Reviewed, UniProtKB/Swiss-Prot O43182 (RHG06_HUMAN)

Names and origin

Protein names

Recommended name:
    Rho GTPase-activating protein 6
Alternative name(s):
    Rho-type GTPase-activating protein 6
    Rho-type GTPase-activating protein RhoGAPX-1

Gene names

Name:	ARHGAP6
Synonyms:	RHOGAP6

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	974 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Could regulate the interactions of signaling molecules with the actin cytoskeleton. Promotes continuous elongation of cytoplasmic processes during cell motility and simultaneous retraction of the cell body changing the cell morphology.
Subcellular location	Cytoplasm.
Tissue specificity	Highly expressed in kidney, heart and skeletal muscle followed by retina, lymphoblast, placenta, lung, brain, pancreas and liver.
Miscellaneous	ARHGAP6 gene undergoes X inactivation.
Sequence similarities	Contains 1 Rho-GAP domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	SH3-binding
Molecular function	GTPase activation
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	Rho protein signal transduction Ref.1 Ref.2 Non-traceable author statement. Source: UniProtKB actin filament polymerization Ref.1 Non-traceable author statement. Source: UniProtKB
Cellular component	actin filament Ref.1 Non-traceable author statement. Source: UniProtKB cytoplasm Non-traceable author statement. Source: UniProtKB
Molecular function	Rho GTPase activator activity Ref.1 Ref.2 Non-traceable author statement. Source: UniProtKB SH3/SH2 adaptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 3 (identifier: O43182-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: O43182-2)

The sequence of this isoform differs from the canonical sequence as follows:
     726-765: DLSEEPFDIW...GDIFESSSLR → GNWSLASRRW...SSSLPYLMFL
     766-974: Missing.

Isoform 2 (identifier: O43182-3)

The sequence of this isoform differs from the canonical sequence as follows:
     196-196: E → ELELYDLQILGTKPPMNSDTHRNFDPTATLRNQ
     637-658: SPDMLQSEVSFSVGGRHSSTDS → TSSVLPAAVQACPQYPASMFTP
     659-974: Missing.

Isoform 4 (identifier: O43182-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-203: Missing.

