Reviewed, UniProtKB/Swiss-Prot O28029 (SYA_ARCFU)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	AF_2255

Organism

Archaeoglobus fulgidus [Complete proteome] [HAMAP]

Taxonomic identifier

2234 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Archaeoglobus

Protein attributes

Sequence length	906 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 906	906	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	O28029-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 2AD39B7B65C72C8B	FASTA	906	102,536
	10 20 30 40 50 60 MTLDEEYLDI TFLTENGFVR KRCPKCGKHF WTADPEREIC GDPPCESYSF IGNPVFKKPF 70 80 90 100 110 120 ELDEMREYYL NFFERRGHGR IERYPVVARW RTDIYLTIAS IADFQPFVTS GVAPPPANPL 130 140 150 160 170 180 TISQPCIRLD DLDSVGRTGR HLTLFEMMAH HAFNYPGKEI YWKNETVAYC TELLNELGVK 190 200 210 220 230 240 KEDIVYKEEP WAGGGNAGPC LEAIVGGLEV ATLVFMNLEE HPEGDIEIKG ARYRKMDNYI 250 260 270 280 290 300 VDTGYGLERF VWASKGTPTV YDAIFPEVVD TIIDNSNVSF NREDERVRRI VAESSKLAGI 310 320 330 340 350 360 MGELRGERLN QLRKSVADTV GVSVEELEGI VVPLEKVYSL ADHTRCILFM LGDGLVPSNA 370 380 390 400 410 420 GAGYLARLMI RRSLRLAEEL ELGLDLYDLV EMHKKILGFE FDVPLSTVQE ILELEKERYR 430 440 450 460 470 480 TTVSKGTRLV ERLVERKKKL EKDDLIELYD SHGIPVELAV GIAAEKGAEV EMPKDIYAEL 490 500 510 520 530 540 AKRHSKAEKV QEKKITLQNE YPATEKLYYD DPTLLEFEAE VIGVEGDFVI LNRSAFYPES 550 560 570 580 590 600 GGQDNDVGYL IANGGKFEVV DVLEADGVVL HVVKGAKPEV GTKVKGVIDS DVRWRHMRHH 610 620 630 640 650 660 SATHVLLYSL QKVLGNHVWQ AGARKEFSKA RLDVTHFRRP SEEEIKEIEM LANREILANK 670 680 690 700 710 720 PIKWEWMDRI EAERKFGFRL YQGGVPPGRK IRVVQVGDDV QACGGTHCRS TGEIGMLKIL 730 740 750 760 770 780 KVESIQDGVI RFEFAAGEAA IEAVEEMERL LREASSILRV EPAKLPKTVE RFFEEWKDQR 790 800 810 820 830 840 KEIERLKSVI ADLWADILME RAEEFDSMKV VAEVVDADMQ ALQKLAERLA EKGAVGCLMA 850 860 870 880 890 900 KGEGKVFVVT FSGQKYDARE LLREIGRVAK GSGGGRKDVA QGAVQQLLDR EEMLDVIFRF LSEHEG

References

[1]

"The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus." Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A., Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L., Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D., Quackenbush J., Lee N.H., Sutton G.G. , Gill S.R., Kirkness E.F., Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N., Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R., Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D., Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P., Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Nature 390:364-370(1997) [PubMed: 9389475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000782 Genomic DNA. Translation: AAB89002.1.
PIR	G69531.
RefSeq	NP_071080.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1485486.
GenomeReviews	Gene locus AF_2255 in contig AE000782_GR.
KEGG	afu:AF2255.
NMPDR	fig|224325.1.peg.2241.
TIGR	AF_2255.
Phylogenomic databases
HOGENOM	O28029.
Enzyme and pathway databases
BioCyc	AFUL224325:AF_2255-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	O28029. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_ARCFU

Accession

Primary (citable) accession number: O28029

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	July 22, 2008
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)