Reviewed, UniProtKB/Swiss-Prot O35632 (HYAL2_MOUSE)

Names and origin

Protein names

Recommended name:
    Hyaluronidase-2
      Short name=Hyal-2
    EC=3.2.1.35
Alternative name(s):
    Hyaluronoglucosaminidase-2
      Short name=LUCA-2

Gene names

Name:

Hyal2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R By similarity.
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subunit structure	Interacts with MST1R By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchorBy similarity.
Tissue specificity	Widely expressed. In the brain, expressed during embryonic stages but expression decreases after birth and is barely detectable in adult brain. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain.
Caution	Was originally thought to be lysosomal.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	EGF-like domain Signal
Molecular function	Glycosidase Hydrolase Receptor
PTM	GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: InterPro receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 448	428	Hyaluronidase-2		PRO_0000012101
	Propeptide	449 – 473	25	Removed in mature form Potential		PRO_0000012102
Regions
	Domain	361 – 439	79	EGF-like
Sites
	Active site	135	1	Proton donor By similarity
Amino acid modifications
	Lipidation	448	1	GPI-anchor amidated asparagine Potential
	Glycosylation	74	1	N-linked (GlcNAc...) Potential
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	357	1	N-linked (GlcNAc...) Potential
	Glycosylation	390	1	N-linked (GlcNAc...) Potential
	Glycosylation	448	1	N-linked (GlcNAc...) Potential
	Disulfide bond	47 ↔ 340		By similarity
	Disulfide bond	211 ↔ 227		By similarity
	Disulfide bond	365 ↔ 376		By similarity
	Disulfide bond	370 ↔ 427		By similarity
	Disulfide bond	429 ↔ 438		By similarity
Experimental info
	Sequence conflict	26 – 27	2	TA → KP in CAA03888 and CAA03889. Ref.1
	Sequence conflict	193 – 199	7	YVKAVRP → LRQGSQT in CAA03888. Ref.1
	Sequence conflict	250	1	Missing in CAA03888. Ref.1
	Sequence conflict	269 – 272	4	SFRV → RFGG in CAA03888. Ref.1
	Sequence conflict	355	1	I → V in AAK28481. Ref.2
	Sequence conflict	383	1	A → V in CAA03888 and CAA03889. Ref.1
	Sequence conflict	416 – 422	7	ADLNYLQ → RDRQLPE in CAA03888. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O35632-1 [UniParc]. Last modified November 15, 2002. Version 2. Checksum: DD433C9FFCF8147C	FASTA	473	53,618
	10 20 30 40 50 60 MRAGLGPIIT LALVLEVAWA GELKPTAPPI FTGRPFVVAW NVPTQECAPR HKVPLDLRAF 70 80 90 100 110 120 DVKATPNEGF FNQNITTFYY DRLGLYPRFD AAGTSVHGGV PQNGSLCAHL PMLKESVERY 130 140 150 160 170 180 IQTQEPGGLA VIDWEEWRPV WVRNWQEKDV YRQSSRQLVA SRHPDWPSDR VMKQAQYEFE 190 200 210 220 230 240 FAARQFMLNT LRYVKAVRPQ HLWGFYLFPD CYNHDYVQNW ESYTGRCPDV EVARNDQLAW 250 260 270 280 290 300 LWAESTALFP SVYLDETLAS SVHSRNFVSF RVREALRVAH THHANHALPV YVFTRPTYTR 310 320 330 340 350 360 GLTGLSQVDL ISTIGESAAL GSAGVIFWGD SEDASSMETC QYLKNYLTQL LVPYIVNVSW 370 380 390 400 410 420 ATQYCSWTQC HGHGRCVRRN PSANTFLHLN ASSFRLVPGH TPSEPQLRPE GQLSEADLNY 430 440 450 460 470 LQKHFRCQCY LGWGGEQCQR NYKGAAGNAS RAWAGSHLTS LLGLVAVALT WTL

References

[1]	"Structural organization and chromosomal localization of Hyal2, a gene encoding a lysosomal hyaluronidase." Strobl B., Wechselberger C., Beier D., Lepperdinger G. Genomics 53:214-219(1998) [PubMed: 9790770] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY. Strain: 129/SvJ.
[2]	"Candidate tumor suppressor HYAL2 is a glycosylphosphatidylinositol (GPI)-anchored cell-surface receptor for jaagsiekte sheep retrovirus, the envelope protein of which mediates oncogenic transformation." Rai S.K., Duh F.-M., Vigdorovich V., Danilkovitch-Miagkova A., Lerman M.I., Miller A.D. Proc. Natl. Acad. Sci. U.S.A. 98:4443-4448(2001) [PubMed: 11296287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C3H and Czech II.
[3]	"Transforming growth factor-beta1 blocks the enhancement of tumor necrosis factor cytotoxicity by hyaluronidase Hyal-2 in L929 fibroblasts." Chang N.-S. BMC Cell Biol. 3:8-8(2002) [PubMed: 11960552] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Czech II.
[4]	"Hyal2 -- less active, but more versatile?" Lepperdinger G., Mullegger J., Kreil G. Matrix Biol. 20:509-514(2001) [PubMed: 11731268] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ000059 mRNA. Translation: CAA03888.1. AJ000060 Genomic DNA. Translation: CAA03889.1. AF302843 mRNA. Translation: AAK28481.1. AF302844 mRNA. Translation: AAK28482.1. AF422177 mRNA. Translation: AAL17823.1.
RefSeq	NP_034619.2.
UniGene	Mm.465277 Mm.4834
3D structure databases
HSSP	HSSP built from PDB template 1FCQ based on UniProtKB Q08169.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000010047. Mus musculus. [Contig view]
GeneID	15587.
KEGG	mmu:15587.
Organism-specific databases
MGI	MGI:1196334. Hyal2.
Phylogenomic databases
HOGENOM	O35632.
HOVERGEN	O35632.
Gene expression databases
ArrayExpress	O35632.
CleanEx	MM_HYAL2.
GermOnline	ENSMUSG00000010047. Mus musculus.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR006210. EGF. IPR000742. EGF_3. IPR013032. EGF_like_reg_CS. IPR001968. Glyco_hydro_56. IPR017430. Glyco_hydro_56_Hyaluronidase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11769. Glyco_hydro_56. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PIRSF	PIRSF038193. Hyaluronidase. 1 hit.
PRINTS	PR00846. GLHYDRLASE56.
ProDom	PD003549. Glyco_hydro_56. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00181. EGF. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	288580.
SOURCE	Search...

Entry information

Entry name

HYAL2_MOUSE

Accession

Primary (citable) accession number: O35632
Secondary accession number(s): O35631, Q99MS9, Q99MT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	November 15, 2002
Last modified:	November 25, 2008
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)