Isoform 5 (identifier: O43182-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-203: Missing.
     726-765: DLSEEPFDIW...GDIFESSSLR → GNWSLASRRW...SSSLPYLMFL
     766-974: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 974	974	Rho GTPase-activating protein 6
Regions
	Domain	401 – 602	202	Rho-GAP
	Motif	342 – 352	11	SH3-binding
Amino acid modifications
	Modified residue	264	1	Phosphoserine
	Modified residue	663	1	Phosphoserine
	Modified residue	673	1	Phosphoserine
	Modified residue	754	1	Phosphoserine
	Modified residue	777	1	Phosphoserine
	Modified residue	928	1	Phosphoserine
	Modified residue	939	1	Phosphoserine
Natural variations
	Alternative sequence	1 – 203	203	Missing in isoform 4 and isoform 5.
	Alternative sequence	196	1	E → ELELYDLQILGTKPPMNSDT HRNFDPTATLRNQ in isoform 2.
	Alternative sequence	637 – 658	22	SPDML…SSTDS → TSSVLPAAVQACPQYPASMF TP in isoform 2.
	Alternative sequence	659 – 974	316	Missing in isoform 2.
	Alternative sequence	726 – 765	40	DLSEE…SSSLR → GNWSLASRRWPKQATLLLLH VAWCGALRTFSSSLPYLMFL in isoform 1 and isoform 5.
	Alternative sequence	766 – 974	209	Missing in isoform 1 and isoform 5.
	Natural variant	791	1	D → E: dbSNP rs1009758.
Experimental info
	Sequence conflict	231	1	A → P in AAF43261 and AAD55087. Ref.1
	Sequence conflict	231	1	A → P Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 3 [UniParc]. Last modified October 3, 2006. Version 3. Checksum: BEE22EB54F019E30	FASTA	974	105,947
	10 20 30 40 50 60 MSAQSLLHSV FSCSSPASSS AASAKGFSKR KLRQTRSLDP ALIGGCGSDE AGAEGSARGA 70 80 90 100 110 120 TAGRLYSPSL PAESLGPRLA SSSRGPPPRA TRLPPPGPLC SSFSTPSTPQ EKSPSGSFHF 130 140 150 160 170 180 DYEVPLGRGG LKKSMAWDLP SVLAGPASSR SASSILCSSG GGPNGIFASP RRWLQQRKFQ 190 200 210 220 230 240 SPPDSRGHPY VVWKSEGDFT WNSMSGRSVR LRSVPIQSLS ELERARLQEV AFYQLQQDCD 250 260 270 280 290 300 LSCQITIPKD GQKRKKSLRK KLDSLGKEKN KDKEFIPQAF GMPLSQVIAN DRAYKLKQDL 310 320 330 340 350 360 QRDEQKDASD FVASLLPFGN KRQNKELSSS NSSLSSTSET PNESTSPNTP EPAPRARRRG 370 380 390 400 410 420 AMSVDSITDL DDNQSRLLEA LQLSLPAEAQ SKKEKARDKK LSLNPIYRQV PRLVDSCCQH 430 440 450 460 470 480 LEKHGLQTVG IFRVGSSKKR VRQLREEFDR GIDVSLEEEH SVHDVAALLK EFLRDMPDPL 490 500 510 520 530 540 LTRELYTAFI NTLLLEPEEQ LGTLQLLIYL LPPCNCDTLH RLLQFLSIVA RHADDNISKD 550 560 570 580 590 600 GQEVTGNKMT SLNLATIFGP NLLHKQKSSD KEFSVQSSAR AEESTAIIAV VQKMIENYEA 610 620 630 640 650 660 LFMVPPDLQN EVLISLLETD PDVVDYLLRR KASQSSSPDM LQSEVSFSVG GRHSSTDSNK 670 680 690 700 710 720 ASSGDISPYD NNSPVLSERS LLAMQEDAAP GGSEKLYRVP GQFMLVGHLS SSKSRESSPG 730 740 750 760 770 780 PRLGKDLSEE PFDIWGTWHS TLKSGSKDPG MTGSSGDIFE SSSLRAGPCS LSQGNLSPNW 790 800 810 820 830 840 PRWQGSPAEL DSDTQGARRT QAAAPATEGR AHPAVSRACS TPHVQVAGKA ERPTARSEQY 850 860 870 880 890 900 LTLSGAHDLS ESELDVAGLQ SRATPQCQRP HGSGRDDKRP PPPYPGPGKP AAAAAWIQGP 910 920 930 940 950 960 PEGVETPTDQ GGQAAEREQQ VTQKKLSSAN SLPAGEQDSP RLGDAGWLDW QRERWQIWEL 970 LSTDNPDALP ETLV
	Isoform 1 [UniParc]. Checksum: 992B50A957E7019E		765	83,926
