Reviewed, UniProtKB/Swiss-Prot O43791 (SPOP_HUMAN)

Names and origin

Protein names

Recommended name:
    Speckle-type POZ protein
Alternative name(s):
    HIB homolog 1
    Roadkill homolog 1

Gene names

Name:

SPOP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex By similarity. In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Homodimer. Part of a complex consisting of BMI1, CUL3 and SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Part of a complex consisting of DAXX, CUL3 and SPOP. Interacts with H2AFY, IPF1/PDX1, BMI1 and DAXX. Interacts with CUL3. Ref.4 Ref.5 Ref.6
Subcellular location	Nucleus. Ref.6
Tissue specificity	Widely expressed.
Domain	The MATH domain interacts with H2AFY and BMI1.
Miscellaneous	Antigen recognized by serum from scleroderma patient.
Sequence similarities	Belongs to the Tdpoz family. Contains 1 BTB (POZ) domain. Contains 1 MATH domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Technical term	3D-structure
Gene Ontology (GO)
Biological process	mRNA processing Ref.1 Traceable author statement. Source: ProtInc ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DOK2	O60496	1	EBI-743549,EBI-1046024
GDI1	P31150	1	EBI-743549,EBI-946999
KPNA6	O60684	1	EBI-743549,EBI-359923
PIP5K1B	O14986	1	EBI-743549,EBI-1053734
SIRT2	Q8IXJ6	1	EBI-743549,EBI-477232

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 374

374

Speckle-type POZ protein

PRO_0000191621

Regions

Domain

31 – 161

131

MATH

Domain

173 – 297

125

BTB

Region

71 – 191

121

Required for nuclear localization

Region

297 – 355

59

Homodimerization

Experimental info

Sequence conflict

239

1

N → S in BAD96309. Ref.2

Secondary structure

1

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374

Helix Strand Turn

Details...

