Reviewed, UniProtKB/Swiss-Prot O42772 (DHSB_MYCGR)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
    EC=1.3.5.1
Alternative name(s):
    Iron-sulfur subunit of complex II
      Short name=Ip

Gene names

Name:

SDH2

Organism

Mycosphaerella graminicola (Septoria tritici)

Taxonomic identifier

54734 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Dothideomycetidae › Capnodiales › Mycosphaerellaceae › Mycosphaerella

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix sideBy similarity.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 297		Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
Regions
	Domain	78 – 157	80	2Fe-2S ferredoxin-type
	Domain	199 – 229	31	4Fe-4S ferredoxin-type
Sites
	Metal binding	117	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	122	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	125	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	137	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	209	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	212	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	215	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	219	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	266	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	272	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	276	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Binding site	224	1	Ubiquinone; shared with DHSD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O42772-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: A50010A24F48F6D4	FASTA	297	33,667
	10 20 30 40 50 60 MALRLATRRF APIAFRRGMA TTIEHTKEPI SATAEALSAS RPPIKETKTS TVKEPQMDAD 70 80 90 100 110 120 AKTKTFHIYR WNPDQPTDKP RMQSYTLDLN KTGPMMLDAL IRIKNEVDPT LTFRRSCREG 130 140 150 160 170 180 ICGSCAMNID GVNTLACLCR IPTDTAKETR IYPLPHTYVV KDLVPDMTQF YKQYKSIKPY 190 200 210 220 230 240 LQRDTAPPDG KENRQSVADR KKLDGLYECI LCACCSTSCP SYWWNSEEYL GPAVLLQSYR 250 260 270 280 290 WINDSRDEKT AQRKDALNNS MSLYRCHTIL NCSRTCPKGL NPALAIAEIK KSMAFTG

References

[1]

"A single amino-acid substitution in the iron-sulphur protein subunit of succinate dehydrogenase determines resistance to carboxin in Mycosphaerella graminicola." Skinner W., Bailey A.M., Renwick A., Keon J.P.R., Gurr S.J., Hargreaves J.A. Curr. Genet. 34:393-398(1998) [PubMed: 9871122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF042062 Genomic DNA. Translation: AAB97419.1.
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P07014.
SMR	O42772. Positions 61-294.
ModBase	Search...
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001450. 4Fe4S_Fe_S_bd. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR004489. Succ_DHase/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
Pfam	PF00111. Fer2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

DHSB_MYCGR

Accession

Primary (citable) accession number: O42772

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	June 1, 1998
Last modified:	September 2, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)