	10 20 30 40 50 60 MSAQSLLHSV FSCSSPASSS AASAKGFSKR KLRQTRSLDP ALIGGCGSDE AGAEGSARGA 70 80 90 100 110 120 TAGRLYSPSL PAESLGPRLA SSSRGPPPRA TRLPPPGPLC SSFSTPSTPQ EKSPSGSFHF 130 140 150 160 170 180 DYEVPLGRGG LKKSMAWDLP SVLAGPASSR SASSILCSSG GGPNGIFASP RRWLQQRKFQ 190 200 210 220 230 240 SPPDSRGHPY VVWKSEGDFT WNSMSGRSVR LRSVPIQSLS ELERARLQEV AFYQLQQDCD 250 260 270 280 290 300 LSCQITIPKD GQKRKKSLRK KLDSLGKEKN KDKEFIPQAF GMPLSQVIAN DRAYKLKQDL 310 320 330 340 350 360 QRDEQKDASD FVASLLPFGN KRQNKELSSS NSSLSSTSET PNESTSPNTP EPAPRARRRG 370 380 390 400 410 420 AMSVDSITDL DDNQSRLLEA LQLSLPAEAQ SKKEKARDKK LSLNPIYRQV PRLVDSCCQH 430 440 450 460 470 480 LEKHGLQTVG IFRVGSSKKR VRQLREEFDR GIDVSLEEEH SVHDVAALLK EFLRDMPDPL 490 500 510 520 530 540 LTRELYTAFI NTLLLEPEEQ LGTLQLLIYL LPPCNCDTLH RLLQFLSIVA RHADDNISKD 550 560 570 580 590 600 GQEVTGNKMT SLNLATIFGP NLLHKQKSSD KEFSVQSSAR AEESTAIIAV VQKMIENYEA 610 620 630 640 650 660 LFMVPPDLQN EVLISLLETD PDVVDYLLRR KASQSSSPDM LQSEVSFSVG GRHSSTDSNK 670 680 690 700 710 720 ASSGDISPYD NNSPVLSERS LLAMQEDAAP GGSEKLYRVP GQFMLVGHLS SSKSRESSPG 730 740 750 760 PRLGKGNWSL ASRRWPKQAT LLLLHVAWCG ALRTFSSSLP YLMFL
	Isoform 2 [UniParc]. Checksum: EE1801C208E48DB9		690	75,918
	10 20 30 40 50 60 MSAQSLLHSV FSCSSPASSS AASAKGFSKR KLRQTRSLDP ALIGGCGSDE AGAEGSARGA 70 80 90 100 110 120 TAGRLYSPSL PAESLGPRLA SSSRGPPPRA TRLPPPGPLC SSFSTPSTPQ EKSPSGSFHF 130 140 150 160 170 180 DYEVPLGRGG LKKSMAWDLP SVLAGPASSR SASSILCSSG GGPNGIFASP RRWLQQRKFQ 190 200 210 220 230 240 SPPDSRGHPY VVWKSELELY DLQILGTKPP MNSDTHRNFD PTATLRNQGD FTWNSMSGRS 250 260 270 280 290 300 VRLRSVPIQS LSELERARLQ EVAFYQLQQD CDLSCQITIP KDGQKRKKSL RKKLDSLGKE 310 320 330 340 350 360 KNKDKEFIPQ AFGMPLSQVI ANDRAYKLKQ DLQRDEQKDA SDFVASLLPF GNKRQNKELS 370 380 390 400 410 420 SSNSSLSSTS ETPNESTSPN TPEPAPRARR RGAMSVDSIT DLDDNQSRLL EALQLSLPAE 430 440 450 460 470 480 AQSKKEKARD KKLSLNPIYR QVPRLVDSCC QHLEKHGLQT VGIFRVGSSK KRVRQLREEF 490 500 510 520 530 540 DRGIDVSLEE EHSVHDVAAL LKEFLRDMPD PLLTRELYTA FINTLLLEPE EQLGTLQLLI 550 560 570 580 590 600 YLLPPCNCDT LHRLLQFLSI VARHADDNIS KDGQEVTGNK MTSLNLATIF GPNLLHKQKS 610 620 630 640 650 660 SDKEFSVQSS ARAEESTAII AVVQKMIENY EALFMVPPDL QNEVLISLLE TDPDVVDYLL 670 680 690 RRKASQSSTS SVLPAAVQAC PQYPASMFTP
	Isoform 4 [UniParc]. Checksum: A7CAEADB29590AAF		771	84,844
	10 20 30 40 50 60 MSGRSVRLRS VPIQSLSELE RARLQEVAFY QLQQDCDLSC QITIPKDGQK RKKSLRKKLD 70 80 90 100 110 120 SLGKEKNKDK EFIPQAFGMP LSQVIANDRA YKLKQDLQRD EQKDASDFVA SLLPFGNKRQ 130 140 150 160 170 180 NKELSSSNSS LSSTSETPNE STSPNTPEPA PRARRRGAMS VDSITDLDDN QSRLLEALQL 190 200 210 220 230 240 SLPAEAQSKK EKARDKKLSL NPIYRQVPRL VDSCCQHLEK HGLQTVGIFR VGSSKKRVRQ 250 260 270 280 290 300 LREEFDRGID VSLEEEHSVH DVAALLKEFL RDMPDPLLTR ELYTAFINTL LLEPEEQLGT 310 320 330 340 350 360 LQLLIYLLPP CNCDTLHRLL QFLSIVARHA DDNISKDGQE VTGNKMTSLN LATIFGPNLL 370 380 390 400 410 420 HKQKSSDKEF SVQSSARAEE STAIIAVVQK MIENYEALFM VPPDLQNEVL ISLLETDPDV 430 440 450 460 470 480 VDYLLRRKAS QSSSPDMLQS EVSFSVGGRH SSTDSNKASS GDISPYDNNS PVLSERSLLA 490 500 510 520 530 540 MQEDAAPGGS EKLYRVPGQF MLVGHLSSSK SRESSPGPRL GKDLSEEPFD IWGTWHSTLK 550 560 570 580 590 600 SGSKDPGMTG SSGDIFESSS LRAGPCSLSQ GNLSPNWPRW QGSPAELDSD TQGARRTQAA 610 620 630 640 650 660 APATEGRAHP AVSRACSTPH VQVAGKAERP TARSEQYLTL SGAHDLSESE LDVAGLQSRA 670 680 690 700 710 720 TPQCQRPHGS GRDDKRPPPP YPGPGKPAAA AAWIQGPPEG VETPTDQGGQ AAEREQQVTQ 730 740 750 760 770 KKLSSANSLP AGEQDSPRLG DAGWLDWQRE RWQIWELLST DNPDALPETL V
	Isoform 5 [UniParc]. Checksum: 96707FB1D7CBFC62		562	62,822
