Reviewed,
UniProtKB/Swiss-Prot O32137 (ALLB_BACSU)
Last modified
November 25, 2008.
Version 65.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (3) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Allantoinase EC=3.5.2.5 Alternative name(s): Allantoin-utilizing enzyme | ||||||
| Gene names |
| ||||||
| Organism | Bacillus subtilis [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 1423 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 446 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Catalyzes the conversion of allantoin (5-ureidohydantoin) to allantoic acid by hydrolytic cleavage of the five-member hydantoin ring. Ref.2 |
| Catalytic activity | (S)-allantoin + H(2)O = allantoate. HAMAP MF_01645 |
| Cofactor | Binds 2 zinc ions per subunit By similarity. |
| Pathway | Nitrogen metabolism; (S)-allantoin degradation; allantoate from (S)-allantoin: step 1/1. HAMAP MF_01645 |
| Subunit structure | Homotetramer By similarity. |
| Induction | Expression is very low in excess nitrogen (glutamate plus ammonia) and is induced during limiting-nitrogen conditions (glutamate). Expression is further induced when allantoin or allantoate are added during limiting-nitrogen conditions. Ref.2 |
| Post-translational modification | Carbamylation allows a single lysine to coordinate two zinc ions By similarity. |
| Sequence similarities | Belongs to the DHOase family. Allantoinase subfamily. |
Ontologies
Keywords | |
|---|---|
| Biological process | Purine metabolism |
| Ligand | Metal-binding Zinc |
| Molecular function | Hydrolase |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | allantoin catabolic process Inferred from electronic annotation. Source: HAMAP purine base metabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | allantoinase activity Inferred from electronic annotation. Source: HAMAP zinc ion bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 446 | 446 | Allantoinase HAMAP MF_01645 | PRO_0000165940 | |||||
Sites | |||||||||
| Metal binding | 60 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 62 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 147 | 1 | Zinc 1; via carbamate group By similarity | ||||||
| Metal binding | 147 | 1 | Zinc 2; via carbamate group By similarity | ||||||
| Metal binding | 183 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 239 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 312 | 1 | Zinc 1 By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 147 | 1 | N6-carboxylysine By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.  Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| [2] | "Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator." Schultz A.C., Nygaard P., Saxild H.H. J. Bacteriol. 183:3293-3302(2001) [PubMed: 11344136] [Abstract] Cited for: FUNCTION, INDUCTION. Strain: 168. |
Cross-references
Sequence databases | |
|---|---|
| Z99120 Genomic DNA. Translation: CAB15231.1. | |
| PIR | C70016. |
| RefSeq | NP_391121.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1GKR based on UniProtKB P81006. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 936662. |
| GenomeReviews | Gene locus BSU32410 in contig AL009126_GR. |
| KEGG | bsu:BSU32410. |
| NMPDR | fig|224308.1.peg.3247. |
Organism-specific databases | |
| SubtiList | BG13982. pucH. [Micado] |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | O32137. |
Enzyme and pathway databases | |
| BioCyc | BSUB224308:BSU3238-MON. |
Family and domain databases | |
| HAMAP | MF_01645. [Tree] |
| InterPro | IPR017593. Allantoinase. IPR006680. Amidohydro_1. [Graphical view] |
| Pfam | PF01979. Amidohydro_1. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ALLB_BACSU | ||||||||
| Accession | Primary (citable) accession number: O32137 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
