Reviewed, UniProtKB/Swiss-Prot O51879 (MNMG_BUCAP)

Names and origin

Protein names

Recommended name:
    tRNA uridine 5-carboxymethylaminomethyl modification enzyme mnmG
Alternative name(s):
    Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	BUsg_001

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	631 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 By similarity.
Cofactor	FAD By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two mnmE and two mnmG subunits By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the mnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 631	631	tRNA uridine 5-carboxymethylaminomethyl modification enzyme mnmG
Regions
	Nucleotide binding	15 – 20	6	FAD By similarity
	Nucleotide binding	275 – 289	15	NAD Potential
Sites
	Binding site	127	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	182	1	FAD By similarity
	Binding site	372	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O51879-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: DBBD71972F3370A3	FASTA	631	70,796
	10 20 30 40 50 60 MSKSYLKNFD VIVIGGGHAG TEAAAASARV GCKTLLLTQK ITDIGVLSCN PAIGGIGKSH 70 80 90 100 110 120 LVKEIDALGG LMARAIDYSG IQFRVLNSKK GPAVRSTRAQ ADRILYCQNI KKLLKKELNL 130 140 150 160 170 180 LILETEVKDL IVKNYQVIGV LTQSNMSFYS RSVVLSTGTF LGGKIHIGLE SYSAGRKGEK 190 200 210 220 230 240 ASIDLALRLR DLPFRVDRLK TGTPPRIDIN TINFENLFVQ HGDVPTPVFS FMGDISNHPL 250 260 270 280 290 300 QIPCFLTHTN EKTHEIIRKN LHKSPLYTGI IKGVGPRYCP SIEDKIVRFP DRKSHQIFLE 310 320 330 340 350 360 PEGLTSIEIY PNGISTSLPL DVQKEIVSSV KGLEKSKIIT PGYAVEYDFF DPKDLNLTLE 370 380 390 400 410 420 SKWIKGLFLA GQINGTTGYE EAASQGLLAG LNAALSAKDC KQWFPRRDQA YLGVLIDDLT 430 440 450 460 470 480 TQGANEPYRM FTSRSEYRLT LREDNADLRL TEIAYKLGLV DNPRWIRYNE KVLNISNEKN 490 500 510 520 530 540 RLKKIKIYPK SSDSTILNQL FNIVLTKEIN ILNLLKRPEI TYENLKYLKN FKVGISDLEA 550 560 570 580 590 600 AGQIENEIKY EGYIKRQLEE INRHLKNENT PLLPTYDYNK IKGLSHEAVL KLNDYKPVSV 610 620 630 GQASRISGIT PATISILLIH LKKEYYKNHL S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 34.7-kb DNA segment from the genome of Buchnera aphidicola (endosymbiont of aphids) containing groEL, dnaA, the atp operon, gidA, and rho." Clark M.A., Baumann L., Baumann P. Curr. Microbiol. 36:158-163(1998) [PubMed: 9516544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF008210 Genomic DNA. Translation: AAC38117.1. AE013218 Genomic DNA. Translation: AAM67573.1.
RefSeq	NP_660362.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1005814.
GenomeReviews	Gene locus BUsg_001 in contig AE013218_GR.
KEGG	bas:BUsg001.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O51879.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG001-MON.
Family and domain databases
HAMAP	MF_00129. [Tree]
InterPro	IPR002218. GIDA. IPR004416. GidA_sub. [Graphical view]
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00136. gidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MNMG_BUCAP

Accession

Primary (citable) accession number: O51879

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	July 22, 2008
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)