Mitochondrial import inner membrane translocase subunit TIM44 precursor

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Reviewed, UniProtKB/Swiss-Prot O43615 (TIM44_HUMAN)

Last modified December 16, 2008. Version 76. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit TIM44

Gene names

Name:	TIMM44
Synonyms:	MIMT44, TIM44

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.
Subunit structure	Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/MAGMAS and TIMM14/DNAJC19 By similarity. The complex interacts with the TIMM23 component of the TIM23 complex.
Subcellular location	Mitochondrion inner membrane.
Sequence similarities	Belongs to the Tim44 family.

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Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Technical term	3D-structure
Gene Ontology (GO)
Biological process	intracellular protein transmembrane transport Inferred from electronic annotation. Source: UniProtKB-KW protein targeting to mitochondrion Ref.1 Traceable author statement. Source: ProtInc
Cellular component	mitochondrial inner membrane presequence translocase complex Inferred from electronic annotation. Source: InterPro mitochondrial matrix Ref.1 Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW P-P-bond-hydrolysis-driven protein transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARAF	P10398	3	EBI-861737,EBI-365961

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion Potential

Chain

? – 452

Mitochondrial import inner membrane translocase subunit TIM44

PRO_0000034314

Regions

Nucleotide binding

166 – 173

ATP Potential

Experimental info

Sequence conflict

189

E → A in AAB97740. Ref.1

Sequence conflict

226

P → A in AAB97740. Ref.1

Secondary structure

452

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

O43615-1 [UniParc].

Last modified November 8, 2002. Version 2.
Checksum: 15BECF875611BE96
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FASTA

452

51,356

        10         20         30         40         50         60 
MAAAALRSGW CRCPRRCLGS GIQFLSSHNL PHGSTYQMRR PGGELPLSKS YSSGNRKGFL 

        70         80         90        100        110        120 
SGLLDNVKQE LAKNKEMKES IKKFRDEARR LEESDVLQEA RRKYKTIESE TVRTSEVLRK 

       130        140        150        160        170        180 
KLGELTGTVK ESLHEVSKSD LGRKIKEGVE EAAKTAKQSA ESVSKGGEKL GRTAAFRALS 

       190        200        210        220        230        240 
QGVESVKKEI DDSVLGQTGP YRRPQRLRKR TEFAGDKFKE EKVFEPNEEA LGVVLHKDSK 

       250        260        270        280        290        300 
WYQQWKDFKE NNVVFNRFFE MKMKYDESDN AFIRASRALT DKVTDLLGGL FSKTEMSEVL 

       310        320        330        340        350        360 
TEILRVDPAF DKDRFLKQCE NDIIPNVLEA MISGELDILK DWCYEATYSQ LAHPIQQAKA 

       370        380        390        400        410        420 
LGLQFHSRIL DIDNVDLAMG KMMEQGPVLI ITFQAQLVMV VRNPKGEVVE GDPDKVLRML 

       430        440        450 
YVWALCRDQD ELNPYAAWRL LDISASSTEQ IL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic and structural characterization of the human mitochondrial inner membrane translocase." Bauer M.F., Gempel K., Reichert A.S., Rappold G.A., Lichtner P., Gerbitz K.-D., Neupert W., Brunner M., Hofmann S. J. Mol. Biol. 289:69-82(1999) [PubMed: 10339406] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.

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Cross-references

Sequence databases

AF041254 mRNA. Translation: AAB97740.1.
BC033628 mRNA. Translation: AAH33628.1.

RefSeq

NP_006342.2.

UniGene

Hs.465784

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CW9	X-ray	1.90	A	266-452	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

O43615. 1 interaction.

PTM databases

PhosphoSite

O43615.

Proteomic databases

PeptideAtlas

O43615.

PRIDE

O43615.

Genome annotation databases

Ensembl

ENSG00000104980. Homo sapiens. [Contig view]

GeneID

10469.

KEGG

hsa:10469.

Organism-specific databases

GeneCards

GC19M007897.

H-InvDB

HIX0022207.

HGNC

HGNC:17316. TIMM44.

MIM

605058. gene.

PharmGKB

PA38229.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

O43615.

HOVERGEN

O43615.

Gene expression databases

ArrayExpress

O43615.

CleanEx

HS_TIMM44.

GermOnline

ENSG00000104980. Homo sapiens.

Family and domain databases

InterPro

IPR017303. Import_IM_translocase_TIM44_mt.
IPR007379. Tim44-like.
IPR005682. Tim44_sub.
[Graphical view]

Pfam

PF04280. Tim44. 1 hit.
[Graphical view]

PIRSF

PIRSF037871. TIM44. 1 hit.

TIGRFAMs

TIGR00984. 3a0801s03tim44. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

39705.

SOURCE

Search...

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Entry information

Entry name

TIM44_HUMAN

Accession

Primary (citable) accession number: O43615
Secondary accession number(s): Q8N193

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 8, 2002
Last modified:	December 16, 2008
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents