Reviewed, UniProtKB/Swiss-Prot O50089 (IF5A_PYRHO)

Names and origin

Protein names

Recommended name:
    Translation initiation factor 5A
Alternative name(s):
    eIF-5A
    Hypusine-containing protein
    PhoIF-5A

Gene names

Name:	eif5a
Ordered Locus Names:	PH1381

Organism

Pyrococcus horikoshii [Complete proteome] [HAMAP]

Taxonomic identifier

53953 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions by promoting the formation of the first peptide bond By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00085
Sequence similarities	Belongs to the eIF-5A family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Initiation factor
PTM	Hypusine
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	translational initiation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 138

138

Translation initiation factor 5A HAMAP MF_00085

PRO_0000142501

Amino acid modifications

Modified residue

37

1

Hypusine HAMAP MF_00085

Secondary structure

1

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138

Helix Strand Turn

Details...

Beta strand

4 – 8

5

Helix

9 – 11

3

Beta strand

16 – 20

5

Beta strand

23 – 32

10

Beta strand

42 – 49

8

Turn

50 – 52

3

Beta strand

55 – 61

7

Beta strand

64 – 69

6

Beta strand

72 – 81

10

Beta strand

83 – 89

7

Turn

91 – 93

3

Beta strand

96 – 100

5

Helix

101 – 104

4

Helix

107 – 109

3

Turn

110 – 112

3

Beta strand

118 – 124

7

Beta strand

127 – 133

7

Sequences

	Sequence		Length	Mass (Da)
	O50089-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: B736249262E129B0	FASTA	138	15,285
	10 20 30 40 50 60 MGDKTKVQVS KLKPGRYIII DDEPCRIVNI TVSSPGKHGS AKARIEAVGI FDGKVRSIVK 70 80 90 100 110 120 PTSAEVDVPI IDKKTAQVIA ITPDTVQIMD METYETFEVP IDTGVADEIR DQLKEGINVE 130 YWETLGRIKI MRIKGEGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3." Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S., Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K., Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T., Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H. DNA Res. 5:55-76(1998) [PubMed: 9679194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OT3.
[2]	"Crystal structure of hyperthermophilic archaeal initiation factor 5A: a homologue of eukaryotic initiation factor 5A (eIF-5A)." Yao M., Ohsawa A., Kikukawa S., Tanaka I., Kimura M. J. Biochem. 133:75-81(2003) [PubMed: 12761201] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), HYPUSINE AT LYS-37. Strain: OT3.

Cross-references

Sequence databases

EMBLGenBankDDBJ

BA000001 Genomic DNA. Translation: BAA30487.1.

PIR

G71010.

RefSeq

NP_143260.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IZ6	X-ray	2.00	A/B/C	1-138	[»]

ModBase

Search...

Genome annotation databases

GeneID

1443708.

GenomeReviews

Gene locus PH1381 in contig BA000001_GR.

KEGG

pho:PH1381.

NMPDR

fig|70601.1.peg.1361.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

O50089.

Family and domain databases

HAMAP

MF_00085.
[Tree]

InterPro

IPR001884. EIF5A_hypusine.
IPR012340. NA-bd_OB-fold.
IPR014722. Transl_SH3_like_sub.
[Graphical view]

Gene3D

G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:2.30.30.30. Ribosomal_L2. 1 hit.

PANTHER

PTHR11673. EIF5A_hypusine. 1 hit.

Pfam

PF01287. eIF-5a. 1 hit.
[Graphical view]

PIRSF

PIRSF003025. eIF5A. 1 hit.

TIGRFAMs

TIGR00037. eIF_5A. 1 hit.

PROSITE

PS00302. IF5A_HYPUSINE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

IF5A_PYRHO

Accession

Primary (citable) accession number: O50089

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	July 22, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)