	10 20 30 40 50 60 MSGRSVRLRS VPIQSLSELE RARLQEVAFY QLQQDCDLSC QITIPKDGQK RKKSLRKKLD 70 80 90 100 110 120 SLGKEKNKDK EFIPQAFGMP LSQVIANDRA YKLKQDLQRD EQKDASDFVA SLLPFGNKRQ 130 140 150 160 170 180 NKELSSSNSS LSSTSETPNE STSPNTPEPA PRARRRGAMS VDSITDLDDN QSRLLEALQL 190 200 210 220 230 240 SLPAEAQSKK EKARDKKLSL NPIYRQVPRL VDSCCQHLEK HGLQTVGIFR VGSSKKRVRQ 250 260 270 280 290 300 LREEFDRGID VSLEEEHSVH DVAALLKEFL RDMPDPLLTR ELYTAFINTL LLEPEEQLGT 310 320 330 340 350 360 LQLLIYLLPP CNCDTLHRLL QFLSIVARHA DDNISKDGQE VTGNKMTSLN LATIFGPNLL 370 380 390 400 410 420 HKQKSSDKEF SVQSSARAEE STAIIAVVQK MIENYEALFM VPPDLQNEVL ISLLETDPDV 430 440 450 460 470 480 VDYLLRRKAS QSSSPDMLQS EVSFSVGGRH SSTDSNKASS GDISPYDNNS PVLSERSLLA 490 500 510 520 530 540 MQEDAAPGGS EKLYRVPGQF MLVGHLSSSK SRESSPGPRL GKGNWSLASR RWPKQATLLL 550 560 LHVAWCGALR TFSSSLPYLM FL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional analysis of ARHGAP6, a novel GTPase-activating protein for RhoA." Prakash S.K., Paylor R., Jenna S., Lamarche-Vane N., Armstrong D.L., Xu B., Mancini M.A., Zoghbi H.Y. Hum. Mol. Genet. 9:477-488(2000) [PubMed: 10699171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3; 4 AND 5), SEQUENCE REVISION, FUNCTION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING. Tissue: Fetal kidney.
[2]	"Cloning and characterization of a novel rho-type GTPase-activating protein gene (ARHGAP6) from the critical region for microphthalmia with linear skin defects." Schaefer L., Prakash S.K., Zoghbi H.Y. Genomics 46:268-277(1997) [PubMed: 9417914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[3]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264; SER-663; SER-673; SER-754; SER-777; SER-928 AND SER-939, MASS SPECTROMETRY. Tissue: Platelet.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF117067 mRNA. Translation: AAF43261.1. AF177663 mRNA. Translation: AAD53166.1. AF177665 mRNA. Translation: AAD55087.1. AF012272 mRNA. Translation: AAC98539.2. AF022212 mRNA. Translation: AAC98540.2.
PIR	E59434.
RefSeq	NP_006116.2. NP_038267.1. NP_038286.2.
UniGene	Hs.435291
3D structure databases
HSSP	HSSP built from PDB template 1F7C based on UniProtKB Q98935.
ModBase	Search...
PTM databases
PhosphoSite	O43182.
Genome annotation databases
Ensembl	ENSG00000047648. Homo sapiens. [Contig view]
GeneID	395.
KEGG	hsa:395.
Organism-specific databases
H-InvDB	HIX0056117.
HGNC	HGNC:676. ARHGAP6.
MIM	300118. gene.
PharmGKB	PA24960.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	O43182.
HOVERGEN	O43182.
Enzyme and pathway databases
Reactome	REACT_11044. Signaling by Rho GTPases.
Gene expression databases
ArrayExpress	O43182.
CleanEx	HS_ARHGAP6.
GermOnline	ENSG00000047648. Homo sapiens.
Family and domain databases
InterPro	IPR000198. RhoGAP. [Graphical view]
Gene3D	G3DSA:1.10.555.10. RhoGAP. 1 hit.
Pfam	PF00620. RhoGAP. 1 hit. [Graphical view]
SMART	SM00324. RhoGAP. 1 hit. [Graphical view]
PROSITE	PS50238. RHOGAP. 1 hit. [Graphical view]
ProDom	O43182. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

RHG06_HUMAN

Accession

Primary (citable) accession number: O43182
Secondary accession number(s): O43437 , Q9P1B3, Q9UK81, Q9UK82

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	October 3, 2006
Last modified:	July 22, 2008
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)