Reviewed, UniProtKB/Swiss-Prot O33843 (BGLA_THENE)

Names and origin

Protein names

Recommended name:
    Beta-glucosidase A
    EC=3.2.1.21
Alternative name(s):
    Gentiobiase
    Cellobiase
    Beta-D-glucoside glucohydrolase

Gene names

Name:	bglA
Synonyms:	gghA

Organism

Thermotoga neapolitana

Taxonomic identifier

2337 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This enzyme has broad substrate specificity. Acts more readily on cellotetraose than on cellobiose (16-fold greater Kcat/Km).
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose.
Pathway	Glycan metabolism; cellulose degradation.
Subunit structure	Monomer.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.
Biophysicochemical properties	Kinetic parameters: KM=28.6 mM for cellobiose KM=4.55 mM for cellotriose KM=2.15 mM for cellotetraose pH dependence: Optimum pH is 6.5. Temperature dependence: Optimum temperature is 95 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 444	444	Beta-glucosidase A
Sites
	Active site	164	1	Proton donor Potential
	Active site	349	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	O33843-1 [UniParc]. Last modified July 11, 2001. Version 2. Checksum: 43B7D49A20A8CE01	FASTA	444	51,493
	10 20 30 40 50 60 MKKFPEGFLW GVATASYQIE GSPLADGAGM SIWHTFSHTP GNVKNGDTGD VACDHYNRWK 70 80 90 100 110 120 EDIEIIEKIG AKAYRFSISW PRILPEGTGK VNQKGLDFYN RIIDTLLEKN ITPFITIYHW 130 140 150 160 170 180 DLPFSLQLKG GWANRDIADW FAEYSRVLFE NFGDRVKHWI TLNEPWVVAI VGHLYGVHAP 190 200 210 220 230 240 GMKDIYVAFH TVHNLLRAHA KSVKVFRETV KDGKIGIVFN NGYFEPASER EEDIRAARFM 250 260 270 280 290 300 HQFNNYPLFL NPIYRGEYPD LVLEFAREYL PRNYEDDMEE IKQEIDFVGL NYYSGHMVKY 310 320 330 340 350 360 DPNSPARVSF VERNLPKTAM GWEIVPEGIY WILKGVKEEY NPQEVYITEN GAAFDDVVSE 370 380 390 400 410 420 GGKVHDQNRI DYLRAHIEQV WRAIQDGVPL KGYFVWSLLD NFEWAEGYSK RFGIVYVDYN 430 440 TQKRIIKDSG YWYSNGIKNN GLTD

References

[1]

"Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana." Yernool D.A., McCarthy J.K., Eveleigh D.E., Bok J.-D. J. Bacteriol. 182:5172-5179(2000) [PubMed: 10960102] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-21, CHARACTERIZATION. Strain: ATCC 49049 / DSM 4359 / NS-E.

[2]

Zverlov V. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-425. Strain: Z2706-MC24.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF039487 Genomic DNA. Translation: AAB95492.2. Z97212 Genomic DNA. Translation: CAB10165.1.
3D structure databases
HSSP	HSSP built from PDB template 1OD0 based on UniProtKB Q08638.
SMR	O33843. Positions 1-444.
ModBase	Search...
Family and domain databases
InterPro	IPR001360. Glyco_hydro_1. IPR017736. Glyco_hydro_1_beta-glucosidase. IPR013781. Glyco_hydro_sub_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProDom	O33843. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

BGLA_THENE

Accession

Primary (citable) accession number: O33843
Secondary accession number(s): O52505

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 11, 2001
Last modified:	September 2, 2008
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)