Reviewed, UniProtKB/Swiss-Prot O52702 (MTA1_ACEPA)

Names and origin

Protein names

Recommended name:
    Modification methylase ApaLI
      Short name=M.ApaLI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase ApaLI

Gene names

Name:

apaLIM

Organism

Acetobacter pasteurianus (Acetobacter turbidans)

Taxonomic identifier

438 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acetobacter

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GTGCAC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the ApaLI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 429	429	Modification methylase ApaLI
Sites
	Active site	81	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O52702-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: E011C7D15B33F5F3	FASTA	429	46,547
	10 20 30 40 50 60 MNKDEVVVSL FAGAGGFSSG FSQAGLKPLF GAEINADACQ TYQENVGSPC HQLDLSTVDP 70 80 90 100 110 120 SHIEMLTGGK RPFVVIGGPP CQGFSTAGPR NFADPRNLLI FNYLNIVERL SPRWLIFENV 130 140 150 160 170 180 EGLLTSGGGR DLARLVREFV DMGYSVRLQK VNLAAYGVPQ TRKRVLIIGN RLGIDFQFPE 190 200 210 220 230 240 ELYSFDSGKA KKASGKPLAP SLAEAVAGLG PAASDKDALV PYASSEPVNA FDARMRAGNR 250 260 270 280 290 300 VEVVTHHVRV EAAERMQVEL LKPGQTMKDL PPELWHESYR RRANRRVSDG TPTEKRGGAP 310 320 330 340 350 360 SGIKRLHGNL QSLTITGPAA REFIHPTEHR PLTIRECARI QTFPDKYRWV GNNASVIQQI 370 380 390 400 410 420 GNAVPPLAAE RLAKHLRDID GSFGADTRPA GAMSAKLLGF VLTEALGMSP ALKSTEALLA EMHQGGFVF

References

[1]

"Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli." Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II Mol. Gen. Genet. 260:226-231(1998) [PubMed: 9862476] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 12875.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF044847 Genomic DNA. Translation: AAC97180.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3281. M.ApaLI.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 2 hits. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. False negative. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MTA1_ACEPA

Accession

Primary (citable) accession number: O52702

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	June 1, 1998
Last modified:	September 2, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)