Beta strand

29 – 39

11

Helix

41 – 43

3

Beta strand

59 – 61

3

Beta strand

67 – 71

5

Beta strand

76 – 83

8

Beta strand

86 – 89

4

Beta strand

94 – 101

8

Beta strand

103 – 107

5

Beta strand

113 – 118

6

Beta strand

123 – 126

4

Beta strand

130 – 134

5

Helix

139 – 142

4

Turn

145 – 148

4

Beta strand

153 – 164

12

Sequences

	Sequence		Length	Mass (Da)
	O43791-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: EE5F4C5CF6FD09DC	FASTA	374	42,132
	10 20 30 40 50 60 MSRVPSPPPP AEMSSGPVAE SWCYTQIKVV KFSYMWTINN FSFCREEMGE VIKSSTFSSG 70 80 90 100 110 120 ANDKLKWCLR VNPKGLDEES KDYLSLYLLL VSCPKSEVRA KFKFSILNAK GEETKAMESQ 130 140 150 160 170 180 RAYRFVQGKD WGFKKFIRRD FLLDEANGLL PDDKLTLFCE VSVVQDSVNI SGQNTMNMVK 190 200 210 220 230 240 VPECRLADEL GGLWENSRFT DCCLCVAGQE FQAHKAILAA RSPVFSAMFE HEMEESKKNR 250 260 270 280 290 300 VEINDVEPEV FKEMMCFIYT GKAPNLDKMA DDLLAAADKY ALERLKVMCE DALCSNLSVE 310 320 330 340 350 360 NAAEILILAD LHSADQLKTQ AVDFINYHAS DVLETSGWKS MVVSHPHLVA EAYRSLASAQ 370 CPFLGPPRKR LKQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel nuclear speckle-type protein, SPOP." Nagai Y., Kojima T., Muro Y., Hachiya T., Nishizawa Y., Wakabayashi T., Hagiwara M. FEBS Lett. 418:23-26(1997) [PubMed: 9414087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Coronary artery.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix and Placenta.
[4]	"Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases." Furukawa M., He Y.J., Borchers C., Xiong Y. Nat. Cell Biol. 5:1001-1007(2003) [PubMed: 14528312] [Abstract] Cited for: FUNCTION AS A E3 UBIQUITIN-PROTEIN LIGASE, INTERACTION WITH CUL3.
[5]	"Daxx-mediated transcriptional repression of MMP1 gene is reversed by SPOP." La M., Kim K., Park J., Won J., Lee J.-H., Fu Y.M., Meadows G.G., Joe C.O. Biochem. Biophys. Res. Commun. 320:760-765(2004) [PubMed: 15240113] [Abstract] Cited for: INTERACTION WITH DAXX, HOMODIMERIZATION.
[6]	"Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase." Hernandez-Munoz I., Lund A.H., van der Stoop P., Boutsma E., Muijrers I., Verhoeven E., Nusinow D.A., Panning B., Marahrens Y., van Lohuizen M. Proc. Natl. Acad. Sci. U.S.A. 102:7635-7640(2005) [PubMed: 15897469] [Abstract] Cited for: INTERACTION WITH BMI1, IDENTIFICATION IN A COMPLEX WITH CUL3 AND BMI1, IDENTIFICATION IN A COMPLEX WITH CUL3 AND H2AFY, SUBCELLULAR LOCATION, FUNCTION.
[7]	"Roadkill attenuates Hedgehog responses through degradation of Cubitus interruptus." Kent D., Bush E.W., Hooper J.E. Development 133:2001-2010(2006) [PubMed: 16651542] [Abstract] Cited for: IDENTIFICATION.
[8]	"A hedgehog-induced BTB protein modulates hedgehog signaling by degrading Ci/Gli transcription factor." Zhang Q., Zhang L., Wang B., Ou C.-Y., Chien C.-T., Jiang J. Dev. Cell 10:719-729(2006) [PubMed: 16740475] [Abstract] Cited for: IDENTIFICATION.
[9]	"BTB domain-containing speckle-type POZ protein (SPOP) serves as an adaptor of Daxx for ubiquitination by Cul3-based ubiquitin ligase." Kwon J.E., La M., Oh K.H., Oh Y.M., Kim G.R., Seol J.H., Baek S.H., Chiba T., Tanaka K., Bang O.S., Joe C.O., Chung C.H. J. Biol. Chem. 281:12664-12672(2006) [PubMed: 16524876] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH CUL3 AND DAXX, FUNCTION.
[10]	"Solution structure of N-terminal domain of speckle-type POZ protein." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 28-173.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AJ000644 mRNA. Translation: CAA04199.1.
AK222589 mRNA. Translation: BAD96309.1.
BC001269 mRNA. Translation: AAH01269.1.
BC003385 mRNA. Translation: AAH03385.1.

RefSeq

NP_001007227.1.
NP_001007228.1.
NP_001007229.1.
NP_001007230.1.
NP_001007231.1.
NP_003554.1.

UniGene

Hs.463382

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CR2	NMR	-	A	28-173	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

O43791. 11 interactions.

PTM databases

PhosphoSite

O43791.

Proteomic databases

PRIDE

O43791.

Genome annotation databases

Ensembl

ENSG00000121067. Homo sapiens. [Contig view]

GeneID

8405.

KEGG

hsa:8405.

Organism-specific databases

GeneCards

GC17M045032.

H-InvDB

HIX0002485.
HIX0013962.

HGNC

HGNC:11254. SPOP.

MIM

602650. gene.

PharmGKB

PA36084.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

O43791.

HOVERGEN

O43791.

Gene expression databases

ArrayExpress

O43791.

CleanEx

HS_SPOP.

GermOnline

ENSG00000121067. Homo sapiens.

Family and domain databases

InterPro

IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR013089. Kelch_related.
IPR002083. MATH.
[Graphical view]

Gene3D

G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit.

PANTHER

PTHR23230. Kelch_related. 1 hit.

Pfam

PF00651. BTB. 1 hit.
PF00917. MATH. 1 hit.
[Graphical view]

SMART

SM00225. BTB. 1 hit.
SM00061. MATH. 1 hit.
[Graphical view]

PROSITE

PS50097. BTB. 1 hit.
PS50144. MATH. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

31454.

SOURCE

Search...

Entry information

Entry name

SPOP_HUMAN

Accession

Primary (citable) accession number: O43791
Secondary accession number(s): Q53HJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	December 16, 2008
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)