Reviewed, UniProtKB/Swiss-Prot O43572 (AKA10_HUMAN)

Names and origin

Protein names

Recommended name:
    A kinase anchor protein 10, mitochondrial
Alternative name(s):
    Protein kinase A-anchoring protein 10
      Short name=PRKA10
    Dual specificity A kinase-anchoring protein 2
      Short name=D-AKAP-2

Gene names

Name:

AKAP10

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	662 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase By similarity.
Subcellular location	Mitochondrion. Membrane. Cytoplasm. Note= Predominantly mitochondrial but also membrane associated and cytoplasmic. Ref.1
Domain	RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
Sequence similarities	Contains 2 RGS domains.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane Mitochondrion
Coding sequence diversity	Polymorphism
Domain	Repeat Transit peptide
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein localization Traceable author statement. Source: ProtInc signal transduction Traceable author statement. Source: ProtInc
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Traceable author statement. Source: ProtInc signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 28	28	Mitochondrion Potential
	Chain	29 – 662	634	A kinase anchor protein 10, mitochondrial		PRO_0000030404
Regions
	Domain	125 – 369	245	RGS 1
	Domain	379 – 505	127	RGS 2
	Region	634 – 647	14	PKA-RII subunit binding
Amino acid modifications
	Modified residue	52	1	Phosphoserine Ref.4
	Modified residue	187	1	Phosphoserine Ref.4
Natural variations
	Natural variant	249	1	R → H: dbSNP rs2108978.		VAR_024607
	Natural variant	646	1	I → V: dbSNP rs203462.		VAR_024608
Experimental info
	Sequence conflict	177	1	V → M in AAB92260. Ref.2
	Sequence conflict	524	1	L → P in AAB92260. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O43572-1 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: B7A6D92CA2D3D794	FASTA	662	73,818
	10 20 30 40 50 60 MRGAGPSPRQ SPRTLRPDPG PAMSFFRRKV KGKEQEKTSD VKSIKASISV HSPQKSTKNH 70 80 90 100 110 120 ALLEAAGPSH VAINAISANM DSFSSSRTAT LKKQPSHMEA AHFGDLGRSC LDYQTQETKS 130 140 150 160 170 180 SLSKTLEQVL HDTIVLPYFI QFMELRRMEH LVKFWLEAES FHSTTWSRIR AHSLNTVKQS 190 200 210 220 230 240 SLAEPVSPSK KHETTASFLT DSLDKRLEDS GSAQLFMTHS EGIDLNNRTN STQNHLLLSQ 250 260 270 280 290 300 ECDSAHSLRL EMARAGTHQV SMETQESSST LTVASRNSPA SPLKELSGKL MKSIEQDAVN 310 320 330 340 350 360 TFTKYISPDA AKPIPITEAM RNDIIARICG EDGQVDPNCF VLAQSIVFSA MEQEHFSEFL 370 380 390 400 410 420 RSHHFCKYQI EVLTSGTVYL ADILFCESAL FYFSEYMEKE DAVNILQFWL AADNFQSQLA 430 440 450 460 470 480 AKKGQYDGQE AQNDAMILYD KYFSLQATHP LGFDDVVRLE IESNICREGG PLPNCFTTPL 490 500 510 520 530 540 RQAWTTMEKV FLPGFLSSNL YYKYLNDLIH SVRGDEFLGG NVSLTAPGSV GPPDESHPGS 550 560 570 580 590 600 SDSSASQSSV KKASIKILKN FDEAIIVDAA SLDPESLYQR TYAGKMTFGR VSDLGQFIRE 610 620 630 640 650 660 SEPEPDVRKS KGSMFSQAMK KWVQGNTDEA QEELAWKIAK MIVSDIMQQA QYDQPLEKST KL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and mitochondrial localization of full-length D-AKAP2, a protein kinase A anchoring protein." Wang L., Sunahara R.K., Krumins A., Perkins G., Crochiere M.L., Mackey M., Bell S., Ellisman M.H., Taylor S.S. Proc. Natl. Acad. Sci. U.S.A. 98:3220-3225(2001) [PubMed: 11248059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION. Tissue: Brain.
[2]	"Homo sapiens protein kinase A anchoring protein with an RGS domain." Chatterjee T.K., Fisher R.A. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[4]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52 AND SER-187, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF037439 mRNA. Translation: AAB92260.1. BC017055 mRNA. Translation: AAH17055.1.
RefSeq	NP_009133.2.
UniGene	Hs.694769
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O43572. 1 interaction.
PTM databases
PhosphoSite	O43572.
Proteomic databases
PRIDE	O43572.
Genome annotation databases
Ensembl	ENSG00000108599. Homo sapiens. [Contig view]
GeneID	11216.
KEGG	hsa:11216.
Organism-specific databases
GeneCards	GC17M019749.
H-InvDB	HIX0017647.
HGNC	HGNC:368. AKAP10.
MIM	604694. gene.
PharmGKB	PA24662.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O43572.
HOVERGEN	O43572.
Gene expression databases
ArrayExpress	O43572.
CleanEx	HS_AKAP10.
GermOnline	ENSG00000108599. Homo sapiens.
Family and domain databases
InterPro	IPR000342. Regulat_G_prot_signal. [Graphical view]
SMART	SM00315. RGS. 2 hits. [Graphical view]
PROSITE	PS50132. RGS. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	42691.
SOURCE	Search...

Entry information

Entry name

AKA10_HUMAN

Accession

Primary (citable) accession number: O43572
Secondary accession number(s): Q96AJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	July 19, 2005
Last modified:	December 16, 2008
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)