Reviewed, UniProtKB/Swiss-Prot O30868 (MTH2_HAEAE)

Names and origin

Protein names

Recommended name:
    Modification methylase HaeII
      Short name=M.HaeII
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase HaeII

Gene names

Name:	haeIIM
Synonyms:	dcmA

Organism

Haemophilus aegyptius

Taxonomic identifier

197575 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence RGCGCY, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HaeII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Modification methylase HaeII		PRO_0000087877
Sites
	Active site	73	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O30868-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: CA879CF66731D434	FASTA	318	35,670
	10 20 30 40 50 60 MKEYKTIDLF AGIGGIRLGF EAFGCKNVFS SEWDKYAQSM YEVNFGEKPF GDINDISPSD 70 80 90 100 110 120 IPDHDILLAG FPCQPFSIAG KGLGFADTRG TLFFNIEAIL KAKKPKAFLL ENVKRLTTHD 130 140 150 160 170 180 NGNTFKVIND KLNKLGYTVY HKVLNTLDFG LPQKRERIYI VGFLDKLHFE FPKPIGKYEE 190 200 210 220 230 240 LSNILESDDV VPNNYFLSDE LKRKRLSSIT KDVPYPSIWH ENISGNVSAL PYSCALSVGG 250 260 270 280 290 300 SYNYLVVNGV RRLTGPEMLR LQGFPDTFEI NIPYSQVRKV AGNSVSVPVI RAIAGSMINS 310 LNMATRKIYQ AIQLSLLD

References

[1]	"Sequence similarities between the genes encoding the S.NgoI and HaeII restriction/modification systems." Stein D.C., Gunn J.S., Piekarowicz A. Biol. Chem. 379:575-578(1998) [PubMed: 9628358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11116 / CCUG 25716 / NCTC 8502.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF019752 Genomic DNA. Translation: AAB70829.1.
3D structure databases
HSSP	HSSP built from PDB template 6MHT based on UniProtKB P05102.
ModBase	Search...
Protein family/group databases
REBASE	3409. M.HaeII.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 2 hits. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTH2_HAEAE

Accession

Primary (citable) accession number: O30868

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 1, 1998
Last modified:	November 25, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)