Reviewed, UniProtKB/Swiss-Prot P39130 (LPLD_BACSU)

Names and origin

Protein names

Recommended name:
    Putative glucosidase lplD
    EC=3.2.1.-

Gene names

Name:	lplD
Ordered Locus Names:	BSU07130

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Cofactor	NAD By similarity. Divalent metal ion By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 4 family.

Ontologies

Keywords
Ligand	Manganese Metal-binding NAD
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Putative glucosidase lplD		PRO_0000169862
Regions
	Nucleotide binding	10 – 72	63	NAD By similarity
Sites
	Active site	174	1	Proton donor By similarity
	Metal binding	173	1	Manganese By similarity
	Metal binding	210	1	Manganese By similarity
	Binding site	151	1	Substrate By similarity
Experimental info
	Sequence conflict	66	1	R → T in AAA22577. Ref.1
	Sequence conflict	154	1	S → Y in AAA22577. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P39130-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 691FEC5C371B9F5B	FASTA	446	49,487
	10 20 30 40 50 60 MFHISTLDQI KIAYIGGGSQ GWARSLMSDL SIDERMSGTV ALYDLDFEAA QKNEVIGNHS 70 80 90 100 110 120 GNGRWRYEAV STLKKALSAA DIVIISILPG SLDDMEVDVH LPERCGIYQS VGDTVGPGGI 130 140 150 160 170 180 IRGLRAVPIF AEIARAIRDY APESWVINYT NPMSVCTRVL YKVFPGIKAI GCCHEVFGTQ 190 200 210 220 230 240 KLLAEMVTER LGIEVPRRED IRVNVLGINH FTWITKASYR HIDLLPIFRE FSAHYGESGY 250 260 270 280 290 300 ELEGECWRDS VFCSAHRVAF DLFETYGAIP AAGDRHLAEF LPGPYLKQPE VWKFHLTPIS 310 320 330 340 350 360 FRKQDRAEKR QETERLIVQQ RGVAEKASGE EGVNIIAALL GLGELVTNVN MPNQGQVLNL 370 380 390 400 410 420 PIQAIVETNA FITRNRVQPI LSGALPKGVE MLAARHISNQ EAVADAGLTK DTGLAFQAFL 430 440 NDPLVQIDRS DAEQLFNDML QCIMQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete lpl cluster of Bacillus subtilis." Gomez A., Ramon D., Sanz P. Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / Marburg.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L19165 Genomic DNA. Translation: AAA22577.1. Z99107 Genomic DNA. Translation: CAB12532.1.
PIR	C69653.
RefSeq	NP_388594.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	938770.
GenomeReviews	Gene locus BSU07130 in contig AL009126_GR.
KEGG	bsu:BSU07130.
NMPDR	fig|224308.1.peg.713.
Organism-specific databases
SubtiList	BG10858. lplD. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P39130.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU0713-MON.
Family and domain databases
InterPro	IPR001088. Glyco_hydro_4. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit.
Pfam	PF02056. Glyco_hydro_4. 1 hit. [Graphical view]
PRINTS	PR00732. GLHYDRLASE4.
ProDom	PD006892. Glyco_hydro_4. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01324. GLYCOSYL_HYDROL_F4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPLD_BACSU

Accession

Primary (citable) accession number: P39130
Secondary accession number(s): O31532

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 15, 1998
Last modified:	November 25, 2008
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)