Reviewed, UniProtKB/Swiss-Prot O42971 (APC10_SCHPO)

Names and origin

Protein names

Recommended name:
    Anaphase-promoting complex subunit 10
Alternative name(s):
    20S cyclosome/APC complex protein apc10

Gene names

Name:	apc10
ORF Names:	SPBC1A4.01, SPBC1E8.06

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the anaphase-promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. Acts as a positive regulator of the anaphase promoting complex (APC)-cyclosome. Involved in G1 cell cycle arrest in response to nitrogen starvation. Required for ubiquitination and degradation of the mitotic B-type cyclin, cdc13. Ref.1
Subunit structure	The APC/C is composed of at least 13 subunits: apc1, apc2, nuc2, apc4, apc5, cut9, apc8, apc10, apc11, hcn1, apc13, apc14 and apc15. Interacts with nuc2. Ref.1 Ref.3
Sequence similarities	Belongs to the APC10 family. Contains 1 DOC domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Ubl conjugation pathway
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	activation of anaphase-promoting complex during mitotic cell cycle Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe cell division Inferred from electronic annotation. Source: UniProtKB-KW cyclin catabolic process Inferred from mutant phenotype. Source: GeneDB_SPombe protein ubiquitination Inferred by curator. Source: GeneDB_SPombe
Cellular component	anaphase-promoting complex Ref.1 Inferred from electronic annotation. Source: InterPro cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	Anaphase-promoting complex subunit 10		PRO_0000174015
Regions
	Domain	2 – 187	186	DOC

Sequences

	Sequence		Length	Mass (Da)
	O42971-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 91AB73EA9D2FDE05	FASTA	189	21,474
	10 20 30 40 50 60 MAQIRQEALK KQKSETQKST EGFVDIGNLA QWTCSSEKSG FPIRLVRDDN IDTYWQSDGS 70 80 90 100 110 120 QPHTIHIKFV KRVSIKYVSM YLQYTLDESY TPSTLRISAG TGFQDLEIVT TVQVEEPTGW 130 140 150 160 170 180 VHVPVGDFGR NGLLDVHLIQ IKILANHQSG KDSHVRLIKI YAPEIEQPAI AVDEIPYTSL QFISRNQLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Apc10 and Ste9/Srw1, two regulators of the APC-cyclosome, as well as the CDK inhibitor Rum1 are required for G1 cell-cycle arrest in fission yeast." Kominami K., Seth-Smith H., Toda T. EMBO J. 17:5388-5399(1998) [PubMed: 9736616] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH NUC2.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes." Yoon H.-J., Feoktistova A., Wolfe B.A., Jennings J.L., Link A.J., Gould K.L. Curr. Biol. 12:2048-2054(2002) [PubMed: 12477395] [Abstract] Cited for: SUBUNIT.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAA16839.1. AB012919 Genomic DNA. Translation: BAA32157.1.
PIR	T39849.
RefSeq	NP_595803.1.
3D structure databases
HSSP	HSSP built from PDB template 1GQP based on UniProtKB P53068.
ModBase	Search...
Genome annotation databases
GeneID	2540307.
KEGG	spo:SPBC1A4.01.
NMPDR	fig|4896.1.peg.1669.
Organism-specific databases
GeneDB_Spombe	SPBC1A4.01.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003742-MON.
Gene expression databases
ArrayExpress	O42971.
Family and domain databases
InterPro	IPR004939. APC10. IPR016901. APC10_sub. [Graphical view]
PANTHER	PTHR12936. APC10. 1 hit.
Pfam	PF03256. APC10. 1 hit. [Graphical view]
PIRSF	PIRSF028841. APC10_sub. 1 hit.
PROSITE	PS51284. DOC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APC10_SCHPO

Accession

Primary (citable) accession number: O42971

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	June 1, 1998
Last modified:	November 25, 2008
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)