxacillin and oxacillin but not by ACA derivatives or metal chelators.
Subcellular location	Secreted.
Miscellaneous	Antibodies against hcpA are present in sera from human patients infected by Helicobacter pylori.
Sequence similarities	Belongs to the hcp beta-lactamase family. Contains 5 TPR repeats.
Mass spectrometry	Molecular weight is 25613±1.6 Da from positions 26 - 250. Determined by MALDI. Ref.4

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Secreted
Domain	Repeat Signal TPR repeat
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 250	225	Beta-lactamase hcpA
Regions
	Repeat	29 – 62	34	TPR 1
	Repeat	67 – 98	32	TPR 2
	Repeat	100 – 133	34	TPR 3
	Repeat	134 – 169	36	TPR 4
	Repeat	170 – 202	33	TPR 5
Amino acid modifications
	Disulfide bond	56 ↔ 64
	Disulfide bond	92 ↔ 100
	Disulfide bond	128 ↔ 136
	Disulfide bond	164 ↔ 172
	Disulfide bond	196 ↔ 204
	Disulfide bond	232 ↔ 240		Potential
Natural variations
	Natural variant	4	1	N → S in strain: ATCC 49503.
	Natural variant	9	1	L → F in strain: ATCC 49503.
	Natural variant	11	1	G → W in strain: ATCC 49503.
	Natural variant	17	1	T → A in strain: ATCC 49503.
	Natural variant	91	1	G → S in strain: ATCC 49503.
	Natural variant	194	1	K → Q in strain: ATCC 49503.
	Natural variant	199	1	K → N in strain: ATCC 49503.
	Natural variant	235	1	S → G in strain: ATCC 49503.

Sequences

	Sequence		Length	Mass (Da)
	O25001-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A375FF12EB795355	FASTA	250	27,366
	10 20 30 40 50 60 MLGNVKKTLF GVLCLGTLCL RGLMAEPDAK ELVNLGIESA KKQDFAQAKT HFEKACELKN 70 80 90 100 110 120 GFGCVFLGAF YEEGKGVGKD LKKAIQFYTK GCELNDGYGC NLLGNLYYNG QGVSKDAKKA 130 140 150 160 170 180 SQYYSKACDL NHAEGCMVLG SLHHYGVGTP KDLRKALDLY EKACDLKDSP GCINAGYIYS 190 200 210 220 230 240 VTKNFKEAIV RYSKACELKD GRGCYNLGVM QYNAQGTAKD EKQAVENFKK GCKSSVKEAC 250 DALKELKIEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of Helicobacter pylori dapE and construction of a conditionally lethal dapE mutant." Karita M., Etterbeek M.L., Forsyth M.H., Tummuru M.K.R., Blaser M.J. Infect. Immun. 65:4158-4164(1997) [PubMed: 9317022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49503 / 60190.
[2]	"Extracellular release of antigenic proteins by Helicobacter pylori." Cao P., McClain M.S., Forsyth M.H., Cover T.L. Infect. Immun. 66:2984-2986(1998) [PubMed: 9596777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION. Strain: ATCC 49503 / 60190.
[3]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[4]	"The cysteine-rich protein A from Helicobacter pylori is a beta-lactamase." Mittl P.R.E., Luethy L., Hunziker P., Gruetter M.G. J. Biol. Chem. 275:17693-17699(2000) [PubMed: 10748053] [Abstract] Cited for: FUNCTION, ENZYME REGULATION, MASS SPECTROMETRY, DISULFIDE BONDS.
[5]	"Detection of high titers of antibody against Helicobacter cysteine-rich proteins A, B, C, and E in Helicobacter pylori-infected individuals." Mittl P.R.E., Luethy L., Reinhardt C., Joller H. Clin. Diagn. Lab. Immunol. 10:542-545(2003) [PubMed: 12853383] [Abstract] Cited for: ANTIGENICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF008565 Genomic DNA. Translation: AAB63298.1. AF053708 Genomic DNA. Translation: AAC24210.1. AE000511 Genomic DNA. Translation: AAD07279.1.
PIR	C64546.
RefSeq	NP_207009.1.
3D structure databases
HSSP	HSSP built from PDB template 1KLX based on UniProtKB O25103.
ModBase	Search...
Genome annotation databases
GeneID	899162.
GenomeReviews	Gene locus HP_0211 in contig AE000511_GR.
KEGG	hpy:HP0211.
NMPDR	fig|85962.1.peg.207.
TIGR	HP_0211.
Phylogenomic databases
HOGENOM	O25001.
Family and domain databases
InterPro	IPR006597. Sel1_like. IPR011990. TPR-like_helical. IPR013026. TPR_region. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
Pfam	PF08238. Sel1. 6 hits. [Graphical view]
SMART	SM00671. SEL1. 6 hits. [Graphical view]
PROSITE	PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view]
ProDom	O25001. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	O25001.
ProtoNet	Search...

Entry information

Entry name

HCPA_HELPY

Accession

Primary (citable) accession number: O25001
Secondary accession number(s): O32634, O85223

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	January 1, 1998
Last modified:	September 2, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)