Reviewed,
UniProtKB/Swiss-Prot O45947 (GLT10_CAEEL)
Last modified
November 4, 2008.
Version 59.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (3) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Putative polypeptide N-acetylgalactosaminyltransferase 10 Short name=pp-GaNTase 10 EC=2.4.1.41 Alternative name(s): Protein-UDP acetylgalactosaminyltransferase 10 UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10 | ||||
| Gene names |
| ||||
| Organism | Caenorhabditis elegans [Complete proteome] | ||||
| Taxonomic identifier | 6239 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Protein attributes
| Sequence length | 684 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | May catalyze the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor By similarity. |
| Catalytic activity | UDP-N-acetyl-D-galactosamine + polypeptide = UDP + N-acetyl-D-galactosaminyl-polypeptide. |
| Cofactor | Manganese By similarity. Calcium By similarity. |
| Pathway | |
| Subcellular location | Golgi apparatus membrane; Single-pass type II membrane proteinBy similarity. |
| Domain | There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity. The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity. |
| Sequence similarities | Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Golgi apparatus Membrane |
| Coding sequence diversity | Alternative splicing |
| Domain | Signal-anchor Transmembrane |
| Ligand | Calcium Lectin Manganese Metal-binding |
| Molecular function | Glycosyltransferase Transferase |
| PTM | Glycoprotein |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Cellular component | Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW polypeptide N-acetylgalactosaminyltransferase activityInferred from electronic annotation. Source: EC sugar bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform a (identifier: O45947-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Notes: No experimental confirmation available. | ||||||
| Isoform b (identifier: O45947-2) The sequence of this isoform differs from the canonical sequence as follows: 1-62: Missing. | ||||||
| Notes: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 684 | 684 | Putative polypeptide N-acetylgalactosaminyltransferase 10 | PRO_0000059153 | |||||||
Regions | |||||||||||
| Topological domain | 1 – 74 | 74 | Cytoplasmic Potential | ||||||||
| Transmembrane | 75 – 95 | 21 | Signal-anchor for type II membrane protein Potential | ||||||||
| Topological domain | 96 – 684 | 589 | Lumenal Potential | ||||||||
| Domain | 581 – 684 | 104 | Ricin B-type lectin | ||||||||
| Region | 218 – 330 | 113 | Catalytic subdomain A | ||||||||
| Region | 386 – 448 | 63 | Catalytic subdomain B | ||||||||
| Region | 448 – 461 | 14 | Flexible loop By similarity | ||||||||
Sites | |||||||||||
| Metal binding | 314 | 1 | Manganese By similarity | ||||||||
| Metal binding | 316 | 1 | Manganese By similarity | ||||||||
| Metal binding | 445 | 1 | Manganese By similarity | ||||||||
| Binding site | 259 | 1 | Substrate By similarity | ||||||||
| Binding site | 314 | 1 | Substrate By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 198 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 232 | 1 | N-linked (GlcNAc...) Ref.3 | ||||||||
| Disulfide bond | 209 ↔ 440 | By similarity | |||||||||
| Disulfide bond | 431 ↔ 509 | By similarity | |||||||||
| Disulfide bond | 594 ↔ 611 | By similarity | |||||||||
| Disulfide bond | 637 ↔ 653 | By similarity | |||||||||
Natural variations | |||||||||||
| Alternative sequence | 1 – 62 | 62 | Missing in isoform b. | VSP_032724 | |||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2. |
| [2] | "The terminal phase of cytokinesis in the Caenorhabditis elegans early embryo requires protein glycosylation." Wang H., Spang A., Sullivan M.A., Hryhorenko J., Hagen F.K. Mol. Biol. Cell 16:4202-4213(2005) [PubMed: 15987734] [Abstract] Cited for: IDENTIFICATION. |
| [3] | "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-232, MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| AL021492 Genomic DNA. Translation: CAA16378.1. AL021492 Genomic DNA. Translation: CAJ76949.1. | |
| PIR | T26930. |
| RefSeq | NP_001041036.1. |
| UniGene | Cel.6680 |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| Ensembl | Y45F10D.3. Caenorhabditis elegans. [Contig view] |
| GeneID | 178343. |
| KEGG | cel:Y45F10D.3. |
Organism-specific databases | |
| WormBase | WBGene00012884. Y45F10D.3. |
| WormPep | Y45F10D.3a. CE16642. [WorfDB] Y45F10D.3b. CE39841. [WorfDB] |
Gene expression databases | |
| ArrayExpress | O45947. |
Family and domain databases | |
| InterPro | IPR001173. Glyco_trans_2. IPR000772. Ricin_B_lectin. [Graphical view] |
| Pfam | PF00535. Glycos_transf_2. 1 hit. PF00652. Ricin_B_lectin. 1 hit. [Graphical view] |
| SMART | SM00458. RICIN. 1 hit. [Graphical view] |
| PROSITE | PS50231. RICIN_B_LECTIN. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 900740. |
Entry information
| Entry name | GLT10_CAEEL | ||||||||
| Accession | Primary (citable) accession number: O45947 Secondary accession number(s): Q2HQM0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Caenorhabditis annotation project | ||||||||
Relevant documents
| Caenorhabditis elegans Caenorhabditis elegans: entries, gene names and cross-references to WormPep |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
