Reviewed, UniProtKB/Swiss-Prot O34526 (SYA_BACSU)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BSU27410

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 878	878	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	O34526-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 10C0FDDE9DF5E4AD	FASTA	878	97,280
	10 20 30 40 50 60 MKHLTSAEVR QMFLDFFKEK GHAVEPSASL VPHEDPSLLW INSGVATLKK YFDGRVVPEN 70 80 90 100 110 120 PRIVNAQKAI RTNDIENVGK TARHHTFFEM LGNFSIGDYF KEEAITWAWE FLTSDKWIGF 130 140 150 160 170 180 DKELLSVTVH PEDEEAYEFW AKKIGIPEER IIRLEGNFWD IGEGPSGPNT EIFYDRGEAY 190 200 210 220 230 240 GNDPEDPELY PGGENDRYLE VWNLVFSEFN HNPDGTYTPL PKKNIDTGMG LERMVSVIQN 250 260 270 280 290 300 VPTNFDTDLF VPIIKATESI SGETYGKDNV KDTAFKVIAD HIRTVAFAVS DGALPSNEGR 310 320 330 340 350 360 GYVLRRLLRR AVRYAKTINI HRPFMFDLVP VVAEIMADFY PEVKEKADFI AKVIKTEEER 370 380 390 400 410 420 FHETLNEGLA ILSEMIKKEK DKGSSVISGA DVFKLYDTYG FPVELTEEYA EDENMTVDHE 430 440 450 460 470 480 GFEEEMNQQR ERARNARQDV GSMQVQGGAL RDVTVESTFV GYSQTKADAN IIVLLQDGQL 490 500 510 520 530 540 IEEAHEGESV QIILDETPFY AESGGQIGDK GYLRSEQAVV RIKDVQKAPN GQHVHEGVVE 550 560 570 580 590 600 SGTVQKGLHV TAEVEDHMRS GVIKNHTATH LLHQALKDVL GTHVNQAGSL VTENRLRFDF 610 620 630 640 650 660 SHFGQVTKEE LEQIERIVNE KIWASIPVSI DLKPIAEAKE MGAMALFGEK YGDIVRVVQV 670 680 690 700 710 720 GDYSLELCGG CHVRNTAEIG LFKIVSESGI GAGTRRIEAV TGQGAYVEMN SQISVLKQTA 730 740 750 760 770 780 DELKTNIKEV PKRVAALQAE LKDAQRENES LLAKLGNVEA GAILSKVKEV DGVNVLAAKV 790 800 810 820 830 840 NAKDMNHLRT MVDELKAKLG SAVIVLGAVQ NDKVNISAGV TKDLIEKGLH AGKLVKQAAE 850 860 870 VCGGGGGGRP DMAQAGGKQP EKLEEALASV EDWVKSVL

References

[1]

"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z99117 Genomic DNA. Translation: CAB14682.1.
PIR	A69584.
RefSeq	NP_390618.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	939643.
GenomeReviews	Gene locus BSU27410 in contig AL009126_GR.
KEGG	bsu:BSU27410.
NMPDR	fig|224308.1.peg.2743.
Organism-specific databases
SubtiList	BG12563. alaS. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	O34526.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2736-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	O34526. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_BACSU

Accession

Primary (citable) accession number: O34526

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	July 22, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)